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The map label for this gene is ychM [C]

Identifier: 226223154

GI number: 226223154

Start: 563657

End: 565318

Strand: Reverse

Name: ychM [C]

Synonym: Lm4b_00551

Alternate gene names: 226223154

Gene position: 565318-563657 (Counterclockwise)

Preceding gene: 226223155

Following gene: 226223151

Centisome position: 19.41

GC content: 39.83

Gene sequence:

>1662_bases
ATGTTTAAACACATCTTGCTTTCACTAAATGGGTACAAAATATCCTATTTACGAAATGATGTCATTTCAGGCGTTGGTGT
TGCTGCACTGACGATTCCAGTTGCAATGGGATATGCACAAGTAGCAGGACTTCCACCCATTTATGGATTATATGCATCAT
TTTTACCGGTTATTGCGTATGTTATTTTTGCCAGTTCACCACAACTTGTTTTCGGAATTGATGCAACGGCAAGTGCCATC
ACAGGATCCATCATTTTAGGAACAGCTGGACTTGCTGCCGGATCTAAAGAAGCTATCGCGCTCGCCCCAATTCTCGCATT
CTTTTGTGCAATATTTTTAGTTCTTTTCTCCGTATTAAAATTAGGACGTTTCGCAAAATACATATCAGCCCCGGTTCTTA
GTGGATTCATTTCCGGACTTAGTGTCTCCATCATTATGGGGCAAATTCCTAAGATAATGGGCCTAAAAGAAAGCGGAGAT
AGTTTCTTCTCTAGTCTAGGCATTATTTTCGGACAGTTTTTCCAGTCCAATTGGATTTCATTTGCCATGGGCGTGGTTAC
AGTCATTATTGTTATCACTTGTAAAAAAGTTATACCAAAAATCCCTATGTCTCTTGTAGTTTTAGTACTTGGGACAATGG
CTGCTTATTTCTTTAAATTAGATCAATATAATGTTGATATTGTAGGTAAAATTCCAGTTGGCTTCCCTTCACTAGCGCTA
CCCGATTTTGGTGCAAGTTCGTGGGCACTCGCGATTGGCGGCGGTTTAGTATGTGCCATCGCAACTTTTGCCGGTTCGCT
TTTACCAAGTGAAAGTTTTGCTATGAGGAACAAATATACGATTGATGATAATCGTGAGTTATTTGCTTATGGGATTTCTA
ATTTCGTCGCTGCGTTTTCTGGTTGTTCCCCAGCAAGTGCCAGCGTATCCAGAACTGCTGCTAACGAACAATTTCGTGGT
AAAACGCAAATGGTTTCTATCGTTGCTGCGACCATTATTGCGCTAATCGTTGCCTTCTTAAGTGGCTTACTTTACTATAT
GCCGCAACCAGTTCTTTCAGGTATCGTATTTGCTGCCCTTGTAGGTATTATTGATGTAGATGTGTTAAAAGGTTTATTCA
AAGTTTCTCGTCGTGAAGCAACTGTTTGGATTGTTGCAGCACTTGGTACCCTTTTAGTTGGCGTTATTTTCGGGGTACTA
CTTGGGATTTTCCTATCATTCATTAACGTAGTAAGTCGTTCGATGAAATCACCAATTGCCATTCTTGGAGTGATCGAAGG
ACGCCACGGTTATTTCGATTTAAAACGTAAACCAGAAGCAAAACCAATTCCAAATGTTGTCATTTATCGTTATAGCGCTT
CTCTTTTCTTCGGGAATTTCAACAAATTCGCAGACGGATTAAAAGAAGCAGTTCAAGATGATACAAAACTAGTCATTTTT
GAAGCGAGTGCGATTATTAACATTGATACAACCGCAACCGAATCTATGAAAGACTTGCTCAAATGGCTTGATGACAAAGG
CATTGAATATTACTTCGCTGACCTAATTGATCATTTGAAAACCAGCTTTAGAAAACATGATCTTGGCTATATTATCGATA
ACGGCTACACGAAGAAAACCGTGGAAGACGCGCTCGAGGCTTATTATGCCGAACATAAATAA

Upstream 100 bases:

>100_bases
GATTGGTATAACAAAGAACGGGGTAAATAGTATAAGATTTCATTAATATGTATTTATGATGTAAAATAAAGAATAAAAAT
ACATTTCAAGGGAGGAACTT

Downstream 100 bases:

>100_bases
CAAAACGCTTAGCCGGGCCTAAACTCGACTAAGCGTTTTTCATTTTTACAAAAGCGCAAAAATCCGCGGACTAATTAACC
GCACAAACGGTGACACGATA

Product: sulfate transporter

Products: Proton [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MFKHILLSLNGYKISYLRNDVISGVGVAALTIPVAMGYAQVAGLPPIYGLYASFLPVIAYVIFASSPQLVFGIDATASAI
TGSIILGTAGLAAGSKEAIALAPILAFFCAIFLVLFSVLKLGRFAKYISAPVLSGFISGLSVSIIMGQIPKIMGLKESGD
SFFSSLGIIFGQFFQSNWISFAMGVVTVIIVITCKKVIPKIPMSLVVLVLGTMAAYFFKLDQYNVDIVGKIPVGFPSLAL
PDFGASSWALAIGGGLVCAIATFAGSLLPSESFAMRNKYTIDDNRELFAYGISNFVAAFSGCSPASASVSRTAANEQFRG
KTQMVSIVAATIIALIVAFLSGLLYYMPQPVLSGIVFAALVGIIDVDVLKGLFKVSRREATVWIVAALGTLLVGVIFGVL
LGIFLSFINVVSRSMKSPIAILGVIEGRHGYFDLKRKPEAKPIPNVVIYRYSASLFFGNFNKFADGLKEAVQDDTKLVIF
EASAIINIDTTATESMKDLLKWLDDKGIEYYFADLIDHLKTSFRKHDLGYIIDNGYTKKTVEDALEAYYAEHK

Sequences:

>Translated_553_residues
MFKHILLSLNGYKISYLRNDVISGVGVAALTIPVAMGYAQVAGLPPIYGLYASFLPVIAYVIFASSPQLVFGIDATASAI
TGSIILGTAGLAAGSKEAIALAPILAFFCAIFLVLFSVLKLGRFAKYISAPVLSGFISGLSVSIIMGQIPKIMGLKESGD
SFFSSLGIIFGQFFQSNWISFAMGVVTVIIVITCKKVIPKIPMSLVVLVLGTMAAYFFKLDQYNVDIVGKIPVGFPSLAL
PDFGASSWALAIGGGLVCAIATFAGSLLPSESFAMRNKYTIDDNRELFAYGISNFVAAFSGCSPASASVSRTAANEQFRG
KTQMVSIVAATIIALIVAFLSGLLYYMPQPVLSGIVFAALVGIIDVDVLKGLFKVSRREATVWIVAALGTLLVGVIFGVL
LGIFLSFINVVSRSMKSPIAILGVIEGRHGYFDLKRKPEAKPIPNVVIYRYSASLFFGNFNKFADGLKEAVQDDTKLVIF
EASAIINIDTTATESMKDLLKWLDDKGIEYYFADLIDHLKTSFRKHDLGYIIDNGYTKKTVEDALEAYYAEHK
>Mature_553_residues
MFKHILLSLNGYKISYLRNDVISGVGVAALTIPVAMGYAQVAGLPPIYGLYASFLPVIAYVIFASSPQLVFGIDATASAI
TGSIILGTAGLAAGSKEAIALAPILAFFCAIFLVLFSVLKLGRFAKYISAPVLSGFISGLSVSIIMGQIPKIMGLKESGD
SFFSSLGIIFGQFFQSNWISFAMGVVTVIIVITCKKVIPKIPMSLVVLVLGTMAAYFFKLDQYNVDIVGKIPVGFPSLAL
PDFGASSWALAIGGGLVCAIATFAGSLLPSESFAMRNKYTIDDNRELFAYGISNFVAAFSGCSPASASVSRTAANEQFRG
KTQMVSIVAATIIALIVAFLSGLLYYMPQPVLSGIVFAALVGIIDVDVLKGLFKVSRREATVWIVAALGTLLVGVIFGVL
LGIFLSFINVVSRSMKSPIAILGVIEGRHGYFDLKRKPEAKPIPNVVIYRYSASLFFGNFNKFADGLKEAVQDDTKLVIF
EASAIINIDTTATESMKDLLKWLDDKGIEYYFADLIDHLKTSFRKHDLGYIIDNGYTKKTVEDALEAYYAEHK

