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The map label for this gene is fruA [H]

Identifier: 226222985

GI number: 226222985

Start: 387348

End: 388742

Strand: Direct

Name: fruA [H]

Synonym: Lm4b_00377

Alternate gene names: 226222985

Gene position: 387348-388742 (Clockwise)

Preceding gene: 226222984

Following gene: 226222986

Centisome position: 13.3

GC content: 41.86

Gene sequence:

>1395_bases
ATGAAAATAGTTGGTGTTACTGCTTGTCCCACTGGTATTGCGCATACGTATATGTCTGCCGAGAAATTAACTATAACGGC
AGAAGCGCTTGGTTACGAAGTGAAAATCGAGACACAAGGAGCTAAGGTTGAAAATGTTTTAACGAAGGAAGACATCGCGA
CTGCTGATTATGTCATTTTAGCAGTCGATAAAGAAATAGATACATCAAGGTTCGCTGGAAAGAAAATAAAAAAAGTATCC
ACTTCCAGAGCGATTAAAGAGGCTGATGTGGTTATTGAGGAAACTATTTCTGGTAAAGGGTTAATCAGTTTGGAGGCGAA
AAGTTCGGATATAAGTTCGGAACAACCGTCAAAAGCAAGTTTATACAATCACTTTATGAATGGTGTTAACTATATGTTGC
CATTTGTCATAGCTGGCGGGATTTTAATTGCTATTAGTTTTGCTTTTGGAATTGATGCCTCAAACCCTGACTCTGATTCG
TATAACGCACTTGCAGCTGCATTTTCAAAAATTGGTGGGGATACTGCATTTGGTTTAATGGTTCCAGCCCTTGCAGCAGG
GATAGCGGTTTCTGTGGCTGGTCGAGCAGGATTTGCGCCCGGGCTTGTGGCAGGGACACTGGCGACTGTTGGTGGATCAG
GATTTCTAGGCGGTATGATTGGTGGTATTTTGGCTGGTTACGTGGCACATTTCTTTGCTAATAAAGTCAATGTCACTAAA
TCACTTGCTTCGATTTATCAATTGATTGTTGTACCGCTTTTAGGGATTACGATTGTTGGTTTGGCGATGGTATTTATTAT
TGATACACCAATCGCTTGGGTTTTAAATGCACTCACAGGTTGGTTGAATGGGCTTGGTGAGACATCTGGCGTCGTATTTG
GACTACTCATTGGTGTGATGATGGCAGCAGATATGGGTGGGCCAATTAATAAATCTATTTCAACCTTTTCAATTGGGTTA
ATGTCAGCAGGTGTAACGGCGCCGATTGCGGCTTGTATGGCGGCTGGAATGGTTCCGCCACTTGGATTAGCACTCGCAAC
ACTATTATTTAAAAACAAATTTACAAAAGAAGAAAAAACAGCGGGGAATTCTTGTTGGGTGTTAGGTGCATCTTATATTA
CTGAAGGAGCAATTCCTTTCGCAGTTGCTGATCCGCTCCGGGTTATCCCAAGTTTGATGTTAGGTTCTGCAACAGCTGCT
GCAATTTCGATGGGAGCTGGTGTAACTTCGATGGCGCCTCACGGTGGGATTTGGGTGATGTTCATTCCAAATGTAATTAA
CCATTTATTTATCTACTTACTAGCAATTGCAGCAGGCACAGTCGTGACAGCAATTTCTGTCGGACTATTAAAAACCCCAT
TAAACAAGCGAAAAAATAAAGAGGAGATGATATAA

Upstream 100 bases:

>100_bases
TCGAGGAAGTTAATGAACAAAGAATTTCGTGAAAAATTAAAAAATTCCAAAAACAAAACGGAAATTATAACAATTTTATC
GAACTAAGGAGGCGTTTCAC

Downstream 100 bases:

>100_bases
TGTTATATACAATGAAAGATCTTTTAGCAGTAGGAAAAGAGCATCAATTTGCGGTACCAGCATTTAATATCTGTAGTTTT
GATATGTTAAAGGCGATAAT

Product: PTS system, fructose-specific enzyme IIBC component

Products: NA

Alternate protein names: EIIBBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MKIVGVTACPTGIAHTYMSAEKLTITAEALGYEVKIETQGAKVENVLTKEDIATADYVILAVDKEIDTSRFAGKKIKKVS
TSRAIKEADVVIEETISGKGLISLEAKSSDISSEQPSKASLYNHFMNGVNYMLPFVIAGGILIAISFAFGIDASNPDSDS
YNALAAAFSKIGGDTAFGLMVPALAAGIAVSVAGRAGFAPGLVAGTLATVGGSGFLGGMIGGILAGYVAHFFANKVNVTK
SLASIYQLIVVPLLGITIVGLAMVFIIDTPIAWVLNALTGWLNGLGETSGVVFGLLIGVMMAADMGGPINKSISTFSIGL
MSAGVTAPIAACMAAGMVPPLGLALATLLFKNKFTKEEKTAGNSCWVLGASYITEGAIPFAVADPLRVIPSLMLGSATAA
AISMGAGVTSMAPHGGIWVMFIPNVINHLFIYLLAIAAGTVVTAISVGLLKTPLNKRKNKEEMI

