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The map label for this gene is tktA [C]

Identifier: 225861947

GI number: 225861947

Start: 1989772

End: 1990629

Strand: Reverse

Name: tktA [C]

Synonym: SPT_2140

Alternate gene names: 225861947

Gene position: 1990629-1989772 (Counterclockwise)

Preceding gene: 225861948

Following gene: 225861946

Centisome position: 94.25

GC content: 34.62

Gene sequence:

>858_bases
ATGATTTTAAGTAAAAATAGAGAAGATGAGTTAAGAAAATTTGCGACTAACATCCGATTAAATACTCTTAGAACATTGAA
TCATCTTGGATTCGGACATTACGGAGGAAGTCTGTCTATCGTAGAAGTTTTAGCGGTGCTTTATGGTGAAATAATGCCAA
TGACTCCAGAAATATTTGCAGCACGAGATAGAGATTATTTCATATTATCAAAAGGTCATGGAGGACCAGCTCTATACAGT
ACACTCTATTTGAATGGTTTCTTTGACAAAGAATTCTTATATTCTTTAAATACAAATGGAACCAAATTACCGTCTCATCC
TGATAGAAATCTAACGCCAGGCATAGATATGACAACGGGCTCTTTAGGACAAGGAATTAGTGTTGCAACTGGACTTGCAT
ATGGTCAGAGAATAAGAAAGAGTCCCTTTTATACTTATGCTATTGTTGGAGATGGTGAGTTAAATGAAGGACAATGTTGG
GAGGCTATACAGTTTGCTTCTCATCAACAGTTATCCAACTTAATTGTATTTGTTGATGATAACAAAAAACAATTAGATGG
TTTTACAAAGGATATTTGTAATCCAGGTGATTTCGTAGAAAAATTTTCAGCATTTGGATTTGAATCCATTAGGGTCAAGG
GTTCAGATATTAGAGAAATTTATGAAGGGATTGTCCAATTAAAACAGTCAAATAATTCATCACCTAAGTGCATTGTATTA
GATACTATTAAAGGTCAAGGGGTTCAAGAGCTGGAAGAAATGAAATCCAATCATCATCTTCGCCCTACTGTAGAGGAGAA
ACAAATGTTAACTTCAGTTGTAGAAAGATTAAGTCAGGAATTGGAGGAAACAGAATGA

Upstream 100 bases:

>100_bases
TGGCTCACAAGCAGGCATTGTGATTGGTCTTGTTCTTATTCTAGCAGTTATGTTTGGAGTATCCTTTATTAAAAAGCCAT
CTGCAAAGGAGGAATAAGGG

Downstream 100 bases:

>100_bases
TGAGAAGTACGAAAGAATTACGGCATGTATATAGAGACTTCCTTTTAGAGGCTAATCAAAGTTCTTCTGATATAGTAGTC
TTAGAAGCCGATTTGTCAAG

Product: transketolase, thiamine disphosphate-binding subunit

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVLAVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYS
TLYLNGFFDKEFLYSLNTNGTKLPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIREIYEGIVQLKQSNNSSPKCIVL
DTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVERLSQELEETE

Sequences:

>Translated_285_residues
MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVLAVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYS
TLYLNGFFDKEFLYSLNTNGTKLPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIREIYEGIVQLKQSNNSSPKCIVL
DTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVERLSQELEETE
>Mature_285_residues
MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVLAVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYS
TLYLNGFFDKEFLYSLNTNGTKLPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIREIYEGIVQLKQSNNSSPKCIVL
DTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVERLSQELEETE

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=276, Percent_Identity=31.8840579710145, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI205277463, Length=285, Percent_Identity=30.5263157894737, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI4507521, Length=285, Percent_Identity=30.5263157894737, Blast_Score=133, Evalue=1e-31,
Organism=Homo sapiens, GI225637459, Length=258, Percent_Identity=25.5813953488372, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI225637463, Length=154, Percent_Identity=31.8181818181818, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI225637461, Length=154, Percent_Identity=31.8181818181818, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI48994911, Length=248, Percent_Identity=31.0483870967742, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788808, Length=272, Percent_Identity=31.6176470588235, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17539652, Length=269, Percent_Identity=32.7137546468402, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6325331, Length=245, Percent_Identity=35.9183673469388, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319593, Length=244, Percent_Identity=32.3770491803279, Blast_Score=108, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24666278, Length=251, Percent_Identity=35.0597609561753, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45551847, Length=254, Percent_Identity=32.6771653543307, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI45550715, Length=254, Percent_Identity=32.6771653543307, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645119, Length=199, Percent_Identity=36.6834170854271, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 31956; Mature: 31956

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVLAVLYGEIMPMTPEIFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHE
ARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTKLPSHPDRNLTPGIDMTTG
ECCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCC
SLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCWEAIQFASHQQLSNLIVFVDD
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEC
NKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIREIYEGIVQLKQSNNSSPKCIVL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEE
DTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVERLSQELEETE
ECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVLAVLYGEIMPMTPEIFA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHE
ARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTKLPSHPDRNLTPGIDMTTG
ECCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHEEECCCCCCCCCCCCCCCCCCCCCCCC
SLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCWEAIQFASHQQLSNLIVFVDD
CCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEC
NKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIREIYEGIVQLKQSNNSSPKCIVL
CHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEE
DTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVERLSQELEETE
ECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]