Specific function: Expression in E.coli induces sulfate uptake during early-to mid-log phase growth. Uptake is maximal at pH 6.0, is sulfate-specific, requires E.coli CysA and the transmembrane segment but not the STAS domain of the protein [H]

COG id: COG0659

COG function: function code P; Sulfate permease and related transporters (MFS superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 STAS domain [H]

Homologues:

Organism=Homo sapiens, GI94721259, Length=477, Percent_Identity=27.2536687631027, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI94721253, Length=477, Percent_Identity=27.2536687631027, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI94721255, Length=477, Percent_Identity=27.2536687631027, Blast_Score=184, Evalue=3e-46,
Organism=Homo sapiens, GI94721257, Length=477, Percent_Identity=27.2536687631027, Blast_Score=184, Evalue=3e-46,
Organism=Homo sapiens, GI45827800, Length=499, Percent_Identity=26.0521042084168, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI39752683, Length=499, Percent_Identity=26.0521042084168, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI4505697, Length=534, Percent_Identity=27.3408239700375, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI100913030, Length=612, Percent_Identity=25.3267973856209, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI269784651, Length=498, Percent_Identity=24.0963855421687, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI45827802, Length=444, Percent_Identity=26.3513513513513, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI47131207, Length=495, Percent_Identity=26.4646464646465, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI20336272, Length=495, Percent_Identity=26.4646464646465, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI16306483, Length=490, Percent_Identity=25.1020408163265, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI20336282, Length=490, Percent_Identity=25.1020408163265, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI262206105, Length=547, Percent_Identity=24.1316270566728, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI262206075, Length=547, Percent_Identity=24.1316270566728, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI262206069, Length=547, Percent_Identity=24.1316270566728, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI262206063, Length=547, Percent_Identity=24.1316270566728, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4557535, Length=519, Percent_Identity=23.6994219653179, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI16418413, Length=497, Percent_Identity=25.1509054325956, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI217272867, Length=498, Percent_Identity=25.1004016064257, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI65506789, Length=376, Percent_Identity=26.8617021276596, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI16418457, Length=479, Percent_Identity=21.5031315240083, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI301601599, Length=479, Percent_Identity=21.5031315240083, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI45827804, Length=264, Percent_Identity=26.1363636363636, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI301601602, Length=184, Percent_Identity=23.3695652173913, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI87081859, Length=325, Percent_Identity=24.6153846153846, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17551690, Length=599, Percent_Identity=26.0434056761269, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI86565215, Length=564, Percent_Identity=26.063829787234, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17566848, Length=547, Percent_Identity=26.1425959780622, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI193203292, Length=638, Percent_Identity=22.7272727272727, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17562578, Length=520, Percent_Identity=26.9230769230769, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI86564196, Length=490, Percent_Identity=25.9183673469388, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI86565213, Length=254, Percent_Identity=29.9212598425197, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI86564876, Length=460, Percent_Identity=21.304347826087, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI86565209, Length=246, Percent_Identity=28.8617886178862, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI86565211, Length=254, Percent_Identity=27.9527559055118, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6325260, Length=574, Percent_Identity=23.1707317073171, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319771, Length=561, Percent_Identity=20.6773618538324, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6323121, Length=153, Percent_Identity=33.9869281045752, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24666186, Length=527, Percent_Identity=26.1859582542694, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24649801, Length=577, Percent_Identity=22.1837088388215, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI85815873, Length=431, Percent_Identity=24.3619489559165, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19922482, Length=568, Percent_Identity=23.5915492957746, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24663084, Length=537, Percent_Identity=25.512104283054, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21357695, Length=537, Percent_Identity=25.512104283054, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21358229, Length=461, Percent_Identity=24.9457700650759, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24647160, Length=428, Percent_Identity=23.5981308411215, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21355087, Length=428, Percent_Identity=23.5981308411215, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24651449, Length=468, Percent_Identity=22.4358974358974, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21358633, Length=524, Percent_Identity=21.7557251908397, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002645
- InterPro:   IPR001902
- InterPro:   IPR011547 [H]

