Click here to switch to the map view.

The map label for this gene is lysP [H]

Identifier: 226223416

GI number: 226223416

Start: 826727

End: 828187

Strand: Reverse

Name: lysP [H]

Synonym: Lm4b_00814

Alternate gene names: 226223416

Gene position: 828187-826727 (Counterclockwise)

Preceding gene: 226223417

Following gene: 226223414

Centisome position: 28.43

GC content: 39.7

Gene sequence:

>1461_bases
GTGAAGAAAGAAACACATGGCGAGATTCGTCGTGACTTAAAAACAAGGCATTTGTCCATGATTGCCATTGGTGGTTCAAT
CGGGACGGGATTATTTTTAGCAAGTGGTAATGCGATTCATACAGCAGGACCAGGTGGCGCTTTAGTTGCTTATATAGCAA
TCGGGATTATGGTGTACTTTTTAATGACAAGTTTAGGCGAAATGGCAACTTACATGCCTGTATCTGGTTCATTTAGTACC
TATGCTAGTCGATTTGTTGACCCAGCTTTCGGTTTTGCGCTTGGTTGGAATTATTGGTTTAACTGGGCCATTACACTTGC
AGTTGATATTTCCACTGCTGCAATTATCGTTCAATTTTGGCTACCGAACACCCCCGCTTGGCTATGGAGCGCGATTTTCT
TAATTTTGATTTTCGGCTTAAACGCGCTTTCGGTAAAAGCATACGGAGAATCAGAGTATTGGTTTTCAATTATTAAAGTG
GCCACGGTTATTATTTTCCTTATCGTCGGCGTGCTTACTATTGTCGGAATTCTTGGCGGCGAAGTTATTGGCTTTTCTAA
CTTTACTGCTGGCGATGCTCCGTTTAAAGGTGGATTTTTTGCCATCTTAGGTACCTTCTTAATTGCTGGATTTTCTTTCC
AAGGGACTGAAATGGTTGGTATTGCAGCTGGTGAAAGTGCTACTCCAGAAACCAGCGTGCCAAAAGCGATTAAACAAGTA
TTCTGGCGGATTTTGCTATTTTATATTTTTGCGATTTTCATTATTGGGATGATTATTCCTTATACTAACCCGAATTTACT
TAGTGCCGAGGCAACAGATGTGGCAATTAGCCCATTTACACTCGTATTTGAAAAAGCTGGTCTTGCATTTGCGGCTTCCG
TGATGAATGCGGTTATCCTGACTTCTGTTTTATCTGCTGGTAATTCAGGTCTTTATGCTTCCACAAGAATGCTTTGGGCA
ATGGCTCGTGATAAAAAAGCACCTAAATTCTTGGGTAAAGTAAACCGTCGTGGTATTCCGATGGCAGCTTTAATTGTTAC
GACGATTGTTGGTGCAATGACATTCATTACAACGCTAACAGAAAACGGCACAGTGATTTATACGTGGTTACTTTCCGCCT
CTGGTTTAACCGGATTTATTGCTTGGGTCGGCATCGCTATCAGTCATTACCGTTTCCGAAAAGCATTTATCAAACAAGGA
CATGACTTAAGTGAACTAAAATATAAAGCAAAATTTTTCCCATTCGGACCTATTTTAGCACTAATTTTATGTATTTTAGT
TATTGTCGGTCAAGATTACGCGGCATTTTTGAAACCAGAATTTACGAATCCTGCTTGGTGGCAAAAAATCGGTATTTCTT
ATATCGGCCTCCCTATTTTCCTTGTTTTCTGGCTATCTTTTAAATTTACAAATAAAACAAAAGTTATTCCACTGGAAGAC
TGCAAATTTGATCAAAAATAA

Upstream 100 bases:

>100_bases
CTATCTGACAAAATGATGTTGGTAAAATACGTAACAGCCAATTTCTTGCAGATTGGCTGTTTTTGCGCACTTCCTCTGAT
AATAGAAAGGGAGATTAATA

Downstream 100 bases:

>100_bases
AAAAACATCTCTTGTTCATTCATTTTTACTTGAATGATACAGGAGATGTTTTTATTTACTCTTTTTCAGTAAGGTTTCGA
CTTTTTTATTTAGGCGAATT

Product: lysine-specific permease

Products: Proton [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKKETHGEIRRDLKTRHLSMIAIGGSIGTGLFLASGNAIHTAGPGGALVAYIAIGIMVYFLMTSLGEMATYMPVSGSFST
YASRFVDPAFGFALGWNYWFNWAITLAVDISTAAIIVQFWLPNTPAWLWSAIFLILIFGLNALSVKAYGESEYWFSIIKV
ATVIIFLIVGVLTIVGILGGEVIGFSNFTAGDAPFKGGFFAILGTFLIAGFSFQGTEMVGIAAGESATPETSVPKAIKQV
FWRILLFYIFAIFIIGMIIPYTNPNLLSAEATDVAISPFTLVFEKAGLAFAASVMNAVILTSVLSAGNSGLYASTRMLWA
MARDKKAPKFLGKVNRRGIPMAALIVTTIVGAMTFITTLTENGTVIYTWLLSASGLTGFIAWVGIAISHYRFRKAFIKQG
HDLSELKYKAKFFPFGPILALILCILVIVGQDYAAFLKPEFTNPAWWQKIGISYIGLPIFLVFWLSFKFTNKTKVIPLED
CKFDQK

Sequences:

>Translated_486_residues
MKKETHGEIRRDLKTRHLSMIAIGGSIGTGLFLASGNAIHTAGPGGALVAYIAIGIMVYFLMTSLGEMATYMPVSGSFST
YASRFVDPAFGFALGWNYWFNWAITLAVDISTAAIIVQFWLPNTPAWLWSAIFLILIFGLNALSVKAYGESEYWFSIIKV
ATVIIFLIVGVLTIVGILGGEVIGFSNFTAGDAPFKGGFFAILGTFLIAGFSFQGTEMVGIAAGESATPETSVPKAIKQV
FWRILLFYIFAIFIIGMIIPYTNPNLLSAEATDVAISPFTLVFEKAGLAFAASVMNAVILTSVLSAGNSGLYASTRMLWA
MARDKKAPKFLGKVNRRGIPMAALIVTTIVGAMTFITTLTENGTVIYTWLLSASGLTGFIAWVGIAISHYRFRKAFIKQG
HDLSELKYKAKFFPFGPILALILCILVIVGQDYAAFLKPEFTNPAWWQKIGISYIGLPIFLVFWLSFKFTNKTKVIPLED
CKFDQK
>Mature_486_residues
MKKETHGEIRRDLKTRHLSMIAIGGSIGTGLFLASGNAIHTAGPGGALVAYIAIGIMVYFLMTSLGEMATYMPVSGSFST
YASRFVDPAFGFALGWNYWFNWAITLAVDISTAAIIVQFWLPNTPAWLWSAIFLILIFGLNALSVKAYGESEYWFSIIKV
ATVIIFLIVGVLTIVGILGGEVIGFSNFTAGDAPFKGGFFAILGTFLIAGFSFQGTEMVGIAAGESATPETSVPKAIKQV
FWRILLFYIFAIFIIGMIIPYTNPNLLSAEATDVAISPFTLVFEKAGLAFAASVMNAVILTSVLSAGNSGLYASTRMLWA
MARDKKAPKFLGKVNRRGIPMAALIVTTIVGAMTFITTLTENGTVIYTWLLSASGLTGFIAWVGIAISHYRFRKAFIKQG
HDLSELKYKAKFFPFGPILALILCILVIVGQDYAAFLKPEFTNPAWWQKIGISYIGLPIFLVFWLSFKFTNKTKVIPLED
CKFDQK

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of lysine [H]

