Click here to switch to the map view.

The map label for this gene is manZ [H]

Identifier: 226222664

GI number: 226222664

Start: 28726

End: 29547

Strand: Direct

Name: manZ [H]

Synonym: Lm4b_00027

Alternate gene names: 226222664

Gene position: 28726-29547 (Clockwise)

Preceding gene: 226222663

Following gene: 226222665

Centisome position: 0.99

GC content: 39.78

Gene sequence:

>822_bases
ATGGCGAATACGAAGACTGAGCAGGATTTTGAAAAAGTACTCAAGAGACGCGATTTAATAGCAGCAAACTTTCGTTGGTT
GTTTGCCAGCCAAATATGTTGGAACTATGAGCGAATGATGTCGACTGGTTATCTTTATAGTATTTTGCCAACGCTTCGGA
AACTTTATAAAACAGACGATGATTTAAAAGATATGATGAATATGCATAACCAATTTTTCAATACGAATCCTATGGTTGGT
GGTTTGATCTTAGGAATGGATATGGCGATTGAAGAGCGCGAAAAGAAAGCGTCAAAAGAAGTCGTAACTGGTTTGAAAAC
GGGTTTGATGGGACCATTTGCTGGTGTTGGTGATACGATTTTCGGGGTTATTTTACCGACGATTTTTGGATCAATTGCCG
CATATATGGGACTTCAAGGTAATGTGACAGGTGTGGTTATCTGGGTATTAGTTAATATTTTGGTTGTGGGAGCAAGATTC
ACCTTACTTCCTCTAGGATATAAGCAAGGTGCAAAACTAGTGACGGAATTTGCGGACAGGCTTAACGCGTTAACCGATGC
AGCGATTCTGCTCGGGGTCACGGTCGTCGGGGCATTAATACCAACCGTAATCAAAGCAACTGTCCCATTCGTTTATAAAT
CAGGAAAAGTAGAGCTCAAAATGCAAGACATGCTCGATCAAATCATGCCATCACTCGTTCCAGTTTTACTCGTGGCGCTA
ATCTATGCACTTTTAGGCCACAAAAAAATGACATCTACGAAGGCTATTTTATTAGTAATGGTTATTGCGATTATTTTATT
CAACCTAAAAATTCTAGGTTAA

Upstream 100 bases:

>100_bases
ATTGGCGTGGCTTTGGCAATCATTTACTTTAAACAAAATTTCAAAAATCCAGTGGCTGCCGGAGCAAATGGCGCAGCGCT
AGTTGGGGAGGAGAATGAAG

Downstream 100 bases:

>100_bases
AATATAAAAATTGGAGGAATAGACAAAATGGGTTTAACATTTTTTGACAAAGCGCGTGAATTAACGGAGGAACTAGAAAA
AACACAGGCGGAAAGTATTC

Product: PTS system, mannose-specific IID component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MANTKTEQDFEKVLKRRDLIAANFRWLFASQICWNYERMMSTGYLYSILPTLRKLYKTDDDLKDMMNMHNQFFNTNPMVG
GLILGMDMAIEEREKKASKEVVTGLKTGLMGPFAGVGDTIFGVILPTIFGSIAAYMGLQGNVTGVVIWVLVNILVVGARF
TLLPLGYKQGAKLVTEFADRLNALTDAAILLGVTVVGALIPTVIKATVPFVYKSGKVELKMQDMLDQIMPSLVPVLLVAL
IYALLGHKKMTSTKAILLVMVIAIILFNLKILG

Sequences:

>Translated_273_residues
MANTKTEQDFEKVLKRRDLIAANFRWLFASQICWNYERMMSTGYLYSILPTLRKLYKTDDDLKDMMNMHNQFFNTNPMVG
GLILGMDMAIEEREKKASKEVVTGLKTGLMGPFAGVGDTIFGVILPTIFGSIAAYMGLQGNVTGVVIWVLVNILVVGARF
TLLPLGYKQGAKLVTEFADRLNALTDAAILLGVTVVGALIPTVIKATVPFVYKSGKVELKMQDMLDQIMPSLVPVLLVAL
IYALLGHKKMTSTKAILLVMVIAIILFNLKILG
>Mature_272_residues
ANTKTEQDFEKVLKRRDLIAANFRWLFASQICWNYERMMSTGYLYSILPTLRKLYKTDDDLKDMMNMHNQFFNTNPMVGG
LILGMDMAIEEREKKASKEVVTGLKTGLMGPFAGVGDTIFGVILPTIFGSIAAYMGLQGNVTGVVIWVLVNILVVGARFT
LLPLGYKQGAKLVTEFADRLNALTDAAILLGVTVVGALIPTVIKATVPFVYKSGKVELKMQDMLDQIMPSLVPVLLVALI
YALLGHKKMTSTKAILLVMVIAIILFNLKILG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=280, Percent_Identity=32.5, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789529, Length=275, Percent_Identity=29.4545454545455, Blast_Score=127, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 30127; Mature: 29996

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANTKTEQDFEKVLKRRDLIAANFRWLFASQICWNYERMMSTGYLYSILPTLRKLYKTDD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DLKDMMNMHNQFFNTNPMVGGLILGMDMAIEEREKKASKEVVTGLKTGLMGPFAGVGDTI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
FGVILPTIFGSIAAYMGLQGNVTGVVIWVLVNILVVGARFTLLPLGYKQGAKLVTEFADR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
LNALTDAAILLGVTVVGALIPTVIKATVPFVYKSGKVELKMQDMLDQIMPSLVPVLLVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEHHHHHHHHHHHHHHHHHHHHH
IYALLGHKKMTSTKAILLVMVIAIILFNLKILG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]