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The map label for this gene is yibK [C]

Identifier: 226223554

GI number: 226223554

Start: 974249

End: 974758

Strand: Direct

Name: yibK [C]

Synonym: Lm4b_00955

Alternate gene names: 226223554

Gene position: 974249-974758 (Clockwise)

Preceding gene: 226223553

Following gene: 226223555

Centisome position: 33.45

GC content: 36.67

Gene sequence:

>510_bases
ATGCCAAACCATATCGTACTATATCAACCAGAAATCCCAGCGAATACCGGAAATATCTCCAGAACATGTGCCGGGACGGA
TACTTACTTACATTTAATCCGTCCACTTGGTTTTTCGACAGATGATAAAATGTTAAAACGTGCTGGACTTGATTACTGGG
ATAACGTAAAACTTTCCTATTACGATTCTCTAGATGAATTCTTCGAGAAAAATGAAGGCGGAGAATTTTACTACATTACC
AAATTTGGTCGTCATGTTTATAGTGATATCGATTATAGTGACCCAAACAAAGATTATTTCTTCGTTTTTGGAAAAGAAAC
GACCGGATTGCCAGATGCGCTTTTACAAAAGAATGAAGAAAATTGTTTGCGTATTCCAATGACAGATCATATTCGTTCGT
TGAATTTATCGAACACAGCGGCAATTTTAGCTTATGAAGCGTTACGTCAGCAGAATTTTGGCGCACTTTTACAAGAACCG
AATTACGATCGAAAAATTTTTCAAGACTAA

Upstream 100 bases:

>100_bases
CCGAAAAAGCTGTCGAGCAAGCACTTCAAACCGAACAAGATGAACAAGTATTACAAGAATTAACAGCCATAACAAATTAA
TAGAAACCAGGTGAAAAGCA

Downstream 100 bases:

>100_bases
GGGGGAGTTAGGATGAATCAGGCGATAGATGCTATTCTAGGACATTACTCAGTGCGAAAATTTGAAGATAAGGACTTAAC
GGAAGAAGCGTTGACCTTAC

Product: CspR protein, rRNA methylase homolog

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MPNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYIT
KFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDALLQKNEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGALLQEP
NYDRKIFQD

Sequences:

>Translated_169_residues
MPNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYIT
KFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDALLQKNEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGALLQEP
NYDRKIFQD
>Mature_168_residues
PNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSYYDSLDEFFEKNEGGEFYYITK
FGRHVYSDIDYSDPNKDYFFVFGKETTGLPDALLQKNEENCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGALLQEPN
YDRKIFQD

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=42.6751592356688, Blast_Score=126, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19601; Mature: 19470

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSY
CCCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEE
YDSLDEFFEKNEGGEFYYITKFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDALLQKNEE
HHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCC
NCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGALLQEPNYDRKIFQD
CEEECCHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PNHIVLYQPEIPANTGNISRTCAGTDTYLHLIRPLGFSTDDKMLKRAGLDYWDNVKLSY
CCCEEEECCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEE
YDSLDEFFEKNEGGEFYYITKFGRHVYSDIDYSDPNKDYFFVFGKETTGLPDALLQKNEE
HHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCC
NCLRIPMTDHIRSLNLSNTAAILAYEALRQQNFGALLQEPNYDRKIFQD
CEEECCHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]