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The map label for this gene is pit [H]

Identifier: 226223035

GI number: 226223035

Start: 434044

End: 435051

Strand: Direct

Name: pit [H]

Synonym: Lm4b_00429

Alternate gene names: 226223035

Gene position: 434044-435051 (Clockwise)

Preceding gene: 226223034

Following gene: 226223036

Centisome position: 14.9

GC content: 40.87

Gene sequence:

>1008_bases
ATGGATACGGTTATTTTAATTACGGTTATTATCGTTATAATGGGCTTAGCATTTGATTTTATAAATGGATTTCATGATAT
TGCAAATGCTGTGGCCACAAGTATTTCAACTAGAGCTTTAAAACCGCGAGTGGCAATTGGAATTGCGGCAGTCATGAATT
TTCTGGGAGCCATTTCGTTTACCGGAGTAGCTGAATCGCTCACCAAAAGTATTGTAGATCCATTTTCGCTTAATAATGGA
GAGTTTGTCGTTTTATGCGGCTTAATTGCGGCAGTGATTTGGAATTTGATGACTTGGCTTGTTGGCATGCCTAGTAGTTC
CTCACATGCGCTTATCGGTGCGATAGCTGGGGCCTCCATCGCATCAGCCAGTAGTTTTAGCGTGCTTAATTGGTCAGGAT
TTACAACTATTATTATTGCACTCATTATCTCGCCGATTATCGGTTTTACAGTAGGTTATTTGATTTATTCACTCTTTAAG
CATCTTTTTCACGACAAAAAGCTTGGGAAGATGAATCGACGCTTTCGTTTTATCCAAATTGGAACAGCTGCCGCCCAAGC
ATATTCACACGGCACAAATGATGCCCAAAAAACGATGGGAATTATCACGCTTGCTTTAGTTGCGAGTGGGCTTTTGAAAG
AGTCAGCTGGTATTCCTTTTTGGGTGCAAGTAAGTTGTGCGGCATCGATGGCGATTGGATCGTCTGTTGGTGGTTATCGA
ATCATCAAAACAGTAGGAACTAAAATCATGAAAATCACCCCAGTTACAGGTGTTGCGTCCGATTTAAGCTCACTTTCTGT
AATCATGACAGCTACTTTGATTCATTTGCCAGTCAGCACTACGCAAGTTATTGACAGCTCGATTATGGGTGTCGGAACAG
CTAACCATAAAAAAGAAGTCAATTGGCGTACGGGAAAAAATATGGTTGTAACTTGGTTTATCACGTTACCACTAGCTGGA
CTTTTGGCAGCAGTGGTATACTGGATATCAGCAGCTATTTTTTTATAA

Upstream 100 bases:

>100_bases
ACGAGAATCTGCTACCTAATACAGGAGCAGATTCTTTTTATATTTCTATAAAAGGGGTATAGACAATAAAAAAGTGAATA
TCGACAAGGGGAACTGCTGA

Downstream 100 bases:

>100_bases
TAAGGAGGATTTTAAATGAAAATAGAACATGTTGCATTATGGACAACTAATTTAGAACAAATGAAGCAATTTTACGTCAC
TTATTTTGGTGCAACGGCCA

Product: phosphate transport protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MDTVILITVIIVIMGLAFDFINGFHDIANAVATSISTRALKPRVAIGIAAVMNFLGAISFTGVAESLTKSIVDPFSLNNG
EFVVLCGLIAAVIWNLMTWLVGMPSSSSHALIGAIAGASIASASSFSVLNWSGFTTIIIALIISPIIGFTVGYLIYSLFK
HLFHDKKLGKMNRRFRFIQIGTAAAQAYSHGTNDAQKTMGIITLALVASGLLKESAGIPFWVQVSCAASMAIGSSVGGYR
IIKTVGTKIMKITPVTGVASDLSSLSVIMTATLIHLPVSTTQVIDSSIMGVGTANHKKEVNWRTGKNMVVTWFITLPLAG
LLAAVVYWISAAIFL

