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The map label for this gene is azoR2 [H]

Identifier: 226223403

GI number: 226223403

Start: 810327

End: 810962

Strand: Direct

Name: azoR2 [H]

Synonym: Lm4b_00801

Alternate gene names: 226223403

Gene position: 810327-810962 (Clockwise)

Preceding gene: 226223396

Following gene: 226223404

Centisome position: 27.82

GC content: 36.48

Gene sequence:

>636_bases
ATGTCAAAAGTACTATTTATTAAAGCGTCACCACTTCCAAATGAAGTATCAAGAAGTTCGCAAGTTGCCGAAACGTTTAT
GGCCGAATATAAAGCCAAAAATCCTTCTGATACAGTAGAAGAATTAGTTTTATATAATACAGAAGTACCACTATTAGATT
TAGAATTAATGACAGCTGGTAGAGAATTACAAGCTGGCAAAGCTTTCACTGATTTAGCGCCAGATGTACAAAAAAAATTA
AATGCTTATAATGCGCTTACGGAACAATTCTTAGCAGCAGACAAATATGTTTTTGTGTTCCCGCTTTGGAACCTTGGAAT
CCCGCCATTATTAAAAGCTTATATTGATACTTTTGTTATTGCTGGAAAATCTTTCCGTTATACTGAGCACGGTCCAGAAG
CACTTTTAAAAGATAAAAAAGCAATCTTAATCCATGGTAGTGGCGGTATTTATTCTGCTGGGCCAACAAGTTCATTTACT
CACGGAGAACCTTATTTACGTACTATTTTACAATTTATCGGTATTAACGTTGTACCATCAATCTTTGTAGAAGGAATTGA
TCACAATCCGAGCAAAGAAGCGGAAATCGTTGCAGCTGCTAAAGCGGTAGCGCAGGAAAGTGCTGCAGAATTCTAA

Upstream 100 bases:

>100_bases
GTGAAAATGTCAATGTTTATTTACCAAAAATCAAATTGACTAGCTACGCGGATAGTACTATTATATAGAAGGAACAAATA
AAATCGATGAGGTGTTCATA

Downstream 100 bases:

>100_bases
AAAGTTTGTGAAAAAATTATTTCTTGCTAATGATATTAAGACAGTATTATCAACTGTCTAGACCAATAAAAACACATAAA
AAGATTGCTAATCCCTTTAT

Product: acyl-carrier protein phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSKVLFIKASPLPNEVSRSSQVAETFMAEYKAKNPSDTVEELVLYNTEVPLLDLELMTAGRELQAGKAFTDLAPDVQKKL
NAYNALTEQFLAADKYVFVFPLWNLGIPPLLKAYIDTFVIAGKSFRYTEHGPEALLKDKKAILIHGSGGIYSAGPTSSFT
HGEPYLRTILQFIGINVVPSIFVEGIDHNPSKEAEIVAAAKAVAQESAAEF

Sequences:

>Translated_211_residues
MSKVLFIKASPLPNEVSRSSQVAETFMAEYKAKNPSDTVEELVLYNTEVPLLDLELMTAGRELQAGKAFTDLAPDVQKKL
NAYNALTEQFLAADKYVFVFPLWNLGIPPLLKAYIDTFVIAGKSFRYTEHGPEALLKDKKAILIHGSGGIYSAGPTSSFT
HGEPYLRTILQFIGINVVPSIFVEGIDHNPSKEAEIVAAAKAVAQESAAEF
>Mature_210_residues
SKVLFIKASPLPNEVSRSSQVAETFMAEYKAKNPSDTVEELVLYNTEVPLLDLELMTAGRELQAGKAFTDLAPDVQKKLN
AYNALTEQFLAADKYVFVFPLWNLGIPPLLKAYIDTFVIAGKSFRYTEHGPEALLKDKKAILIHGSGGIYSAGPTSSFTH
GEPYLRTILQFIGINVVPSIFVEGIDHNPSKEAEIVAAAKAVAQESAAEF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=202, Percent_Identity=39.6039603960396, Blast_Score=122, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 23066; Mature: 22934

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVLFIKASPLPNEVSRSSQVAETFMAEYKAKNPSDTVEELVLYNTEVPLLDLELMTAG
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHCC
RELQAGKAFTDLAPDVQKKLNAYNALTEQFLAADKYVFVFPLWNLGIPPLLKAYIDTFVI
CHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHH
AGKSFRYTEHGPEALLKDKKAILIHGSGGIYSAGPTSSFTHGEPYLRTILQFIGINVVPS
CCCCCCCCCCCHHHHHCCCCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHCCHHHHH
IFVEGIDHNPSKEAEIVAAAKAVAQESAAEF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKVLFIKASPLPNEVSRSSQVAETFMAEYKAKNPSDTVEELVLYNTEVPLLDLELMTAG
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHHHHCC
RELQAGKAFTDLAPDVQKKLNAYNALTEQFLAADKYVFVFPLWNLGIPPLLKAYIDTFVI
CHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHH
AGKSFRYTEHGPEALLKDKKAILIHGSGGIYSAGPTSSFTHGEPYLRTILQFIGINVVPS
CCCCCCCCCCCHHHHHCCCCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHCCHHHHH
IFVEGIDHNPSKEAEIVAAAKAVAQESAAEF
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]