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The map label for this gene is dagK [H]

Identifier: 226223392

GI number: 226223392

Start: 799823

End: 800752

Strand: Direct

Name: dagK [H]

Synonym: Lm4b_00790

Alternate gene names: 226223392

Gene position: 799823-800752 (Clockwise)

Preceding gene: 226223390

Following gene: 226223393

Centisome position: 27.46

GC content: 42.37

Gene sequence:

>930_bases
ATGGGGAAGGCACTATTGATTGTGAATCCATCATCAGGTAAAGAAAAAGGAAAAACTTATCAAGGAAAGACAGAAGAAGT
ACTAAAAAAACGTTATGATGAGGTGGAAGTTCGCCTGACTGAAAAAGCAGGGGATGCAACAGAATTTGCTTCCTGGGCTG
CTGAACAAGGCTTTGAAGCAGTAATTGCGATGGGTGGAGACGGGACTTTAAATGAAACCATAAACGGACTTGCAATCCAT
GAAAAACGACCTGACTTTGGCTTCATTCCACTTGGAACAGTCAATGATTTGGCTCGTTCGGTAGGTATACCATTAAAACC
CGAAAAAGCGATTCAAGCATTAGAAAAAGCTATTGCAGTGCCGATGGATATTGGCCGGATTGGCGACCAATACTTTATGA
ACGTCCTAGCTATCGGTATGATTGCGCAAGCTGTCGACCAAGTTAGCGTCGAACAAAAAACCAAATTTGGCTCCGTAGCA
TACTTTTTAGAAGGCCTGAAAGCATTTAACCGCAATGAATTACTTCATTTTAAAATCGAATATGACGAGGAAGTTTGGGA
AGGGGAAGCTGCGCTAGTTGTGGCTGGCTTAACTAATTCAGTTGGTGGGATGGAATCATGGGCGCCAGATGCAAAAATTG
ACGATGGCTACCTGCATATTATTATTCTAACGAAACTTGGCTTGCTCGATGCAGCTAACATGATCCCGCAATTAATTCGC
GGTAATTTAAAAAACAGTGACGGTGTAGTTTATATTAAAACCAAGAAACTTACCATTGATGCAAGTGGAGACGATTTAAG
CATCAACGTCGATGGCGACCCAGGTCCAGGTGTACCAGCAGAAATCGAAGTGCTTGGCAGTCACTTGAATATTCTTGCAC
CCAAAAAAAGCAGCAAGAAGCGCTTCGGCCCATTCATACTTAAAAAGTAA

Upstream 100 bases:

>100_bases
TGGTACAAATCCCCATTTCAAGGTAAACTAGAATAGAGAAGATGCCGATATTTTGTGTAAATTTATAAATAGAATTTTTT
GGCAAGAAAGGGTGAGTGCG

Downstream 100 bases:

>100_bases
CCTACAATAATTGTAGAAGAATAAGGAGATGAAAGCTTTATGGAAGAAGAATTAGAAGTCATTCATAAAGGTCTTGCCAA
TGCGAAAAATGGTTTTAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEAVIAMGGDGTLNETINGLAIH
EKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAVPMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVA
YFLEGLKAFNRNELLHFKIEYDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR
GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAPKKSSKKRFGPFILKK

Sequences:

>Translated_309_residues
MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEAVIAMGGDGTLNETINGLAIH
EKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAVPMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVA
YFLEGLKAFNRNELLHFKIEYDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR
GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAPKKSSKKRFGPFILKK
>Mature_308_residues
GKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEAVIAMGGDGTLNETINGLAIHE
KRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAVPMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAY
FLEGLKAFNRNELLHFKIEYDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIRG
NLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAPKKSSKKRFGPFILKK

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=302, Percent_Identity=29.1390728476821, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33424; Mature: 33293

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEA
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEE
VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAV
EEEECCCCCHHHCCCCEEEECCCCCCCEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
PMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKIE
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEE
YDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR
CCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHC
GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAPKKSSKK
CCCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHEEECCCCEEECCCCCCCH
RFGPFILKK
HCCCCEECC
>Mature Secondary Structure 
GKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEA
CCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEE
VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAV
EEEECCCCCHHHCCCCEEEECCCCCCCEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
PMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKIE
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEE
YDEEVWEGEAALVVAGLTNSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR
CCHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHC
GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAPKKSSKK
CCCCCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCHHHEEECCCCEEECCCCCCCH
RFGPFILKK
HCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA