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The map label for this gene is bglH [H]

Identifier: 226222892

GI number: 226222892

Start: 282671

End: 284131

Strand: Direct

Name: bglH [H]

Synonym: Lm4b_00284

Alternate gene names: 226222892

Gene position: 282671-284131 (Clockwise)

Preceding gene: 226222891

Following gene: 226222893

Centisome position: 9.7

GC content: 40.66

Gene sequence:

>1461_bases
ATGACTGAAGTGAAAAAACAATTTCCAAAAGGATTTTTATGGGGTGGAGCAACTGCTGCGAACCAAGTTGAAGGCGCTTA
CGATGTAGATGGTAAAGGCCTTTCCACAGCAGATATGGTCAAATTCATTCCAAAAGAAGAACGCACACAAGATCATGCAT
TAGATGTATCTAAAGCAGAAATCGAAGCAATTATTGCAGGGAAAGTAGAAGGTAGATTCCCTAAACGTGATGGCGTTGAT
TTTTATCATCACTATAAAGAAGACATCGCATTATTTGCCGAAATGGGCTTTAAAACATTCCGACTATCACTAAACTGGGC
GCGTATTTTTCCGAACGGTGATGACAAAGAGCCGAATGAAAAAGGCCTTGAATTTTATGATAAAGTGTTTGATGAATTAT
TAAAATATGACATTGAGCCACTTGTGACACTTTCTCACTATGAAACACCACTAAATCTTACTTTAAAATATAACGGCTGG
GCTGATCGTCGCGTTATCGGATTTTTCACGAACTATGCGGAAACAGTTTTCAAACGTTATAAAAACAAAGTAAAATATTG
GCTAACTTTTAACGAAATCAATGTTATTTCACTTAGCGCATACACTGGTGGTGGCGTGCTTTTAGAAGACGCGAAGAATC
CACTTGAACTTTCTTATCAAGCAGGACATCACCAATTCGTAGCCAGTGCTCTTGCAACAAAATTGGCGCATGAAATTATT
CCTGGATCACAAGTTGGGTGTATGTTAGCGCGCATGGCGACTTATCCTGCGACTAACAACCCAGATGATATCCTAAAAGC
ACAATACGAAAACCAACAAAATCTATTTTTCACAGACGTTCATGCTCGCGGTGAATATCCAAGCTTCATGAATCGTTTCT
TCCAAGAGAACGACATTCACATCGTAAAAGAAGTTGGCGACGACGAAATCTTGAAAGCACATACAGTTGATTTCATTTCA
TTCAGCTATTACATGTCCCTATCCGCAACAGCTAGCCCAGAAGGCGACCGCTCTGCCGGGAACTTAATGGGTGGCGTGAA
AAATGAATACTTGGAGTCGTCCGATTGGGGTTGGCAAATTGACCCTAAAGGCTTACGCTGGACACTAAATGATCTTTATA
GCCGCTACGAATTACCACTTTTCATCGTGGAAAATGGCTTAGGTGCTTATGATACAGTGGAAGAAGACGGCAAAATCCAT
GACGATTACCGAATTGATTACTTGCGCAAACACATCGAACAAATGAAAGAAGCAATTGCAGACGGCGTAGATTTAATTGG
TTACACAAGCTGGGGACCTATCGACCTAGTAAGTGCCTCCACAAGCGAAATGTCCAAACGTTACGGCTTCATCTACGTAG
ACCAAGATGACTGGGGCAAAGGAACATTAGAACGCTCCCGCAAAGATTCATTCTTCTGGTATAAAAAAGTAATTGAGACA
AATGGTGAAGATTTAGTTTAA

Upstream 100 bases:

>100_bases
AAGCCCTTTTACTATTAAAGAAAATTTCTGCTTGACCTGTAATGCATTACACAGAGTAAACTAAAAAAGTAGAAGAAATT
AGCCGGAAGGGGCGAACGAA

Downstream 100 bases:

>100_bases
TAGAAAGTTAGCAGTCCCGCTTTGGCGGGGCTGTTTTTTATGAATGTAAAAGCGAAGTATTTATCTTTTAGCATGAATTA
AATTAAAAAATATTAATAAT

Product: phospho-beta-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTEVKKQFPKGFLWGGATAANQVEGAYDVDGKGLSTADMVKFIPKEERTQDHALDVSKAEIEAIIAGKVEGRFPKRDGVD
FYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNEKGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGW
ADRRVIGFFTNYAETVFKRYKNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLELSYQAGHHQFVASALATKLAHEII
PGSQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIHIVKEVGDDEILKAHTVDFIS
FSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQIDPKGLRWTLNDLYSRYELPLFIVENGLGAYDTVEEDGKIH
DDYRIDYLRKHIEQMKEAIADGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
NGEDLV