Pfam domain/function: PF01740 STAS; PF00916 Sulfate_transp [H]

EC number: NA

Molecular weight: Translated: 59466; Mature: 59466

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS50801 STAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKHILLSLNGYKISYLRNDVISGVGVAALTIPVAMGYAQVAGLPPIYGLYASFLPVIAY
CCCEEEEEECCEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VIFASSPQLVFGIDATASAITGSIILGTAGLAAGSKEAIALAPILAFFCAIFLVLFSVLK
HHHCCCCCEEEEECCHHHHHHCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGRFAKYISAPVLSGFISGLSVSIIMGQIPKIMGLKESGDSFFSSLGIIFGQFFQSNWIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FAMGVVTVIIVITCKKVIPKIPMSLVVLVLGTMAAYFFKLDQYNVDIVGKIPVGFPSLAL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PDFGASSWALAIGGGLVCAIATFAGSLLPSESFAMRNKYTIDDNRELFAYGISNFVAAFS
CCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHC
GCSPASASVSRTAANEQFRGKTQMVSIVAATIIALIVAFLSGLLYYMPQPVLSGIVFAAL
CCCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VGIIDVDVLKGLFKVSRREATVWIVAALGTLLVGVIFGVLLGIFLSFINVVSRSMKSPIA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ILGVIEGRHGYFDLKRKPEAKPIPNVVIYRYSASLFFGNFNKFADGLKEAVQDDTKLVIF
EEEEEECCCCEECCCCCCCCCCCCCEEEEEEEHHEEECCHHHHHHHHHHHHCCCCEEEEE
EASAIINIDTTATESMKDLLKWLDDKGIEYYFADLIDHLKTSFRKHDLGYIIDNGYTKKT
EEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
VEDALEAYYAEHK
HHHHHHHHHHCCC
>Mature Secondary Structure
MFKHILLSLNGYKISYLRNDVISGVGVAALTIPVAMGYAQVAGLPPIYGLYASFLPVIAY
CCCEEEEEECCEEEEHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VIFASSPQLVFGIDATASAITGSIILGTAGLAAGSKEAIALAPILAFFCAIFLVLFSVLK
HHHCCCCCEEEEECCHHHHHHCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGRFAKYISAPVLSGFISGLSVSIIMGQIPKIMGLKESGDSFFSSLGIIFGQFFQSNWIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
FAMGVVTVIIVITCKKVIPKIPMSLVVLVLGTMAAYFFKLDQYNVDIVGKIPVGFPSLAL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
PDFGASSWALAIGGGLVCAIATFAGSLLPSESFAMRNKYTIDDNRELFAYGISNFVAAFS
CCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCHHHHHHHHHHHHHHC
GCSPASASVSRTAANEQFRGKTQMVSIVAATIIALIVAFLSGLLYYMPQPVLSGIVFAAL
CCCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VGIIDVDVLKGLFKVSRREATVWIVAALGTLLVGVIFGVLLGIFLSFINVVSRSMKSPIA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ILGVIEGRHGYFDLKRKPEAKPIPNVVIYRYSASLFFGNFNKFADGLKEAVQDDTKLVIF
EEEEEECCCCEECCCCCCCCCCCCCEEEEEEEHHEEECCHHHHHHHHHHHHCCCCEEEEE
EASAIINIDTTATESMKDLLKWLDDKGIEYYFADLIDHLKTSFRKHDLGYIIDNGYTKKT
EEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
VEDALEAYYAEHK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; SO42- [Periplasm] [C]

Specific reaction: Proton [Periplasm] + SO42- [Periplasm] = Proton [Cytoplasm] + SO42- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]