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The map label for this gene is fliF

Identifier: 226223344

GI number: 226223344

Start: 752266

End: 753918

Strand: Direct

Name: fliF

Synonym: Lm4b_00741

Alternate gene names: 226223344

Gene position: 752266-753918 (Clockwise)

Preceding gene: 226223343

Following gene: 226223345

Centisome position: 25.83

GC content: 41.44

Gene sequence:

>1653_bases
ATGGCAAAAATAAAAACAATGTATTCGAAATTGAAGAATTGGCACAAAGGCGCGATTTTAGTCGGCCTTTTTGTCGTAGT
CACGGTGTTATTACTCTACATGAACACGCCCAAAACAGAAGTTACTCTTTATAAAAATCTATCCGAAACAAGCCAACAAC
AAGTCACCGATCAATTAGCCAAAATGGGCGTTGATTATACCGTCGACAAAAGTGGTAACATTTTAGTCGATGAAAAAGTG
GAGACACTCGTTCGTGACAAATTTGCGGACCTAGGTATTCCATACACAGGCCAAGATGGCAATGACATTCTACTAAACAG
CTCACTTGGAGCCAGTGAAGAAGATAAAAAAATGCAAGAAAAAGTTGGTACGAAAGTCAATTTAGAAAAAGAAATCGTTC
AAAGCTATGGAACAACCATCGATAGCGCCTCCGTGCAGCTAACTTTACCAGAATCAAGTTCGATTTTTGAAGAAGCAAGC
CAAAAAGGAACAGCCGCAGTAACACTCAAAACCAAAAACAATCAAACACTAACAAGCGAACAAGTTCTCGGCATTCAGCG
CACCGTCAGCGCAGCCGTACCAAATGTTGCTAGTGATGACGTAGCGATTATCGATACGAAAAATGGTGTTATCTCTGAAG
CAGACACATCCAAAGAAGAAGGCAGTTCTGCTTATAAAAATGAAGTCGATATCCAAAATGCGATTGGAAAAAATGTGAAA
ACAGATATCGAAGGGACACTTTCCAGCATTTTTGCCCTAGATAATTTCCGCGTGAATACAAATGTCTCGGTTAATTTTGA
CGAAATCAAACAAAATACGGAACATTATCCAAACGACGGAAAAGTACGTAGCAACCAAAAAGACACATCAACAGACACAT
CAAAAGGTTCCGCGAATACGACGGAATCAGGAACCGCCTCAAACGCTGACGTACCAAATTACACGGAACAAAATGGTGAT
GATACGAACACCTATACAAGCGAAAAATCAAGTGAAACAACGAATTATGAACTAGACTCCACCATCCAAGAAATCAAAAA
ACACCCAGCCCTAGCAAAGACAAATGTCGTTGTCTGGGTTGATCAAAATGCTTTAAATAAAAATGGTGTTGACATGGCCG
AATTCACAAAAGCAATTGGAGTTTCAGCTGGATTAACGCCTAATATGACAACCGAAGAAGCGGGCGCAGACGGAGAAGCA
GCAGCCGCACCAACATTCGAAGGTACTTTCCAAAATGGCGATGTTACCATCATGCCGATCCAGTTCTTAGACAACGCCAC
GCCAGCAGAAGAAGATACAACAGAAAAAGCAGCACCAGCAAGCAAAGCCTGGATTTGGTGGCTTGCAGGAAGTTTACTCT
TCGCCATCATTGCAGCTGGAATTATCGCCTACATTATCTTCCTTAAACGTAAAGAACAACTAGAAGAAGCATTAGAACCA
GAAGAAAAAGACTACATTCCAGCCGAAGAAGCAATCATCAACCCAGAAGAACATCCAGATTTCAACTTCCAAACAGATGC
ATTCGATTTATCAGAACCAGAATTAAAAGCCCGCAAAGAAAGCTTGAAAAATAAACTCGGTGAAATGGCCAAAGAAGACC
CAGGGCGCGCCGCAGCAGTCATCCAAAAATGGCTCAATGAAAGGCAGGAATAA

Upstream 100 bases:

>100_bases
AGCTACAAACAGCTTTTAAATATGCAAGTGTAGCGGTTAGACTAGTCGGGGGAGTTTTAGTCAATCGTCCACACGAGAAA
AATGGAGCGAAGTTTTTAAG

Downstream 100 bases:

>100_bases
CAATGGCAGAAACACTCAAAGAAACCTTAGAAGAAACAAATGCTGAACTAGAAACTGTCGAAGTAGAAGAAACGGAAGAA
AAAACAGCTACTTCAGCAGA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MAKIKTMYSKLKNWHKGAILVGLFVVVTVLLLYMNTPKTEVTLYKNLSETSQQQVTDQLAKMGVDYTVDKSGNILVDEKV
ETLVRDKFADLGIPYTGQDGNDILLNSSLGASEEDKKMQEKVGTKVNLEKEIVQSYGTTIDSASVQLTLPESSSIFEEAS
QKGTAAVTLKTKNNQTLTSEQVLGIQRTVSAAVPNVASDDVAIIDTKNGVISEADTSKEEGSSAYKNEVDIQNAIGKNVK
TDIEGTLSSIFALDNFRVNTNVSVNFDEIKQNTEHYPNDGKVRSNQKDTSTDTSKGSANTTESGTASNADVPNYTEQNGD
DTNTYTSEKSSETTNYELDSTIQEIKKHPALAKTNVVVWVDQNALNKNGVDMAEFTKAIGVSAGLTPNMTTEEAGADGEA
AAAPTFEGTFQNGDVTIMPIQFLDNATPAEEDTTEKAAPASKAWIWWLAGSLLFAIIAAGIIAYIIFLKRKEQLEEALEP
EEKDYIPAEEAIINPEEHPDFNFQTDAFDLSEPELKARKESLKNKLGEMAKEDPGRAAAVIQKWLNERQE

Sequences:

>Translated_550_residues
MAKIKTMYSKLKNWHKGAILVGLFVVVTVLLLYMNTPKTEVTLYKNLSETSQQQVTDQLAKMGVDYTVDKSGNILVDEKV
ETLVRDKFADLGIPYTGQDGNDILLNSSLGASEEDKKMQEKVGTKVNLEKEIVQSYGTTIDSASVQLTLPESSSIFEEAS
QKGTAAVTLKTKNNQTLTSEQVLGIQRTVSAAVPNVASDDVAIIDTKNGVISEADTSKEEGSSAYKNEVDIQNAIGKNVK
TDIEGTLSSIFALDNFRVNTNVSVNFDEIKQNTEHYPNDGKVRSNQKDTSTDTSKGSANTTESGTASNADVPNYTEQNGD
DTNTYTSEKSSETTNYELDSTIQEIKKHPALAKTNVVVWVDQNALNKNGVDMAEFTKAIGVSAGLTPNMTTEEAGADGEA
AAAPTFEGTFQNGDVTIMPIQFLDNATPAEEDTTEKAAPASKAWIWWLAGSLLFAIIAAGIIAYIIFLKRKEQLEEALEP
EEKDYIPAEEAIINPEEHPDFNFQTDAFDLSEPELKARKESLKNKLGEMAKEDPGRAAAVIQKWLNERQE
>Mature_549_residues
AKIKTMYSKLKNWHKGAILVGLFVVVTVLLLYMNTPKTEVTLYKNLSETSQQQVTDQLAKMGVDYTVDKSGNILVDEKVE
TLVRDKFADLGIPYTGQDGNDILLNSSLGASEEDKKMQEKVGTKVNLEKEIVQSYGTTIDSASVQLTLPESSSIFEEASQ
KGTAAVTLKTKNNQTLTSEQVLGIQRTVSAAVPNVASDDVAIIDTKNGVISEADTSKEEGSSAYKNEVDIQNAIGKNVKT
DIEGTLSSIFALDNFRVNTNVSVNFDEIKQNTEHYPNDGKVRSNQKDTSTDTSKGSANTTESGTASNADVPNYTEQNGDD
TNTYTSEKSSETTNYELDSTIQEIKKHPALAKTNVVVWVDQNALNKNGVDMAEFTKAIGVSAGLTPNMTTEEAGADGEAA
AAPTFEGTFQNGDVTIMPIQFLDNATPAEEDTTEKAAPASKAWIWWLAGSLLFAIIAAGIIAYIIFLKRKEQLEEALEPE
EKDYIPAEEAIINPEEHPDFNFQTDAFDLSEPELKARKESLKNKLGEMAKEDPGRAAAVIQKWLNERQE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=345, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60030; Mature: 59899

