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The map label for this gene is lplA [H]

Identifier: 226223550

GI number: 226223550

Start: 968905

End: 969900

Strand: Direct

Name: lplA [H]

Synonym: Lm4b_00950

Alternate gene names: 226223550

Gene position: 968905-969900 (Clockwise)

Preceding gene: 226223549

Following gene: 226223553

Centisome position: 33.26

GC content: 35.74

Gene sequence:

>996_bases
ATGTACTTTATAGATAATAATAATGAGAAAGATCCTCGTATTAATTTAGCGGTGGAGGAATTTATTTTAACTGAATTAAA
TCTAGATGAGCCTGTGCTGTTATTTTATATTAATAAGCCATCGATTATCATTGGACGCAACCAAAATACAGTAGAAGAAA
TTGATACAGAGTATGTGGAGAAAAATGATGTCATCGTTGTGCGCAGACTTTCTGGCGGCGGCGCTGTTTATCATGATGAA
GGAAACTTAAATTTCAGTTTTATCACGGAAGATGATGGCGAGTCTTTCCATAATTTTGCGAAATTCACGCAACCAATCGT
GGAAGCGCTGAAACGTTTAGGCGTCAATGCGGAACTAAAAGGGCGTAACGACTTATTGATTGATGGTTTTAAAGTTTCTG
GAAATGCGCAATTTGCAACGAAAGGGAAAATGTTCTCACACGGGACATTAATGTATGATTTGAACCTAGATAATGTTGCT
GCATCGCTAAAACCACGTAAAGATAAAATCGAATCAAAAGGAATTAAGTCTGTTCGTAGTCGTGTAGCGAATATTTCTGA
TTTCATGGATCAAGAAATGACAACGGAAGAGTTTCGCGATTTGCTATTACTTTATATTTTCAATGTTGAAAAAGTAGAAG
ACGTAAAAGAATATAAATTAACTGCTGCAGATTGGGAAAAAATCCACGAAATCTCTGCTAAACGTTATGGTAATTGGGAC
TGGAACTACGGAAAATCTCCAAAATTCGATTTAACACGAACAAAACGTTTTCCAGTAGGAGCAGTCGATGTTCGCTTAAA
TGTACAAAAAGGGATTATTACAGATATCAAGGTTTTCGGTGACTTCTTCGGCGTAAAAAATGTGGCGGATATTGAGGAGA
AATTAGTCAATACAACTTATAAGCGCGAAGCATTGGACGAGGCTTTAGCAGATATAGACGTAAAAGAATACTTTGGTAAC
ATTACGAAAGATGAATTTTTAGATTTACTTTATTAA

Upstream 100 bases:

>100_bases
TTCTAAAACAAGAAGCAGCGGCTATTTACAAAGGAGAAATTGCACTCGCTTCAAAAGGATTCACGAAAACATTTTAATAT
AGAAGAAAAGGGGTAGGAGT

Downstream 100 bases:

>100_bases
AAAAATGGAAAGTACGCATCCCGCGTGCTTTCCATTTTTTTATTTGAGATTTTTTCCTGACATCCAATGTTCCAGCCGTT
TTCCAACAAACCATAAAATA

Product: lipoate protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MYFIDNNNEKDPRINLAVEEFILTELNLDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVEKNDVIVVRRLSGGGAVYHDE
GNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELKGRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVA
ASLKPRKDKIESKGIKSVRSRVANISDFMDQEMTTEEFRDLLLLYIFNVEKVEDVKEYKLTAADWEKIHEISAKRYGNWD
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITDIKVFGDFFGVKNVADIEEKLVNTTYKREALDEALADIDVKEYFGN
ITKDEFLDLLY

Sequences:

>Translated_331_residues
MYFIDNNNEKDPRINLAVEEFILTELNLDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVEKNDVIVVRRLSGGGAVYHDE
GNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELKGRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVA
ASLKPRKDKIESKGIKSVRSRVANISDFMDQEMTTEEFRDLLLLYIFNVEKVEDVKEYKLTAADWEKIHEISAKRYGNWD
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITDIKVFGDFFGVKNVADIEEKLVNTTYKREALDEALADIDVKEYFGN
ITKDEFLDLLY
>Mature_331_residues
MYFIDNNNEKDPRINLAVEEFILTELNLDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVEKNDVIVVRRLSGGGAVYHDE
GNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELKGRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVA
ASLKPRKDKIESKGIKSVRSRVANISDFMDQEMTTEEFRDLLLLYIFNVEKVEDVKEYKLTAADWEKIHEISAKRYGNWD
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITDIKVFGDFFGVKNVADIEEKLVNTTYKREALDEALADIDVKEYFGN
ITKDEFLDLLY

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=242, Percent_Identity=31.8181818181818, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI21729884, Length=242, Percent_Identity=31.8181818181818, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI21729882, Length=242, Percent_Identity=31.8181818181818, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI21729880, Length=242, Percent_Identity=31.8181818181818, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI21729878, Length=242, Percent_Identity=31.8181818181818, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1790846, Length=312, Percent_Identity=33.974358974359, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71981909, Length=255, Percent_Identity=35.6862745098039, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322415, Length=193, Percent_Identity=33.160621761658, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28573464, Length=283, Percent_Identity=26.1484098939929, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38003; Mature: 38003

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFIDNNNEKDPRINLAVEEFILTELNLDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVE
CEEECCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHC
KNDVIVVRRLSGGGAVYHDEGNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELK
CCCEEEEEEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVAASLKPRKDKIESKGIKSVRS
CCCCEEEEEEEECCCCEEEECCCEEECCEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHH
RVANISDFMDQEMTTEEFRDLLLLYIFNVEKVEDVKEYKLTAADWEKIHEISAKRYGNWD
HHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITDIKVFGDFFGVKNVADIEEKLVNTTY
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KREALDEALADIDVKEYFGNITKDEFLDLLY
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MYFIDNNNEKDPRINLAVEEFILTELNLDEPVLLFYINKPSIIIGRNQNTVEEIDTEYVE
CEEECCCCCCCCEEEEEHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHC
KNDVIVVRRLSGGGAVYHDEGNLNFSFITEDDGESFHNFAKFTQPIVEALKRLGVNAELK
CCCEEEEEEECCCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
GRNDLLIDGFKVSGNAQFATKGKMFSHGTLMYDLNLDNVAASLKPRKDKIESKGIKSVRS
CCCCEEEEEEEECCCCEEEECCCEEECCEEEEEECHHHHHHCCCCCHHHHHHHHHHHHHH
RVANISDFMDQEMTTEEFRDLLLLYIFNVEKVEDVKEYKLTAADWEKIHEISAKRYGNWD
HHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
WNYGKSPKFDLTRTKRFPVGAVDVRLNVQKGIITDIKVFGDFFGVKNVADIEEKLVNTTY
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KREALDEALADIDVKEYFGNITKDEFLDLLY
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]