Sequences:

>Translated_486_residues
MTEVKKQFPKGFLWGGATAANQVEGAYDVDGKGLSTADMVKFIPKEERTQDHALDVSKAEIEAIIAGKVEGRFPKRDGVD
FYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNEKGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGW
ADRRVIGFFTNYAETVFKRYKNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLELSYQAGHHQFVASALATKLAHEII
PGSQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIHIVKEVGDDEILKAHTVDFIS
FSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQIDPKGLRWTLNDLYSRYELPLFIVENGLGAYDTVEEDGKIH
DDYRIDYLRKHIEQMKEAIADGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
NGEDLV
>Mature_485_residues
TEVKKQFPKGFLWGGATAANQVEGAYDVDGKGLSTADMVKFIPKEERTQDHALDVSKAEIEAIIAGKVEGRFPKRDGVDF
YHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNEKGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGWA
DRRVIGFFTNYAETVFKRYKNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLELSYQAGHHQFVASALATKLAHEIIP
GSQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIHIVKEVGDDEILKAHTVDFISF
SYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQIDPKGLRWTLNDLYSRYELPLFIVENGLGAYDTVEEDGKIHD
DYRIDYLRKHIEQMKEAIADGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIETN
GEDLV

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=498, Percent_Identity=29.3172690763052, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI110681710, Length=502, Percent_Identity=30.2788844621514, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI13273313, Length=495, Percent_Identity=26.4646464646465, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI28376633, Length=486, Percent_Identity=28.1893004115226, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI24497614, Length=486, Percent_Identity=29.6296296296296, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1789070, Length=479, Percent_Identity=54.9060542797495, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI2367270, Length=486, Percent_Identity=54.7325102880658, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI2367174, Length=491, Percent_Identity=52.7494908350306, Blast_Score=515, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17552856, Length=497, Percent_Identity=29.1750503018109, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17539390, Length=455, Percent_Identity=28.7912087912088, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21356577, Length=481, Percent_Identity=25.987525987526, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55532; Mature: 55401

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVKKQFPKGFLWGGATAANQVEGAYDVDGKGLSTADMVKFIPKEERTQDHALDVSKAE
CCCHHHHCCCCEEECCCCHHHCCCCCEECCCCCCCHHHHHHHCCCCCCCHHHHCCCCHHH
IEAIIAGKVEGRFPKRDGVDFYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNE
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCH
KGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGWADRRVIGFFTNYAETVFKRY
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLELSYQAGHHQFVASALATKLAHEII
HCCEEEEEEECCEEEEEEEEECCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHC
PGSQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIH
CCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCE
IVKEVGDDEILKAHTVDFISFSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQI
EEEECCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHCCCCCCCEEE
DPKGLRWTLNDLYSRYELPLFIVENGLGAYDTVEEDGKIHDDYRIDYLRKHIEQMKEAIA
CCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
CCCEEEEECCCCCEEEECCCHHHHHHHCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHC
NGEDLV
CCCCCC
>Mature Secondary Structure 
TEVKKQFPKGFLWGGATAANQVEGAYDVDGKGLSTADMVKFIPKEERTQDHALDVSKAE
CCHHHHCCCCEEECCCCHHHCCCCCEECCCCCCCHHHHHHHCCCCCCCHHHHCCCCHHH
IEAIIAGKVEGRFPKRDGVDFYHHYKEDIALFAEMGFKTFRLSLNWARIFPNGDDKEPNE
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCH
KGLEFYDKVFDELLKYDIEPLVTLSHYETPLNLTLKYNGWADRRVIGFFTNYAETVFKRY
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
KNKVKYWLTFNEINVISLSAYTGGGVLLEDAKNPLELSYQAGHHQFVASALATKLAHEII
HCCEEEEEEECCEEEEEEEEECCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHC
PGSQVGCMLARMATYPATNNPDDILKAQYENQQNLFFTDVHARGEYPSFMNRFFQENDIH
CCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCE
IVKEVGDDEILKAHTVDFISFSYYMSLSATASPEGDRSAGNLMGGVKNEYLESSDWGWQI
EEEECCCCHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHCCCCCCCEEE
DPKGLRWTLNDLYSRYELPLFIVENGLGAYDTVEEDGKIHDDYRIDYLRKHIEQMKEAIA
CCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
DGVDLIGYTSWGPIDLVSASTSEMSKRYGFIYVDQDDWGKGTLERSRKDSFFWYKKVIET
CCCEEEEECCCCCEEEECCCHHHHHHHCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHC
NGEDLV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377 [H]