Sequences:

>Translated_335_residues
MDTVILITVIIVIMGLAFDFINGFHDIANAVATSISTRALKPRVAIGIAAVMNFLGAISFTGVAESLTKSIVDPFSLNNG
EFVVLCGLIAAVIWNLMTWLVGMPSSSSHALIGAIAGASIASASSFSVLNWSGFTTIIIALIISPIIGFTVGYLIYSLFK
HLFHDKKLGKMNRRFRFIQIGTAAAQAYSHGTNDAQKTMGIITLALVASGLLKESAGIPFWVQVSCAASMAIGSSVGGYR
IIKTVGTKIMKITPVTGVASDLSSLSVIMTATLIHLPVSTTQVIDSSIMGVGTANHKKEVNWRTGKNMVVTWFITLPLAG
LLAAVVYWISAAIFL
>Mature_335_residues
MDTVILITVIIVIMGLAFDFINGFHDIANAVATSISTRALKPRVAIGIAAVMNFLGAISFTGVAESLTKSIVDPFSLNNG
EFVVLCGLIAAVIWNLMTWLVGMPSSSSHALIGAIAGASIASASSFSVLNWSGFTTIIIALIISPIIGFTVGYLIYSLFK
HLFHDKKLGKMNRRFRFIQIGTAAAQAYSHGTNDAQKTMGIITLALVASGLLKESAGIPFWVQVSCAASMAIGSSVGGYR
IIKTVGTKIMKITPVTGVASDLSSLSVIMTATLIHLPVSTTQVIDSSIMGVGTANHKKEVNWRTGKNMVVTWFITLPLAG
LLAAVVYWISAAIFL

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=166, Percent_Identity=31.9277108433735, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=35.7142857142857, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=39.1705069124424, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=40.0921658986175, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17539280, Length=157, Percent_Identity=31.2101910828025, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25146401, Length=175, Percent_Identity=28, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566716, Length=180, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71981576, Length=157, Percent_Identity=25.4777070063694, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=183, Percent_Identity=27.3224043715847, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=170, Percent_Identity=30.5882352941176, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356511, Length=186, Percent_Identity=26.8817204301075, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35414; Mature: 35414

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVILITVIIVIMGLAFDFINGFHDIANAVATSISTRALKPRVAIGIAAVMNFLGAISF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAESLTKSIVDPFSLNNGEFVVLCGLIAAVIWNLMTWLVGMPSSSSHALIGAIAGASI
HHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHH
ASASSFSVLNWSGFTTIIIALIISPIIGFTVGYLIYSLFKHLFHDKKLGKMNRRFRFIQI
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEE
GTAAAQAYSHGTNDAQKTMGIITLALVASGLLKESAGIPFWVQVSCAASMAIGSSVGGYR
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCCHH
IIKTVGTKIMKITPVTGVASDLSSLSVIMTATLIHLPVSTTQVIDSSIMGVGTANHKKEV
HHHHHCCHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
NWRTGKNMVVTWFITLPLAGLLAAVVYWISAAIFL
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTVILITVIIVIMGLAFDFINGFHDIANAVATSISTRALKPRVAIGIAAVMNFLGAISF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAESLTKSIVDPFSLNNGEFVVLCGLIAAVIWNLMTWLVGMPSSSSHALIGAIAGASI
HHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHH
ASASSFSVLNWSGFTTIIIALIISPIIGFTVGYLIYSLFKHLFHDKKLGKMNRRFRFIQI
CCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEE
GTAAAQAYSHGTNDAQKTMGIITLALVASGLLKESAGIPFWVQVSCAASMAIGSSVGGYR
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCCCCHH
IIKTVGTKIMKITPVTGVASDLSSLSVIMTATLIHLPVSTTQVIDSSIMGVGTANHKKEV
HHHHHCCHHEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCC
NWRTGKNMVVTWFITLPLAGLLAAVVYWISAAIFL
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]