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The map label for this gene is ykfA [H]

Identifier: 226222668

GI number: 226222668

Start: 33166

End: 34041

Strand: Direct

Name: ykfA [H]

Synonym: Lm4b_00031

Alternate gene names: 226222668

Gene position: 33166-34041 (Clockwise)

Preceding gene: 226222667

Following gene: 226222674

Centisome position: 1.14

GC content: 35.73

Gene sequence:

>876_bases
ATGTTAGCAAAGGGCGATTTGATAGGGATAATTTGTTGTTCTGATGGTCGAAAAAAAGAAGACGAGAAGGATTTGAAGCG
CCTTAAACAAGTCTTAGAAAAGGAATTTGGACTTCAGGTGGTTTTTGCGAAAACTATTTTTCAAATAGATGATTCGCCTT
TTAATGGTACTCCTGAGGAACGAGCAGCCGAATTAATGAAACTATATCAAAATATGGATGTAAAAATGATTTTTGACATT
TCAGGAGGGGATGCGGCTAATCAAGTATTACCATATCTTAATTTTAACATTATAAAAAAAGCAGCCAAACCTTTTATCGG
CTATAGCGACTTAACCGTAATATTAAATGCAATTTTTGCGAAGACGAAGCAGCCTGGCTTTAATTATTTACTGCGAAATT
TAGTAGGTGAAGCTAGCGAGATACAACGAGTCCAATTCCAAAAAACATTTTTCGAGAATCAAATTGCGATAAATGGGAAA
TCCCTTAATGAGTTTGAATGGAGCCTGGGTGAGATCGTTGGTGGAAATATCCGCTGTTTTTTAAAACTAGCAGGAACAGA
ATTTATGCCTGACGTCTCGAATAAAATAATTTTGTTAGAAAGTTTAGGTGGAAAAGAGGCGAAAATTGCTTCTTATGTAG
CGCAATTAGAACAGTTAGGTGTGTTTTCGAAATGCTTAGGAATAGTTGTTGGGGAACACACTGAGGCTGAGAAGAACGGG
GAATACGACAGAATAGGAATCTTATATCAACAGATTGGATTAAAATATAAGTTGCCTGTTTTTCGAACTAAGGAAATCGG
TCATAGCAGCGAAGCAAAACCATGTTTAATTGGCACAAAAGTTAATGTTTCACGTGAAACATTAACAAATTTTTAA

Upstream 100 bases:

>100_bases
AATCACATTAGTAATCTAAAATTCATTAATAAACAGCTTGGGCGTAAATCTGCTCAAGCTGTTTTTATGTTATACTGAGG
GAAAAATAGGCGGTGAGCGG

Downstream 100 bases:

>100_bases
TAATTTTATTGCGAAATATTCATAATTAACCTTTAATATAGAACTTGTAAGCAAGGAATACAATTAGTTTTACTCCCTTT
TTGCTAATTGTAGAATTCCT

Product: microcin C7 self-immunity protein (MccF)

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLAKGDLIGIICCSDGRKKEDEKDLKRLKQVLEKEFGLQVVFAKTIFQIDDSPFNGTPEERAAELMKLYQNMDVKMIFDI
SGGDAANQVLPYLNFNIIKKAAKPFIGYSDLTVILNAIFAKTKQPGFNYLLRNLVGEASEIQRVQFQKTFFENQIAINGK
SLNEFEWSLGEIVGGNIRCFLKLAGTEFMPDVSNKIILLESLGGKEAKIASYVAQLEQLGVFSKCLGIVVGEHTEAEKNG
EYDRIGILYQQIGLKYKLPVFRTKEIGHSSEAKPCLIGTKVNVSRETLTNF

Sequences:

>Translated_291_residues
MLAKGDLIGIICCSDGRKKEDEKDLKRLKQVLEKEFGLQVVFAKTIFQIDDSPFNGTPEERAAELMKLYQNMDVKMIFDI
SGGDAANQVLPYLNFNIIKKAAKPFIGYSDLTVILNAIFAKTKQPGFNYLLRNLVGEASEIQRVQFQKTFFENQIAINGK
SLNEFEWSLGEIVGGNIRCFLKLAGTEFMPDVSNKIILLESLGGKEAKIASYVAQLEQLGVFSKCLGIVVGEHTEAEKNG
EYDRIGILYQQIGLKYKLPVFRTKEIGHSSEAKPCLIGTKVNVSRETLTNF
>Mature_291_residues
MLAKGDLIGIICCSDGRKKEDEKDLKRLKQVLEKEFGLQVVFAKTIFQIDDSPFNGTPEERAAELMKLYQNMDVKMIFDI
SGGDAANQVLPYLNFNIIKKAAKPFIGYSDLTVILNAIFAKTKQPGFNYLLRNLVGEASEIQRVQFQKTFFENQIAINGK
SLNEFEWSLGEIVGGNIRCFLKLAGTEFMPDVSNKIILLESLGGKEAKIASYVAQLEQLGVFSKCLGIVVGEHTEAEKNG
EYDRIGILYQQIGLKYKLPVFRTKEIGHSSEAKPCLIGTKVNVSRETLTNF

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 32535; Mature: 32535

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKGDLIGIICCSDGRKKEDEKDLKRLKQVLEKEFGLQVVFAKTIFQIDDSPFNGTPEE
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEHHHHHCCCCCCCCCHHH
RAAELMKLYQNMDVKMIFDISGGDAANQVLPYLNFNIIKKAAKPFIGYSDLTVILNAIFA
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KTKQPGFNYLLRNLVGEASEIQRVQFQKTFFENQIAINGKSLNEFEWSLGEIVGGNIRCF
HCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCEEEE
LKLAGTEFMPDVSNKIILLESLGGKEAKIASYVAQLEQLGVFSKCLGIVVGEHTEAEKNG
EEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EYDRIGILYQQIGLKYKLPVFRTKEIGHSSEAKPCLIGTKVNVSRETLTNF
CCHHHHHHHHHHCCEEECCCEEECCCCCCCCCCCEEEECCCCCCHHHHCCC
>Mature Secondary Structure
MLAKGDLIGIICCSDGRKKEDEKDLKRLKQVLEKEFGLQVVFAKTIFQIDDSPFNGTPEE
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEHHHHHCCCCCCCCCHHH
RAAELMKLYQNMDVKMIFDISGGDAANQVLPYLNFNIIKKAAKPFIGYSDLTVILNAIFA
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KTKQPGFNYLLRNLVGEASEIQRVQFQKTFFENQIAINGKSLNEFEWSLGEIVGGNIRCF
HCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHCCCCEEEE
LKLAGTEFMPDVSNKIILLESLGGKEAKIASYVAQLEQLGVFSKCLGIVVGEHTEAEKNG
EEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EYDRIGILYQQIGLKYKLPVFRTKEIGHSSEAKPCLIGTKVNVSRETLTNF
CCHHHHHHHHHHCCEEECCCEEECCCCCCCCCCCEEEECCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]