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The map label for this gene is lacA [C]

Identifier: 226223057

GI number: 226223057

Start: 457029

End: 457598

Strand: Direct

Name: lacA [C]

Synonym: Lm4b_00451

Alternate gene names: 226223057

Gene position: 457029-457598 (Clockwise)

Preceding gene: 226223055

Following gene: 226223058

Centisome position: 15.69

GC content: 40.0

Gene sequence:

>570_bases
ATGATAGAACAAGATTTACAGAAAAGACTAGTCGACCAAGAAATTCTCCAAGGGTCGGATTTGTTAGATGAAATTCACGA
AGTAAAACAAAACAATGAACAACTTATCATCGAACTAAACACCAAGTACCACTCAAAAAAAGAAGTGACCAAATACCTCA
GTGACATCACTGGAAAACCAGTCGATCCATCAGTCGATGTCTCACTTCCTTTTTATAGCGACTTCGGGAAACATATTACT
TTTGGGAAAAATATCTTTATCAATTTAAATGTAACTTTCGTGGATTTGGGTGGGATTACAATTGAAGATAATGTTTTAAT
TGGCCCAGGAGCAAGACTTGTGACCGTCAACCATTTAGTTAGCCCGAAAAAAAGACGTGGCTTAAGAGTAGCCCCAATTT
GTGTGAAGAAAAACGCCTGGATTGGCGCAAACGCAACGATATTATCAGGCGTAACAATCGGCGAAAATGCTATCGTTGCT
GCGGATGCTACCGTCACAAGAGATGTCCCAGCAAACGTTATCGTCGCCGGAAGCCCAGCCAAACAAATTCGTAAAATTAT
CGAGGAATAA

Upstream 100 bases:

>100_bases
ATAACTAAAATGAATAGTTGGATATGCTTTATAAGTATTATTCAATTATAACTTCTCCCGCTATAATTAAATAGTAAAAG
GTTTTCGAGGGGGAAAGCGC

Downstream 100 bases:

>100_bases
GGAGAGATAGCAAATGACAATCAAAAATAAAGTAATTATTATAACCGGAGCATCATCCGGAATTGGTGAAGCAACAGCCT
TACTTTTAGCTGAAAAAGGG

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MIEQDLQKRLVDQEILQGSDLLDEIHEVKQNNEQLIIELNTKYHSKKEVTKYLSDITGKPVDPSVDVSLPFYSDFGKHIT
FGKNIFINLNVTFVDLGGITIEDNVLIGPGARLVTVNHLVSPKKRRGLRVAPICVKKNAWIGANATILSGVTIGENAIVA
ADATVTRDVPANVIVAGSPAKQIRKIIEE

Sequences:

>Translated_189_residues
MIEQDLQKRLVDQEILQGSDLLDEIHEVKQNNEQLIIELNTKYHSKKEVTKYLSDITGKPVDPSVDVSLPFYSDFGKHIT
FGKNIFINLNVTFVDLGGITIEDNVLIGPGARLVTVNHLVSPKKRRGLRVAPICVKKNAWIGANATILSGVTIGENAIVA
ADATVTRDVPANVIVAGSPAKQIRKIIEE
>Mature_189_residues
MIEQDLQKRLVDQEILQGSDLLDEIHEVKQNNEQLIIELNTKYHSKKEVTKYLSDITGKPVDPSVDVSLPFYSDFGKHIT
FGKNIFINLNVTFVDLGGITIEDNVLIGPGARLVTVNHLVSPKKRRGLRVAPICVKKNAWIGANATILSGVTIGENAIVA
ADATVTRDVPANVIVAGSPAKQIRKIIEE

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=170, Percent_Identity=34.7058823529412, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786664, Length=185, Percent_Identity=31.8918918918919, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788367, Length=128, Percent_Identity=28.90625, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=122, Percent_Identity=41.8032786885246, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20733; Mature: 20733

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQDLQKRLVDQEILQGSDLLDEIHEVKQNNEQLIIELNTKYHSKKEVTKYLSDITGKP
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCC
VDPSVDVSLPFYSDFGKHITFGKNIFINLNVTFVDLGGITIEDNVLIGPGARLVTVNHLV
CCCCCEEEECHHHCCCCEEEECCEEEEEEEEEEEEECCEEEECCEEECCCCEEEEEECCC
SPKKRRGLRVAPICVKKNAWIGANATILSGVTIGENAIVAADATVTRDVPANVIVAGSPA
CHHHHCCCEEEEEEEECCCCCCCCCEEEECEEECCCEEEEECCEEECCCCCCEEEECCCH
KQIRKIIEE
HHHHHHHCC
>Mature Secondary Structure
MIEQDLQKRLVDQEILQGSDLLDEIHEVKQNNEQLIIELNTKYHSKKEVTKYLSDITGKP
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCC
VDPSVDVSLPFYSDFGKHITFGKNIFINLNVTFVDLGGITIEDNVLIGPGARLVTVNHLV
CCCCCEEEECHHHCCCCEEEECCEEEEEEEEEEEEECCEEEECCEEECCCCEEEEEECCC
SPKKRRGLRVAPICVKKNAWIGANATILSGVTIGENAIVAADATVTRDVPANVIVAGSPA
CHHHHCCCEEEEEEEECCCCCCCCCEEEECEEECCCEEEEECCEEECCCCCCEEEECCCH
KQIRKIIEE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA