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The map label for this gene is bmr3 [H]

Identifier: 226223599

GI number: 226223599

Start: 1012920

End: 1014395

Strand: Reverse

Name: bmr3 [H]

Synonym: Lm4b_01001

Alternate gene names: 226223599

Gene position: 1014395-1012920 (Counterclockwise)

Preceding gene: 226223612

Following gene: 226223595

Centisome position: 34.83

GC content: 39.23

Gene sequence:

>1476_bases
TTGGAACAAACAAAAGTAAAAGCCGTTACCATCGCCGTATTTGTCGCAACATTTATGGCAGCTATTGAAGGTACCATCGT
GAGTACTGCGATGCCAACGATTGTAAGTCAGTTGGACGGCATAGAGTTAATGAACTGGATTTTTTCTGTTTATTTGCTAA
CATCAGCAGTGACGGTTCCGATTTACGGAAAACTGTCTGATTTGTATGGACGAAAAAATATTTTTGTGATTGCGACAATT
ATTTTTATTATTGGTTCATCACTTTGTGGATTTGCACAAAATATGGAACAATTAATTATTTTTCGCGCGATCCAAGGTAT
TGGAGCAGGTGGGATTTTACCATCTACTATGACGATTATCGCAGACGTGTATCCATTTGAAAAACGAGCAAAAGTACTTG
GCTTCATGGGTTCCGCTTGGGGTATCGCTGGCGTATTTGGACCACTTGTTGGAGGATTTTTAGTAGACCAACTTTCATGG
CATTGGATTTTCTTCATCAATGTCCCAATCGGTATTTTAACAATCCTACTTATTTTGCTTTATTTACGCGAAAAAGTGGA
ACATGTCAAACTACCAATTGACTATTTAGGTGCCTCGTTCTTTACGGTGGCTCTGCTTGGATTATTATTTGCGTTACAGC
GAGCCGGGGAATCACTCAACTGGACAGAACCGCTCGTCGTTATTCTTTTTGCCGTTTCGATTGTGTTATTTATTGCTTTT
TATTTTGTCGAAAAACGTGCAAAAGATCCGATTATGCCATTTGTATTATTTAAAAACCCAGTGGTGTTAATTGGGAACTT
AATTGGTTTCTTGATTAGTGCCTTCCTAATTGGAATTAACGTGTATATTCCAATGTGGGCACAAGGAATGCTTGGGCACG
GGGCTACGATTGCTGGATTTATGCTTGCACCGCTTTCAGTTACATGGATAATTGGTTCATTTATCGGCGGCAAACTTTTA
ATGACGCTTGGAAATCGGCATACGGTTGGCATTGGCGTTTTAATTACTGCTGCAAGTGGATTAATTTTAGCCTTATTCCC
CGCTTCCACAAATGATATCTTCTTCTATTTAAATAGTGCATTTATGGGATTTGGTTTTGGGATAATCTTTACAACAACAA
CTGTTACCGTTCAAGATGCAGTACCGCGATTCCAAACTGGTATTGCTACAGCTTCTAACACACTGTTTAGAACAGTTGGT
CAAACAATCGGGGTCGCTGTCTTTGGAACTATCTTCAACTCAGTTCTTACAAGCGAATTCCGCGCTGCAGCTGGCAGTGA
AGTAAATCGTAGTAATCTAAACCAACTAATTAGCCCACAAACTTCTGGCAACGTTAGTGCAGACCTTGTTGATCCGCTTC
GAGATATTTTATATAGCGGGCTTCATATGGTTTTCTGGGTTATGTTTGCTTGCTGTATCGTTAGTTATTTTATTCATTTT
ATCTTACCGAAAAAACATATTTCCGGTGAAAAATAA

Upstream 100 bases:

>100_bases
AGCGCTTATCTTGAATATGCGCATTTCTTAGGATTAACTTTTTTCCCAATTTAGTCCTTTTATATGCTATAATTCGGTAG
ACGAAAAGGGAGGGAATTTT

Downstream 100 bases:

>100_bases
AAAGTTTGGCTCCGCGGAGCCAAACTTTTTATTTTATACCATTTTTTTATAAATAGTTATCGCAAAAACATACGCAACAA
CGGTAATTCCGACACACCAA

Product: efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MEQTKVKAVTIAVFVATFMAAIEGTIVSTAMPTIVSQLDGIELMNWIFSVYLLTSAVTVPIYGKLSDLYGRKNIFVIATI
IFIIGSSLCGFAQNMEQLIIFRAIQGIGAGGILPSTMTIIADVYPFEKRAKVLGFMGSAWGIAGVFGPLVGGFLVDQLSW
HWIFFINVPIGILTILLILLYLREKVEHVKLPIDYLGASFFTVALLGLLFALQRAGESLNWTEPLVVILFAVSIVLFIAF
YFVEKRAKDPIMPFVLFKNPVVLIGNLIGFLISAFLIGINVYIPMWAQGMLGHGATIAGFMLAPLSVTWIIGSFIGGKLL
MTLGNRHTVGIGVLITAASGLILALFPASTNDIFFYLNSAFMGFGFGIIFTTTTVTVQDAVPRFQTGIATASNTLFRTVG
QTIGVAVFGTIFNSVLTSEFRAAAGSEVNRSNLNQLISPQTSGNVSADLVDPLRDILYSGLHMVFWVMFACCIVSYFIHF
ILPKKHISGEK

Sequences:

>Translated_491_residues
MEQTKVKAVTIAVFVATFMAAIEGTIVSTAMPTIVSQLDGIELMNWIFSVYLLTSAVTVPIYGKLSDLYGRKNIFVIATI
IFIIGSSLCGFAQNMEQLIIFRAIQGIGAGGILPSTMTIIADVYPFEKRAKVLGFMGSAWGIAGVFGPLVGGFLVDQLSW
HWIFFINVPIGILTILLILLYLREKVEHVKLPIDYLGASFFTVALLGLLFALQRAGESLNWTEPLVVILFAVSIVLFIAF
YFVEKRAKDPIMPFVLFKNPVVLIGNLIGFLISAFLIGINVYIPMWAQGMLGHGATIAGFMLAPLSVTWIIGSFIGGKLL
MTLGNRHTVGIGVLITAASGLILALFPASTNDIFFYLNSAFMGFGFGIIFTTTTVTVQDAVPRFQTGIATASNTLFRTVG
QTIGVAVFGTIFNSVLTSEFRAAAGSEVNRSNLNQLISPQTSGNVSADLVDPLRDILYSGLHMVFWVMFACCIVSYFIHF
ILPKKHISGEK
>Mature_491_residues
MEQTKVKAVTIAVFVATFMAAIEGTIVSTAMPTIVSQLDGIELMNWIFSVYLLTSAVTVPIYGKLSDLYGRKNIFVIATI
IFIIGSSLCGFAQNMEQLIIFRAIQGIGAGGILPSTMTIIADVYPFEKRAKVLGFMGSAWGIAGVFGPLVGGFLVDQLSW
HWIFFINVPIGILTILLILLYLREKVEHVKLPIDYLGASFFTVALLGLLFALQRAGESLNWTEPLVVILFAVSIVLFIAF
YFVEKRAKDPIMPFVLFKNPVVLIGNLIGFLISAFLIGINVYIPMWAQGMLGHGATIAGFMLAPLSVTWIIGSFIGGKLL
MTLGNRHTVGIGVLITAASGLILALFPASTNDIFFYLNSAFMGFGFGIIFTTTTVTVQDAVPRFQTGIATASNTLFRTVG
QTIGVAVFGTIFNSVLTSEFRAAAGSEVNRSNLNQLISPQTSGNVSADLVDPLRDILYSGLHMVFWVMFACCIVSYFIHF
ILPKKHISGEK

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=454, Percent_Identity=29.5154185022026, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1790195, Length=414, Percent_Identity=30.4347826086957, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=28.1173594132029, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87081983, Length=402, Percent_Identity=24.1293532338308, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788392, Length=406, Percent_Identity=27.3399014778325, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790146, Length=155, Percent_Identity=30.3225806451613, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788509, Length=170, Percent_Identity=28.8235294117647, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787291, Length=191, Percent_Identity=31.9371727748691, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=176, Percent_Identity=26.1363636363636, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=536, Percent_Identity=23.6940298507463, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319770, Length=428, Percent_Identity=27.3364485981308, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323735, Length=447, Percent_Identity=25.503355704698, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325455, Length=526, Percent_Identity=23.7642585551331, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319783, Length=459, Percent_Identity=23.3115468409586, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321663, Length=218, Percent_Identity=27.5229357798165, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324954, Length=395, Percent_Identity=23.7974683544304, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323520, Length=295, Percent_Identity=26.4406779661017, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323935, Length=307, Percent_Identity=26.3843648208469, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53340; Mature: 53340

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCHHCCCCC
>Mature Secondary Structure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HCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]