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The map label for this gene is comEC [H]

Identifier: 226223563

GI number: 226223563

Start: 981229

End: 982359

Strand: Direct

Name: comEC [H]

Synonym: Lm4b_00965

Alternate gene names: 226223563

Gene position: 981229-982359 (Clockwise)

Preceding gene: 226223562

Following gene: 226223564

Centisome position: 33.69

GC content: 38.2

Gene sequence:

>1131_bases
ATGACAAAGAAATTAGTAATCTGTATAAGTTTAATCGCGATATTTTTAAGCGGTTGTAGTTTTTCCGAACCATCAACGAG
TAAATCGAACATTACCGAAAAAACATTGAATGGTGCAAGTTTTACATTTTTTGATGTTGGCCAAGGGGATAGCACACTTA
TCCAAGCAGAAGATGGTACAACTATTTTGATTGATACAGGTCGCCAAGATGATGACCGTATTTTAACCTATTTAAAAGAA
GCGAATATAAAGAAAATAGATTTGCTGCTACTGACACACCCGCACGCAGACCATATCGGTAATGCTGATAAAGTAATCGC
TACATACAAACCGAAAGAAATTTGGATGGATGGCTTAACTTTTTCTAGTAGTATTTACGAGAAAGTAATTGACGCAGCAT
TAGCTTCAGATGCCAAATACAAAGAACCGAGACGCGGCGAAAAAGCGACTTTTGGTCCATTTAATCTAGAGGTTTTAAGC
CCTGATAAATTGGAAAATGACGCTAATAATGATTCTATCGCTGTAAAAATCACCTATAAAGATATTTCCGCTATTTTTAC
AGGTGACGCAGAAAAAGGTCGAGAAAGAGAAATGGTAGACAGCGGTGCCGATTTAGAAGCGGATATTTTAGATTTAGGGC
ACCACGGATCGTCCACATCCAACCAGCCATTTTTCCTAGATAAAGTAAAACCCCAAGTGGCTGTTTACTCTGCTGAAATG
GCCAATAGTTACGGACATCCTCATGTGGAAGTGCTGGAGTGGTTAAAAGAGCGTGATATAAAAACATATGGAACAGATGT
GAATGGAACGGTAATCATTGAAACAGACGGTAAAAAAATCCATGTGCAAACCGAAAAATCAGGTACACCAAAAGCAGGTA
GCAGCTATAATAAAGAAAATAGCACACAAAAAACAGAAGATGCAACATCAGAAGAATTACCAGACAGCATTAATTTAAAT
ACAGCTTCTAGTGAAGATTTACAATTATTACCACTAATTGGGCCAGCGCTTGCAGAAAAAATCATTGCCGAGCGACCATA
TCAGTCCATAGATGACTTGAAAAAAATTAACGGCATTGGCGATGGCATTGTGCGCCAAATCAAAGAACAAGGCATTGCCG
TAACAAAGTAG

Upstream 100 bases:

>100_bases
TGACCGCGTGTAATCAAATTGATTTCAGGCGGTTTTTGTTTTAAAAACTTTACTGTTTCGCCTTCCTTTGCTATTCTACT
AAGAGAGCAGGAGGAGAAAG

Downstream 100 bases:

>100_bases
GTGATTAGGGTGAAAAAAGCAATTTTAGATCGGATAGAAGATGGCAAAGCAGTTTTTTTATTAGAACCAGACCAAACAGA
ATGGCAAATTGACAAAGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTKKLVICISLIAIFLSGCSFSEPSTSKSNITEKTLNGASFTFFDVGQGDSTLIQAEDGTTILIDTGRQDDDRILTYLKE
ANIKKIDLLLLTHPHADHIGNADKVIATYKPKEIWMDGLTFSSSIYEKVIDAALASDAKYKEPRRGEKATFGPFNLEVLS
PDKLENDANNDSIAVKITYKDISAIFTGDAEKGREREMVDSGADLEADILDLGHHGSSTSNQPFFLDKVKPQVAVYSAEM
ANSYGHPHVEVLEWLKERDIKTYGTDVNGTVIIETDGKKIHVQTEKSGTPKAGSSYNKENSTQKTEDATSEELPDSINLN
TASSEDLQLLPLIGPALAEKIIAERPYQSIDDLKKINGIGDGIVRQIKEQGIAVTK

Sequences:

>Translated_376_residues
MTKKLVICISLIAIFLSGCSFSEPSTSKSNITEKTLNGASFTFFDVGQGDSTLIQAEDGTTILIDTGRQDDDRILTYLKE
ANIKKIDLLLLTHPHADHIGNADKVIATYKPKEIWMDGLTFSSSIYEKVIDAALASDAKYKEPRRGEKATFGPFNLEVLS
PDKLENDANNDSIAVKITYKDISAIFTGDAEKGREREMVDSGADLEADILDLGHHGSSTSNQPFFLDKVKPQVAVYSAEM
ANSYGHPHVEVLEWLKERDIKTYGTDVNGTVIIETDGKKIHVQTEKSGTPKAGSSYNKENSTQKTEDATSEELPDSINLN
TASSEDLQLLPLIGPALAEKIIAERPYQSIDDLKKINGIGDGIVRQIKEQGIAVTK
>Mature_375_residues
TKKLVICISLIAIFLSGCSFSEPSTSKSNITEKTLNGASFTFFDVGQGDSTLIQAEDGTTILIDTGRQDDDRILTYLKEA
NIKKIDLLLLTHPHADHIGNADKVIATYKPKEIWMDGLTFSSSIYEKVIDAALASDAKYKEPRRGEKATFGPFNLEVLSP
DKLENDANNDSIAVKITYKDISAIFTGDAEKGREREMVDSGADLEADILDLGHHGSSTSNQPFFLDKVKPQVAVYSAEMA
NSYGHPHVEVLEWLKERDIKTYGTDVNGTVIIETDGKKIHVQTEKSGTPKAGSSYNKENSTQKTEDATSEELPDSINLNT
ASSEDLQLLPLIGPALAEKIIAERPYQSIDDLKKINGIGDGIVRQIKEQGIAVTK

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 41156; Mature: 41024

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLVICISLIAIFLSGCSFSEPSTSKSNITEKTLNGASFTFFDVGQGDSTLIQAEDGT
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCEEEEECCCC
TILIDTGRQDDDRILTYLKEANIKKIDLLLLTHPHADHIGNADKVIATYKPKEIWMDGLT
EEEEECCCCCHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCCCEEEEECCHHHHCCCCC
FSSSIYEKVIDAALASDAKYKEPRRGEKATFGPFNLEVLSPDKLENDANNDSIAVKITYK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHCCCCCCCCEEEEEEEEE
DISAIFTGDAEKGREREMVDSGADLEADILDLGHHGSSTSNQPFFLDKVKPQVAVYSAEM
CEEEEEECCCCCCCHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCCEEEEEEHHH
ANSYGHPHVEVLEWLKERDIKTYGTDVNGTVIIETDGKKIHVQTEKSGTPKAGSSYNKEN
HHCCCCCHHHHHHHHHHCCCEEECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCC
STQKTEDATSEELPDSINLNTASSEDLQLLPLIGPALAEKIIAERPYQSIDDLKKINGIG
CCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEHHCHHHHHHHHHHCCHHHHHHHHHHCCCC
DGIVRQIKEQGIAVTK
HHHHHHHHHCCCEECC
>Mature Secondary Structure 
TKKLVICISLIAIFLSGCSFSEPSTSKSNITEKTLNGASFTFFDVGQGDSTLIQAEDGT
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCEEEEECCCC
TILIDTGRQDDDRILTYLKEANIKKIDLLLLTHPHADHIGNADKVIATYKPKEIWMDGLT
EEEEECCCCCHHHHHHHHHHCCCEEEEEEEEECCCHHHCCCCCCEEEEECCHHHHCCCCC
FSSSIYEKVIDAALASDAKYKEPRRGEKATFGPFNLEVLSPDKLENDANNDSIAVKITYK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHCCCCCCCCEEEEEEEEE
DISAIFTGDAEKGREREMVDSGADLEADILDLGHHGSSTSNQPFFLDKVKPQVAVYSAEM
CEEEEEECCCCCCCHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCCEEEEEEHHH
ANSYGHPHVEVLEWLKERDIKTYGTDVNGTVIIETDGKKIHVQTEKSGTPKAGSSYNKEN
HHCCCCCHHHHHHHHHHCCCEEECCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCC
STQKTEDATSEELPDSINLNTASSEDLQLLPLIGPALAEKIIAERPYQSIDDLKKINGIG
CCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEHHCHHHHHHHHHHCCHHHHHHHHHHCCCC
DGIVRQIKEQGIAVTK
HHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]