Click here to switch to the map view.

The map label for this gene is codB [H]

Identifier: 226223083

GI number: 226223083

Start: 492005

End: 493288

Strand: Direct

Name: codB [H]

Synonym: Lm4b_00480

Alternate gene names: 226223083

Gene position: 492005-493288 (Clockwise)

Preceding gene: 226223082

Following gene: 226223084

Centisome position: 16.89

GC content: 39.56

Gene sequence:

>1284_bases
ATGACAGAGAAGAAATCAGTGGAGCAAACGCAATCCTGGCAGAGTTTAGCATTTGTATGGACCGGAGCAATGATTTGTGT
ACCGAGTCTACTAGTTGGAGGAACTTTAATTTCAGGTATGCCTCTTTGGGAAGCAATTTTAATAGCACTTTTAGGATACG
GCGTTATTGTCTTATTTATGATATATCAAGGAATGCAAAGTAGCGATTTAGGTATTCCAGCAGTTAGTGTAGCTTCACAG
GTTTTTGGAGAACAAGGGTCGAAAAAAATCATATCTATTCTTTTAGCGATTGCTTGTCTAGGATGGTTTGGTATTCAAGC
CAATGTATGTGGTGCAGCATTCTCACAGTTTCTAGGTATTTATGGAATTAATCTTCCAGTACCAGTTTCTTCCTTAATTT
GGGGAGTTATCATGTTACTTTCAGCTATTTATGGTATTCGTATTTTGAGTATTTTAAATTATATCGCAGTACCAGTTTTA
CTATTTGTATGTATTTACGGACTTGTTGTTTCTTTAAACGATGGCGGACTAGCTATTGTTGAAAATTATAAACCAGCAGG
AAGCATGTCGTTTATGACAGGACTAGCAATTACAATTGGCTCATTTGCATTAGGTGCAGTAATAGCAGGAGACTATTCTC
AATATACAAAATCGCGTAAAGATGTTGTAAAAGCAGCTATTGTCGGGATTTTGCCATCAGGTGTGCTTATGATTACGGTT
GGAGCGGTACTTGCACTTACGGCGGGTACAGCAGATATTTCTGTCGTATTTACAAACTTAGGATTACCAGTTCTTGGTAT
TATCGGTTTGATACTTGCTGCATGGACAACAAACGCAGTAAATGCCTTTTCGGGAGGTATTGCGATTATCAATGTATTTA
ACATCTCTGAAAAATATCACAAAGTAGCAGTGGCAGTTGCAGGCGGACTTGGAACAATACTCGCGGTTATCGGAATTTTA
AACCACTTTGTTCCAATTATGTCCGTACTTTCAGCAATGGTACCACCCGTTGCAGGAGTAATGATTGCTTCTTACTGGAT
TGTCCAAAAAGGTGACAAATCCAAATGGGCACCAGTATCAGGTGTCAACTGGCTTGGTGTAAGTTCTTGGCTAGTTGGTG
CAGTCATTGCGGGGATTCCAGTCATTTTGACATTTTTCCCGGAGCTACCACAATTACCAAATCAACCACTTATCGGTATT
ATCTTGTCGCTGGCTGTTTACTTGATCGGAGCGAAAGTGCTGAGCGGGAAAACCAACAATGTAGAAAACTTGGAGGGAAA
ATAA

Upstream 100 bases:

>100_bases
TTGCGCTATTTTTTTTCAATTCTGATAAATACAAATGATAGCTAGTTCTTATATAATTAGTCATTAATATAACAAATTCA
AGAAAAAGGGGCGGAACATA

Downstream 100 bases:

>100_bases
ATGCGTTATTTAGATGAACAAGCTATTGAAAATATCGCGATTGGTGCTGCGTTTCTTGGCACTGGTGGCGGAGGAGATCC
ATATATAGGCAAGATGATGG

Product: permease

Products: Proton [Cytoplasm]; cytosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTEKKSVEQTQSWQSLAFVWTGAMICVPSLLVGGTLISGMPLWEAILIALLGYGVIVLFMIYQGMQSSDLGIPAVSVASQ
VFGEQGSKKIISILLAIACLGWFGIQANVCGAAFSQFLGIYGINLPVPVSSLIWGVIMLLSAIYGIRILSILNYIAVPVL
LFVCIYGLVVSLNDGGLAIVENYKPAGSMSFMTGLAITIGSFALGAVIAGDYSQYTKSRKDVVKAAIVGILPSGVLMITV
GAVLALTAGTADISVVFTNLGLPVLGIIGLILAAWTTNAVNAFSGGIAIINVFNISEKYHKVAVAVAGGLGTILAVIGIL
NHFVPIMSVLSAMVPPVAGVMIASYWIVQKGDKSKWAPVSGVNWLGVSSWLVGAVIAGIPVILTFFPELPQLPNQPLIGI
ILSLAVYLIGAKVLSGKTNNVENLEGK