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKTMYSKLKNWHKGAILVGLFVVVTVLLLYMNTPKTEVTLYKNLSETSQQQVTDQLA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
KMGVDYTVDKSGNILVDEKVETLVRDKFADLGIPYTGQDGNDILLNSSLGASEEDKKMQE
HCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
KVGTKVNLEKEIVQSYGTTIDSASVQLTLPESSSIFEEASQKGTAAVTLKTKNNQTLTSE
HCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCHH
QVLGIQRTVSAAVPNVASDDVAIIDTKNGVISEADTSKEEGSSAYKNEVDIQNAIGKNVK
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHCCCHHHHCCCHHHHHHCCCCC
TDIEGTLSSIFALDNFRVNTNVSVNFDEIKQNTEHYPNDGKVRSNQKDTSTDTSKGSANT
CHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
TESGTASNADVPNYTEQNGDDTNTYTSEKSSETTNYELDSTIQEIKKHPALAKTNVVVWV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCEEEEE
DQNALNKNGVDMAEFTKAIGVSAGLTPNMTTEEAGADGEAAAAPTFEGTFQNGDVTIMPI
ECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEE
QFLDNATPAEEDTTEKAAPASKAWIWWLAGSLLFAIIAAGIIAYIIFLKRKEQLEEALEP
HCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEKDYIPAEEAIINPEEHPDFNFQTDAFDLSEPELKARKESLKNKLGEMAKEDPGRAAAV
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IQKWLNERQE
HHHHHHHCCC
>Mature Secondary Structure 
AKIKTMYSKLKNWHKGAILVGLFVVVTVLLLYMNTPKTEVTLYKNLSETSQQQVTDQLA
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHH
KMGVDYTVDKSGNILVDEKVETLVRDKFADLGIPYTGQDGNDILLNSSLGASEEDKKMQE
HCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
KVGTKVNLEKEIVQSYGTTIDSASVQLTLPESSSIFEEASQKGTAAVTLKTKNNQTLTSE
HCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCHH
QVLGIQRTVSAAVPNVASDDVAIIDTKNGVISEADTSKEEGSSAYKNEVDIQNAIGKNVK
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHCCCHHHHCCCHHHHHHCCCCC
TDIEGTLSSIFALDNFRVNTNVSVNFDEIKQNTEHYPNDGKVRSNQKDTSTDTSKGSANT
CHHHHHHHHHHHHCCEEECCCEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
TESGTASNADVPNYTEQNGDDTNTYTSEKSSETTNYELDSTIQEIKKHPALAKTNVVVWV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCEEEEE
DQNALNKNGVDMAEFTKAIGVSAGLTPNMTTEEAGADGEAAAAPTFEGTFQNGDVTIMPI
ECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCEEEEEE
QFLDNATPAEEDTTEKAAPASKAWIWWLAGSLLFAIIAAGIIAYIIFLKRKEQLEEALEP
HCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEKDYIPAEEAIINPEEHPDFNFQTDAFDLSEPELKARKESLKNKLGEMAKEDPGRAAAV
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IQKWLNERQE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]