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The map label for this gene is potD-A [H]

Identifier: 226223427

GI number: 226223427

Start: 837250

End: 838323

Strand: Direct

Name: potD-A [H]

Synonym: Lm4b_00825

Alternate gene names: 226223427

Gene position: 837250-838323 (Clockwise)

Preceding gene: 226223426

Following gene: 226223428

Centisome position: 28.74

GC content: 39.2

Gene sequence:

>1074_bases
ATGAAGCAACTGCTGAAAATTTTTGTGCCAGTCATTGTTGTCGCACTCCTAATGATGCTACTTGCAACAACGATGAACCG
CTCTGAGGGCTATGCTGGGAGCAATACGCTGACTATTTATAACTGGGGCGATTACATTGATCCATCGCTCATTACTAAAT
TTGAAAAAGAAACAGGCATCAAGGTCATTTACCAAACATTTGATTCTAATGAAGCGATGATGACGAAAATTGAGCAAGGT
GGAACGACCTTTGATATTGCGGTGCCAAGTGATTACGCGATTAGCAAAATGAAAGAAGAAAATTTGTTGATCCCACTTGA
TCATTCCAAATTACCAAATGAAAAATACCTTGATCCGCGTTTTATGGATTTATCGTTTGATGATGATAATAAATATTCGA
TGCCTTATTTCTGGGGAACGCTTGGCATTATTTATAATAAAGAAATGTTCCCGGATAAAAATTTCGATACGTGGAATGCG
CTATTTGATCCCGAATTGAAAAACCAAATTTTGCTGATTGATGGGGCGCGTGAAGTGATGGGGCTTGGGCTGAATAGTCT
CGGTTACTCACTGAACGATACGAATAAAGCTCACCTACAAGCTGCCAGAGACAAGCTAGAAACGATGACACCGAATGTTA
AAGCAATTGTTGGCGATGAGATTAAACTTCTCATGGCGGACAATGAGGCGGGTGTCGCGGTTACTTTCTCCGGAGAAGCG
GCGGAAATGTTAAGTGAAAATGAAGATTTAGAATACGTAATTCCAAAAGACGGCTCCAATTTATGGTTCGACAACATGGT
CATCCCAAAAACGGCGAAAAATGTCGATGGCGCGCATAAATTTATTAACTTCATGCTAAAACCAGAGAATGCGGCAATTA
ACGCTGAATATGTTGGCTATGCAACACCAAATGCAAAAGCTGTCGAATTGTTACCAAAAGAAATTTCGAGTGATGAACGT
TTTTATCCGGATATGGATGAACTGAATAATTTAGAAGTATATGATAACCTCGGTAAACGAATGCTGTCGTATTATAATGA
ATTATTTTTGGAGTTTAAGATGTACCGGAAATAA

Upstream 100 bases:

>100_bases
CGACACTGATTTTCTTATTCACGATTATTCTCGTGATCGGTTATTACTTTATCAACCAACGTAATACAAGTAAAAATATT
CGGACGGGGGCGACGAAAGA

Downstream 100 bases:

>100_bases
AGCCACCAGTATCTTCACGTGAGTTCACACCAAAGTCAAAACACGAACATTAAGCATATAACTTGTGGAATAGATCAGCT
ATCCGCTAAAATAATGTTAC

Product: spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGIKVIYQTFDSNEAMMTKIEQG
GTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPRFMDLSFDDDNKYSMPYFWGTLGIIYNKEMFPDKNFDTWNA
LFDPELKNQILLIDGAREVMGLGLNSLGYSLNDTNKAHLQAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
AEMLSENEDLEYVIPKDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGYATPNAKAVELLPKEISSDER
FYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK

Sequences:

>Translated_357_residues
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGIKVIYQTFDSNEAMMTKIEQG
GTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPRFMDLSFDDDNKYSMPYFWGTLGIIYNKEMFPDKNFDTWNA
LFDPELKNQILLIDGAREVMGLGLNSLGYSLNDTNKAHLQAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
AEMLSENEDLEYVIPKDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGYATPNAKAVELLPKEISSDER
FYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK
>Mature_357_residues
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGIKVIYQTFDSNEAMMTKIEQG
GTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPRFMDLSFDDDNKYSMPYFWGTLGIIYNKEMFPDKNFDTWNA
LFDPELKNQILLIDGAREVMGLGLNSLGYSLNDTNKAHLQAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
AEMLSENEDLEYVIPKDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGYATPNAKAVELLPKEISSDER
FYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=38.8888888888889, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1787078, Length=357, Percent_Identity=34.1736694677871, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40650; Mature: 40650

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCE
KVIYQTFDSNEAMMTKIEQGGTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPR
EEEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCE
FMDLSFDDDNKYSMPYFWGTLGIIYNKEMFPDKNFDTWNALFDPELKNQILLIDGAREVM
EEEEEECCCCCEECCCHHEEEEEEECCCCCCCCCCCCCCCEECCCCCCEEEEEECCHHHH
GLGLNSLGYSLNDTNKAHLQAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
HCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEEECCHH
AEMLSENEDLEYVIPKDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGY
HHHHCCCCCCEEEECCCCCCEEECCEECCCCCCCCCHHHHHHHEEECCCCCEECEEEEEE
ATPNAKAVELLPKEISSDERFYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK
ECCCCHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQLLKIFVPVIVVALLMMLLATTMNRSEGYAGSNTLTIYNWGDYIDPSLITKFEKETGI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCE
KVIYQTFDSNEAMMTKIEQGGTTFDIAVPSDYAISKMKEENLLIPLDHSKLPNEKYLDPR
EEEEEECCCCCHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCE
FMDLSFDDDNKYSMPYFWGTLGIIYNKEMFPDKNFDTWNALFDPELKNQILLIDGAREVM
EEEEEECCCCCEECCCHHEEEEEEECCCCCCCCCCCCCCCEECCCCCCEEEEEECCHHHH
GLGLNSLGYSLNDTNKAHLQAARDKLETMTPNVKAIVGDEIKLLMADNEAGVAVTFSGEA
HCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCCCEEEEECCHH
AEMLSENEDLEYVIPKDGSNLWFDNMVIPKTAKNVDGAHKFINFMLKPENAAINAEYVGY
HHHHCCCCCCEEEECCCCCCEEECCEECCCCCCCCCHHHHHHHEEECCCCCEECEEEEEE
ATPNAKAVELLPKEISSDERFYPDMDELNNLEVYDNLGKRMLSYYNELFLEFKMYRK
ECCCCHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]