Sequences:

>Translated_427_residues
MTEKKSVEQTQSWQSLAFVWTGAMICVPSLLVGGTLISGMPLWEAILIALLGYGVIVLFMIYQGMQSSDLGIPAVSVASQ
VFGEQGSKKIISILLAIACLGWFGIQANVCGAAFSQFLGIYGINLPVPVSSLIWGVIMLLSAIYGIRILSILNYIAVPVL
LFVCIYGLVVSLNDGGLAIVENYKPAGSMSFMTGLAITIGSFALGAVIAGDYSQYTKSRKDVVKAAIVGILPSGVLMITV
GAVLALTAGTADISVVFTNLGLPVLGIIGLILAAWTTNAVNAFSGGIAIINVFNISEKYHKVAVAVAGGLGTILAVIGIL
NHFVPIMSVLSAMVPPVAGVMIASYWIVQKGDKSKWAPVSGVNWLGVSSWLVGAVIAGIPVILTFFPELPQLPNQPLIGI
ILSLAVYLIGAKVLSGKTNNVENLEGK
>Mature_426_residues
TEKKSVEQTQSWQSLAFVWTGAMICVPSLLVGGTLISGMPLWEAILIALLGYGVIVLFMIYQGMQSSDLGIPAVSVASQV
FGEQGSKKIISILLAIACLGWFGIQANVCGAAFSQFLGIYGINLPVPVSSLIWGVIMLLSAIYGIRILSILNYIAVPVLL
FVCIYGLVVSLNDGGLAIVENYKPAGSMSFMTGLAITIGSFALGAVIAGDYSQYTKSRKDVVKAAIVGILPSGVLMITVG
AVLALTAGTADISVVFTNLGLPVLGIIGLILAAWTTNAVNAFSGGIAIINVFNISEKYHKVAVAVAGGLGTILAVIGILN
HFVPIMSVLSAMVPPVAGVMIASYWIVQKGDKSKWAPVSGVNWLGVSSWLVGAVIAGIPVILTFFPELPQLPNQPLIGII
LSLAVYLIGAKVLSGKTNNVENLEGK

Specific function: Required for cytosine transport into the cell [H]

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI1786530, Length=377, Percent_Identity=29.7082228116711, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 44538; Mature: 44407

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKKSVEQTQSWQSLAFVWTGAMICVPSLLVGGTLISGMPLWEAILIALLGYGVIVLFM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IYQGMQSSDLGIPAVSVASQVFGEQGSKKIISILLAIACLGWFGIQANVCGAAFSQFLGI
HHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YGINLPVPVSSLIWGVIMLLSAIYGIRILSILNYIAVPVLLFVCIYGLVVSLNDGGLAIV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEE
ENYKPAGSMSFMTGLAITIGSFALGAVIAGDYSQYTKSRKDVVKAAIVGILPSGVLMITV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GAVLALTAGTADISVVFTNLGLPVLGIIGLILAAWTTNAVNAFSGGIAIINVFNISEKYH
HHHHHHHCCCCHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHH
KVAVAVAGGLGTILAVIGILNHFVPIMSVLSAMVPPVAGVMIASYWIVQKGDKSKWAPVS
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCC
GVNWLGVSSWLVGAVIAGIPVILTFFPELPQLPNQPLIGIILSLAVYLIGAKVLSGKTNN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
VENLEGK
CCCCCCC
>Mature Secondary Structure 
TEKKSVEQTQSWQSLAFVWTGAMICVPSLLVGGTLISGMPLWEAILIALLGYGVIVLFM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
IYQGMQSSDLGIPAVSVASQVFGEQGSKKIISILLAIACLGWFGIQANVCGAAFSQFLGI
HHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
YGINLPVPVSSLIWGVIMLLSAIYGIRILSILNYIAVPVLLFVCIYGLVVSLNDGGLAIV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEE
ENYKPAGSMSFMTGLAITIGSFALGAVIAGDYSQYTKSRKDVVKAAIVGILPSGVLMITV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
GAVLALTAGTADISVVFTNLGLPVLGIIGLILAAWTTNAVNAFSGGIAIINVFNISEKYH
HHHHHHHCCCCHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHH
KVAVAVAGGLGTILAVIGILNHFVPIMSVLSAMVPPVAGVMIASYWIVQKGDKSKWAPVS
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCC
GVNWLGVSSWLVGAVIAGIPVILTFFPELPQLPNQPLIGIILSLAVYLIGAKVLSGKTNN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
VENLEGK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; cytosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + cytosine [Periplasm] = Proton [Cytoplasm] + cytosine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]