Sequences:

>Translated_464_residues
MKIVGVTACPTGIAHTYMSAEKLTITAEALGYEVKIETQGAKVENVLTKEDIATADYVILAVDKEIDTSRFAGKKIKKVS
TSRAIKEADVVIEETISGKGLISLEAKSSDISSEQPSKASLYNHFMNGVNYMLPFVIAGGILIAISFAFGIDASNPDSDS
YNALAAAFSKIGGDTAFGLMVPALAAGIAVSVAGRAGFAPGLVAGTLATVGGSGFLGGMIGGILAGYVAHFFANKVNVTK
SLASIYQLIVVPLLGITIVGLAMVFIIDTPIAWVLNALTGWLNGLGETSGVVFGLLIGVMMAADMGGPINKSISTFSIGL
MSAGVTAPIAACMAAGMVPPLGLALATLLFKNKFTKEEKTAGNSCWVLGASYITEGAIPFAVADPLRVIPSLMLGSATAA
AISMGAGVTSMAPHGGIWVMFIPNVINHLFIYLLAIAAGTVVTAISVGLLKTPLNKRKNKEEMI
>Mature_464_residues
MKIVGVTACPTGIAHTYMSAEKLTITAEALGYEVKIETQGAKVENVLTKEDIATADYVILAVDKEIDTSRFAGKKIKKVS
TSRAIKEADVVIEETISGKGLISLEAKSSDISSEQPSKASLYNHFMNGVNYMLPFVIAGGILIAISFAFGIDASNPDSDS
YNALAAAFSKIGGDTAFGLMVPALAAGIAVSVAGRAGFAPGLVAGTLATVGGSGFLGGMIGGILAGYVAHFFANKVNVTK
SLASIYQLIVVPLLGITIVGLAMVFIIDTPIAWVLNALTGWLNGLGETSGVVFGLLIGVMMAADMGGPINKSISTFSIGL
MSAGVTAPIAACMAAGMVPPLGLALATLLFKNKFTKEEKTAGNSCWVLGASYITEGAIPFAVADPLRVIPSLMLGSATAA
AISMGAGVTSMAPHGGIWVMFIPNVINHLFIYLLAIAAGTVVTAISVGLLKTPLNKRKNKEEMI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=455, Percent_Identity=44.3956043956044, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786951, Length=428, Percent_Identity=35.7476635514019, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87082348, Length=476, Percent_Identity=32.7731092436975, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790386, Length=298, Percent_Identity=38.255033557047, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1788729, Length=397, Percent_Identity=27.9596977329975, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790387, Length=93, Percent_Identity=55.9139784946236, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788730, Length=96, Percent_Identity=44.7916666666667, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47744; Mature: 47744

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVGVTACPTGIAHTYMSAEKLTITAEALGYEVKIETQGAKVENVLTKEDIATADYVIL
CEEEEEECCCCHHHHHHCCCCCEEEEHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEE
AVDKEIDTSRFAGKKIKKVSTSRAIKEADVVIEETISGKGLISLEAKSSDISSEQPSKAS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHH
LYNHFMNGVNYMLPFVIAGGILIAISFAFGIDASNPDSDSYNALAAAFSKIGGDTAFGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
VPALAAGIAVSVAGRAGFAPGLVAGTLATVGGSGFLGGMIGGILAGYVAHFFANKVNVTK
HHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLASIYQLIVVPLLGITIVGLAMVFIIDTPIAWVLNALTGWLNGLGETSGVVFGLLIGVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MAADMGGPINKSISTFSIGLMSAGVTAPIAACMAAGMVPPLGLALATLLFKNKFTKEEKT
HHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHC
AGNSCWVLGASYITEGAIPFAVADPLRVIPSLMLGSATAAAISMGAGVTSMAPHGGIWVM
CCCCEEEEEHHHHHCCCCCHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEE
FIPNVINHLFIYLLAIAAGTVVTAISVGLLKTPLNKRKNKEEMI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCC
>Mature Secondary Structure
MKIVGVTACPTGIAHTYMSAEKLTITAEALGYEVKIETQGAKVENVLTKEDIATADYVIL
CEEEEEECCCCHHHHHHCCCCCEEEEHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEE
AVDKEIDTSRFAGKKIKKVSTSRAIKEADVVIEETISGKGLISLEAKSSDISSEQPSKAS
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHH
LYNHFMNGVNYMLPFVIAGGILIAISFAFGIDASNPDSDSYNALAAAFSKIGGDTAFGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
VPALAAGIAVSVAGRAGFAPGLVAGTLATVGGSGFLGGMIGGILAGYVAHFFANKVNVTK
HHHHHHHHHHEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SLASIYQLIVVPLLGITIVGLAMVFIIDTPIAWVLNALTGWLNGLGETSGVVFGLLIGVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MAADMGGPINKSISTFSIGLMSAGVTAPIAACMAAGMVPPLGLALATLLFKNKFTKEEKT
HHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHC
AGNSCWVLGASYITEGAIPFAVADPLRVIPSLMLGSATAAAISMGAGVTSMAPHGGIWVM
CCCCEEEEEHHHHHCCCCCHHHCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEE
FIPNVINHLFIYLLAIAAGTVVTAISVGLLKTPLNKRKNKEEMI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254279 [H]