COG id: COG0833

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788480, Length=479, Percent_Identity=58.6638830897704, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI87081708, Length=459, Percent_Identity=38.1263616557734, Blast_Score=341, Evalue=7e-95,
Organism=Escherichia coli, GI48994972, Length=437, Percent_Identity=36.3844393592677, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1786789, Length=468, Percent_Identity=36.3247863247863, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI1786302, Length=463, Percent_Identity=35.6371490280778, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1789017, Length=409, Percent_Identity=36.1858190709046, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87081915, Length=413, Percent_Identity=36.319612590799, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1790653, Length=473, Percent_Identity=32.1353065539112, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1786602, Length=410, Percent_Identity=36.0975609756098, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87081869, Length=278, Percent_Identity=23.021582733813, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082023, Length=301, Percent_Identity=22.9235880398671, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320772, Length=491, Percent_Identity=38.6965376782077, Blast_Score=344, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6324061, Length=498, Percent_Identity=37.5502008032129, Blast_Score=331, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6322892, Length=493, Percent_Identity=38.9452332657201, Blast_Score=319, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6324990, Length=494, Percent_Identity=36.0323886639676, Blast_Score=318, Evalue=1e-87,
Organism=Saccharomyces cerevisiae, GI6324059, Length=498, Percent_Identity=36.5461847389558, Blast_Score=306, Evalue=6e-84,
Organism=Saccharomyces cerevisiae, GI6321629, Length=488, Percent_Identity=32.5819672131148, Blast_Score=282, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6324553, Length=434, Percent_Identity=37.0967741935484, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6324924, Length=496, Percent_Identity=32.6612903225806, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6319542, Length=489, Percent_Identity=32.1063394683027, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320717, Length=473, Percent_Identity=29.1754756871036, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6320251, Length=503, Percent_Identity=31.2127236580517, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6319824, Length=470, Percent_Identity=29.7872340425532, Blast_Score=249, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6321053, Length=488, Percent_Identity=32.172131147541, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322967, Length=435, Percent_Identity=33.5632183908046, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319543, Length=488, Percent_Identity=30.9426229508197, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324981, Length=470, Percent_Identity=32.7659574468085, Blast_Score=226, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6319608, Length=484, Percent_Identity=29.3388429752066, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6320364, Length=557, Percent_Identity=30.5206463195691, Blast_Score=197, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 53060; Mature: 53060

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKETHGEIRRDLKTRHLSMIAIGGSIGTGLFLASGNAIHTAGPGGALVAYIAIGIMVYF
CCCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHH
LMTSLGEMATYMPVSGSFSTYASRFVDPAFGFALGWNYWFNWAITLAVDISTAAIIVQFW
HHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEEHHHHEEEEEEE
LPNTPAWLWSAIFLILIFGLNALSVKAYGESEYWFSIIKVATVIIFLIVGVLTIVGILGG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVIGFSNFTAGDAPFKGGFFAILGTFLIAGFSFQGTEMVGIAAGESATPETSVPKAIKQV
CEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
FWRILLFYIFAIFIIGMIIPYTNPNLLSAEATDVAISPFTLVFEKAGLAFAASVMNAVIL
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECHHHHHHHHCCHHHHHHHHHHHHH
TSVLSAGNSGLYASTRMLWAMARDKKAPKFLGKVNRRGIPMAALIVTTIVGAMTFITTLT
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
ENGTVIYTWLLSASGLTGFIAWVGIAISHYRFRKAFIKQGHDLSELKYKAKFFPFGPILA
CCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
LILCILVIVGQDYAAFLKPEFTNPAWWQKIGISYIGLPIFLVFWLSFKFTNKTKVIPLED
HHHHHHHHHCCCHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHEECCCEEEEECCC
CKFDQK
CCCCCC
>Mature Secondary Structure
MKKETHGEIRRDLKTRHLSMIAIGGSIGTGLFLASGNAIHTAGPGGALVAYIAIGIMVYF
CCCCHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHH
LMTSLGEMATYMPVSGSFSTYASRFVDPAFGFALGWNYWFNWAITLAVDISTAAIIVQFW
HHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEEHHHHEEEEEEE
LPNTPAWLWSAIFLILIFGLNALSVKAYGESEYWFSIIKVATVIIFLIVGVLTIVGILGG
CCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EVIGFSNFTAGDAPFKGGFFAILGTFLIAGFSFQGTEMVGIAAGESATPETSVPKAIKQV
CEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
FWRILLFYIFAIFIIGMIIPYTNPNLLSAEATDVAISPFTLVFEKAGLAFAASVMNAVIL
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECHHHHHHHHCCHHHHHHHHHHHHH
TSVLSAGNSGLYASTRMLWAMARDKKAPKFLGKVNRRGIPMAALIVTTIVGAMTFITTLT
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
ENGTVIYTWLLSASGLTGFIAWVGIAISHYRFRKAFIKQGHDLSELKYKAKFFPFGPILA
CCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
LILCILVIVGQDYAAFLKPEFTNPAWWQKIGISYIGLPIFLVFWLSFKFTNKTKVIPLED
HHHHHHHHHCCCHHHHCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHHEECCCEEEEECCC
CKFDQK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-lysine [Periplasm] = Proton [Cytoplasm] + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1315732; 9278503; 7551055 [H]