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The map label for this gene is ppnK

Identifier: 226223586

GI number: 226223586

Start: 1000780

End: 1001574

Strand: Direct

Name: ppnK

Synonym: Lm4b_00988

Alternate gene names: 226223586

Gene position: 1000780-1001574 (Clockwise)

Preceding gene: 226223585

Following gene: 226223587

Centisome position: 34.36

GC content: 38.11

Gene sequence:

>795_bases
ATGAAATATATGATTACTTCCAAAGGGGACGAAAAATCCGATTTGCTGCGACTAAATATGATCGCAGGTTTTGGGGAATA
TGACATGGAATATGATGACGTAGAGCCTGAAATTGTTATTTCTATTGGTGGAGATGGAACGTTTCTGTCTGCTTTTCATC
AATACGAGGAACGCTTGGATGAAATTGCCTTTATAGGGATTCATACTGGTCATCTAGGCTTTTATGCGGATTGGAGACCA
GCTGAGGCAAATAAATTAGTTAAGCTTGTAGCAAAAGGAGAATATCAGAAAGTTTCCTATCCTCTTCTCAAAACAACCGT
GAAATATGGGATTGGCAAAAAAGAAGCAACATATTTAGCGCTTAATGAATCAACTGTAAAAAGTTCAGGAGGCCCTTTTG
TAGTAGATGTTGTGATTAACGATATTCATTTTGAACGATTTCGTGGAGATGGCCTTTGTATGTCAACACCTAGTGGAACA
ACGGCTTACAATAAATCGCTAGGTGGGGCACTTATGCATCCTTCTATTGAAGCAATGCAGTTAACAGAGATGGCATCGAT
TAATAATCGAGTGTACCGGACAATCGGTAGCCCACTAGTTTTTCCGAAACATCATGTTGTTAGTTTACAACCGGTTAATG
ACAAGGACTTTCAAATTTCTGTAGATCATTTGAGCATTTTGCACCGGGATGTACAAGAAATTCGGTATGAAGTATCTGCA
AAAAAAATTCACTTTGCCAGATTTAAATCATTTCCATTTTGGCGTCGTGTACACGATTCGTTTATTGAAGATTAA

Upstream 100 bases:

>100_bases
TCTCAAAATAAAGACGTATCAAAGGATAAAAAAGCAGTGCATCAAGTAATGCCAAAGAAAAATAAGTAATTAGTTGTAGG
AATAGGGGCGAATAGAAATA

Downstream 100 bases:

>100_bases
GCTAGCAGGAGGAAGCATGTGTTTTTAGAATGGAAAGTCGAAACGGAAGAAGATGGACTCTTACTTCGCACGTTTTTGAA
GAGTAAACATATTTCGAAAC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1 [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDVEPEIVISIGGDGTFLSAFHQYEERLDEIAFIGIHTGHLGFYADWRP
AEANKLVKLVAKGEYQKVSYPLLKTTVKYGIGKKEATYLALNESTVKSSGGPFVVDVVINDIHFERFRGDGLCMSTPSGT
TAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSA
KKIHFARFKSFPFWRRVHDSFIED

Sequences:

>Translated_264_residues
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDVEPEIVISIGGDGTFLSAFHQYEERLDEIAFIGIHTGHLGFYADWRP
AEANKLVKLVAKGEYQKVSYPLLKTTVKYGIGKKEATYLALNESTVKSSGGPFVVDVVINDIHFERFRGDGLCMSTPSGT
TAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSA
KKIHFARFKSFPFWRRVHDSFIED
>Mature_264_residues
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDVEPEIVISIGGDGTFLSAFHQYEERLDEIAFIGIHTGHLGFYADWRP
AEANKLVKLVAKGEYQKVSYPLLKTTVKYGIGKKEATYLALNESTVKSSGGPFVVDVVINDIHFERFRGDGLCMSTPSGT
TAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSA
KKIHFARFKSFPFWRRVHDSFIED

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=189, Percent_Identity=30.1587301587302, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 29890; Mature: 29890

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDVEPEIVISIGGDGTFLSAFHQYEERLD
CEEEEECCCCCHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
EIAFIGIHTGHLGFYADWRPAEANKLVKLVAKGEYQKVSYPLLKTTVKYGIGKKEATYLA
HEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEE
LNESTVKSSGGPFVVDVVINDIHFERFRGDGLCMSTPSGTTAYNKSLGGALMHPSIEAMQ
EECCHHHCCCCCEEEEEEEECHHHHHCCCCCEEEECCCCCCHHHHCCCCEEECCCHHHHH
LTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSA
HHHHHHHCCHHHHHCCCCEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
KKIHFARFKSFPFWRRVHDSFIED
HEEEEEHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDDVEPEIVISIGGDGTFLSAFHQYEERLD
CEEEEECCCCCHHHEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
EIAFIGIHTGHLGFYADWRPAEANKLVKLVAKGEYQKVSYPLLKTTVKYGIGKKEATYLA
HEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEE
LNESTVKSSGGPFVVDVVINDIHFERFRGDGLCMSTPSGTTAYNKSLGGALMHPSIEAMQ
EECCHHHCCCCCEEEEEEEECHHHHHCCCCCEEEECCCCCCHHHHCCCCEEECCCHHHHH
LTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQISVDHLSILHRDVQEIRYEVSA
HHHHHHHCCHHHHHCCCCEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
KKIHFARFKSFPFWRRVHDSFIED
HEEEEEHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]