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The map label for this gene is gtfA [H]

Identifier: 226222887

GI number: 226222887

Start: 272655

End: 274097

Strand: Direct

Name: gtfA [H]

Synonym: Lm4b_00279

Alternate gene names: 226222887

Gene position: 272655-274097 (Clockwise)

Preceding gene: 226222886

Following gene: 226222888

Centisome position: 9.36

GC content: 32.92

Gene sequence:

>1443_bases
TTGCAAATTAAAAATAAAGCTATGTTAATTACTTATTCTGATAGTTTAGGGAAAAATATGGAAGAATTATCCAAGGTGAT
GGAAACTTATTTTGAAGATGCTGTTGGCGGGATTCACTTATTGCCGTTCTTTCCATCCACTGGAGATCGAGGGTTTGCGC
CAAGTGATTACACAACAGTAGATAGCGACCTAGGTTCTTGGGAAATAATCGAGAAATTAGGCGAAAAGTATTATTTAATG
TTTGATTTTATGATTAATCACATTTCTCGCGAATCACTCTTCTTTCAAGATTTTAAAAAAGAGCATTTAAACTCAAAGTA
TAAAGATATGTTTATTCGTATCAATGATTTCTTTCCTCCGGGTAGACCAAATGAAAAAGACTTAGATTTAATTTATAAGA
GAAAAGATAAGGCGCCTTTTCAAGAAGTTGAATTTGCTGATGGGGAAACGGAATTAGTTTGGAATACTTTTGGGGAAGAA
CAAATTGATTTAGATGTGACAGCCGAAGTTACAAAAGAATTTATTCGTCAAACGATAAAAAATATGGCTGCACATGGTTG
TTCTATTTTGCGTCTTGATGCCTTTGCTTATGCAATTAAAAAATTAGATACAAATGATTTTTTTGTAGAACCGGAAATTT
GGGATTTACTGGATGAAGTGAAAGCAGAAGCGGCCAAATATGACATGGAATTATTACCAGAAATTCATGAACATTATTCC
ATCCAAATGAAAATCGCGAACCATGATTATTATATCTATGATTTTGCGTTACCCATGGTGATGCTGTACTCGTTATATAG
TGGCCGAGTGGAACGTTTAGCTAACTGGTTAGAAATGAGTCCGATGAAGCAATTTACTACTTTAGATACGCATGATGGCA
TTGGCGTTGTGGATGCACGCGATTTATTAACAGATGAGGAACTCGATTATACTTCAGCAGAATTATACAAAATAGGAGCT
AATGTTAAAAAGATATATTCCTCTGAAAAATATAATAACCTGGATATTTATCAAATTAATAGTACCTATTATAGTGCTTT
GGGTGACGATGATAAAAGTTACTTGTTAGCGAGAGTAATTCAATGTTTTGCGCCGGGGATTCCGCAAATTTATTATGTTG
GTTTACTTGCTGGCAAAAATGATATTGACCTTTTAGAGGAAACAAAAGAAGGGCGTAATATTAATCGTCATTACTACACA
ATAGATGAAATTAAAAATGAAGTAAAAAGACCAGTTGTTAAAGCGTTGTGCAATTTACTGAGGTTTAGAAATACTTCTGA
AGCGTTTGATTTGGAAGGAAGTATAGAAATTGAGACACCTAGCTCAAATGAAATCGTTATTATTCGCAAAAACAAAACAA
ATAAAATTACAGCGACATTAAAAGCAAATTTAAGTACTAAAACATTCCAAATCAGCGAAAATGAAAGAAATATTTTAATT
TAA

Upstream 100 bases:

>100_bases
GGTACTTGCTAATGTAGCGTTGCCAGAAATGGTAGAAAATGTCCTTGAAATACCTGCATATACAGGTGCCGTCTTCTCAA
GGGTTTTGGAGGTAGACTGA

Downstream 100 bases:

>100_bases
CATTTTATAAACACGAATGAGCCTCTTCGTTCGTGTTTATTTCTATTTTCTATTGACATTGGCTAATGGTTATTGTATTA
TTATATAATGCCAAAAGAAT

Product: Sucrose phosphorylase

Products: NA

Alternate protein names: Glucosyltransferase-A; GTF-A; Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MQIKNKAMLITYSDSLGKNMEELSKVMETYFEDAVGGIHLLPFFPSTGDRGFAPSDYTTVDSDLGSWEIIEKLGEKYYLM
FDFMINHISRESLFFQDFKKEHLNSKYKDMFIRINDFFPPGRPNEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEE
QIDLDVTAEVTKEFIRQTIKNMAAHGCSILRLDAFAYAIKKLDTNDFFVEPEIWDLLDEVKAEAAKYDMELLPEIHEHYS
IQMKIANHDYYIYDFALPMVMLYSLYSGRVERLANWLEMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSAELYKIGA
NVKKIYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARVIQCFAPGIPQIYYVGLLAGKNDIDLLEETKEGRNINRHYYT
IDEIKNEVKRPVVKALCNLLRFRNTSEAFDLEGSIEIETPSSNEIVIIRKNKTNKITATLKANLSTKTFQISENERNILI

Sequences:

>Translated_480_residues
MQIKNKAMLITYSDSLGKNMEELSKVMETYFEDAVGGIHLLPFFPSTGDRGFAPSDYTTVDSDLGSWEIIEKLGEKYYLM
FDFMINHISRESLFFQDFKKEHLNSKYKDMFIRINDFFPPGRPNEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEE
QIDLDVTAEVTKEFIRQTIKNMAAHGCSILRLDAFAYAIKKLDTNDFFVEPEIWDLLDEVKAEAAKYDMELLPEIHEHYS
IQMKIANHDYYIYDFALPMVMLYSLYSGRVERLANWLEMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSAELYKIGA
NVKKIYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARVIQCFAPGIPQIYYVGLLAGKNDIDLLEETKEGRNINRHYYT
IDEIKNEVKRPVVKALCNLLRFRNTSEAFDLEGSIEIETPSSNEIVIIRKNKTNKITATLKANLSTKTFQISENERNILI
>Mature_480_residues
MQIKNKAMLITYSDSLGKNMEELSKVMETYFEDAVGGIHLLPFFPSTGDRGFAPSDYTTVDSDLGSWEIIEKLGEKYYLM
FDFMINHISRESLFFQDFKKEHLNSKYKDMFIRINDFFPPGRPNEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEE
QIDLDVTAEVTKEFIRQTIKNMAAHGCSILRLDAFAYAIKKLDTNDFFVEPEIWDLLDEVKAEAAKYDMELLPEIHEHYS
IQMKIANHDYYIYDFALPMVMLYSLYSGRVERLANWLEMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSAELYKIGA
NVKKIYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARVIQCFAPGIPQIYYVGLLAGKNDIDLLEETKEGRNINRHYYT
IDEIKNEVKRPVVKALCNLLRFRNTSEAFDLEGSIEIETPSSNEIVIIRKNKTNKITATLKANLSTKTFQISENERNILI

Specific function: Intracellular catabolism of sucrose. Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=501, Percent_Identity=27.5449101796407, Blast_Score=171, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377
- InterPro:   IPR022527 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 55651; Mature: 55651

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKNKAMLITYSDSLGKNMEELSKVMETYFEDAVGGIHLLPFFPSTGDRGFAPSDYTTV
CCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
DSDLGSWEIIEKLGEKYYLMFDFMINHISRESLFFQDFKKEHLNSKYKDMFIRINDFFPP
CCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GRPNEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEEQIDLDVTAEVTKEFIRQTIK
CCCCCCCCHHHEECCCCCCHHHEECCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHH
NMAAHGCSILRLDAFAYAIKKLDTNDFFVEPEIWDLLDEVKAEAAKYDMELLPEIHEHYS
HHHHCCCCEEEHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCEE
IQMKIANHDYYIYDFALPMVMLYSLYSGRVERLANWLEMSPMKQFTTLDTHDGIGVVDAR
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEHH
DLLTDEELDYTSAELYKIGANVKKIYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARVI
HHCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHH
QCFAPGIPQIYYVGLLAGKNDIDLLEETKEGRNINRHYYTIDEIKNEVKRPVVKALCNLL
HHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RFRNTSEAFDLEGSIEIETPSSNEIVIIRKNKTNKITATLKANLSTKTFQISENERNILI
HHCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MQIKNKAMLITYSDSLGKNMEELSKVMETYFEDAVGGIHLLPFFPSTGDRGFAPSDYTTV
CCCCCCEEEEEECCHHCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCC
DSDLGSWEIIEKLGEKYYLMFDFMINHISRESLFFQDFKKEHLNSKYKDMFIRINDFFPP
CCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GRPNEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEEQIDLDVTAEVTKEFIRQTIK
CCCCCCCCHHHEECCCCCCHHHEECCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHH
NMAAHGCSILRLDAFAYAIKKLDTNDFFVEPEIWDLLDEVKAEAAKYDMELLPEIHEHYS
HHHHCCCCEEEHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCEE
IQMKIANHDYYIYDFALPMVMLYSLYSGRVERLANWLEMSPMKQFTTLDTHDGIGVVDAR
EEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEEHH
DLLTDEELDYTSAELYKIGANVKKIYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARVI
HHCCCCCCCCCHHHHHHHCCHHHHHHCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHH
QCFAPGIPQIYYVGLLAGKNDIDLLEETKEGRNINRHYYTIDEIKNEVKRPVVKALCNLL
HHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RFRNTSEAFDLEGSIEIETPSSNEIVIIRKNKTNKITATLKANLSTKTFQISENERNILI
HHCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3194228; 2967248; 12397186; 2971020 [H]