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The map label for this gene is hisS [H]

Identifier: 225861939

GI number: 225861939

Start: 1982532

End: 1983821

Strand: Reverse

Name: hisS [H]

Synonym: SPT_2132

Alternate gene names: 225861939

Gene position: 1983821-1982532 (Counterclockwise)

Preceding gene: 225861940

Following gene: 225861938

Centisome position: 93.92

GC content: 46.98

Gene sequence:

>1290_bases
ATGAAATTACAAAAACCAAAAGGAACGCAGGATATTTTACCTGCTGAGTCTGCTAAGTGGCAGTACGTTGAGGGCTTTGC
CCGTGAAATTTTCAAGCGCTACAACTACGCAGAAGTGCGTACGCCTATTTTCGAGCATTACGAGGTTATCAGCCGCTCTG
TCGGAGATACAACGGATATCGTAACCAAGGAAATGTACGATTTCTATGACAAGGGTGACCGCCATATTACCCTCCGTCCA
GAAGGAACTGCGCCCGTTGTCCGTTCCTATGTGGAAAATAAACTCTTCGCCCCAGAAGTGCAAAAGCCAAGCAAGTTCTA
CTACATGGGACCTATGTTCCGTTATGAGCGTCCACAGGCAGGGCGCTTGCGCCAATTCCACCAGATTGGTGTTGAGTGTT
TTGGCTCTAGCAATCCAGCTACCGATGTGGAAACAATCGTTATGGCAGCCCATTTTTTGAAGGAAATCGGTATTCAAGGT
GTCAAATTGCACCTCAACACTCTTGGAAATCCTGAGAGCCGTGCAGCCTACCGCCAAGCCTTGATTGACTATTTGACACC
GCTCAAGGAGACCTTGTCTAAGGATAGCCAACGTCGCTTGGAGGAAAATCCTCTTCGTGTCTTGGACTCTAAGGAAAAAG
AAGACAAGGTGGCAGTAGAGAATGCGCCGTCTATCTTGGACTTTCTTGATGAAGAAAGCCAAACTCATTTTGATGCTGTG
CGTCAGATGTTGGAAAATCTTGGAGTAGATTACATCATCGATACCAATATGGTGCGTGGTCTGGACTACTACAACCACAC
CATTTTCGAGTTTATCACAGAGATTGAGGGCAATGACTTGACAGTCTGTGCGGGTGGTCGCTACGATGGTTTGGTTGCTT
ACTTTGGAGGCCCTGAAACTGCTGGATTTGGTTTTGGGCTTGGTGTAGAGCGCCTGCTTCTCATCCTTGAAAAACAAGGC
GTGGCCCTCCCTATCGAAAACGCCCTAGATGTCTATATCGCAGTCTTGGGTGATGGAGCAAATGTCAAAGCCCTAGAACT
AGTCCAAGCTCTTCGCCAACAAGGTTTCAAAGCAGAGCGTGATTACCTCAACCGTAAGCTCAAAGCTCAGTTCAAGTCAG
CAGATGTCTTTGCGGCTAAGACCCTCATCACCCTAGGAGAGAGCGAAGTCGAAAGCGGACAAGTGACGGTCAAGAACAAC
CAAACCCGAGAAGAAGTGCAAGTGTCACTTGAGACAATCAGCCAAAACTTCTCAGAAATCTTTGAAAAACTAGGATTTTA
TACTCAATGA

Upstream 100 bases:

>100_bases
TTAATCTCAAAAATGATATCTAAATCCTAATTTTTTTCTCACTGTTTTAATCCCTCTATTTATGGTAAAATAAGACTATT
AAGTTTAAAGAGGATTCCCT

Downstream 100 bases:

>100_bases
AAATCAAAGAGCAAACTAGGAAGCTAGCCGCAGGTTGCTCAAAACACTGTTTTGAGGTTGTAGATGAAACTGACGAAGTC
AGTAACCATACCTACGGCAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ

Sequences:

>Translated_429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ
>Mature_429_residues
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRP
EGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQG
VALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNN
QTREEVQVSLETISQNFSEIFEKLGFYTQ

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=478, Percent_Identity=25.7322175732218, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI6996014, Length=473, Percent_Identity=26.8498942917548, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=41.2322274881517, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71993693, Length=460, Percent_Identity=26.9565217391304, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71993686, Length=460, Percent_Identity=26.9565217391304, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325290, Length=472, Percent_Identity=21.8220338983051, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24643061, Length=465, Percent_Identity=25.3763440860215, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24643059, Length=484, Percent_Identity=24.5867768595041, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48655; Mature: 48655

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCH
GRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQGVKLHLNTLGNPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
LIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
HHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHH
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCC
AGFGFGLGVERLLLILEKQGVALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAER
CCCCCCHHHHHHHHHHHCCCCEECCCHHCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
DYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNNQTREEVQVSLETISQNFSEI
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
FEKLGFYTQ
HHHHCCCCC
>Mature Secondary Structure
MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIFEHYEVISRSVGDTTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHCCCHHHH
VTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQKPSKFYYMGPMFRYERPQA
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCH
GRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQGVKLHLNTLGNPESRAAYRQA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHH
LIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVAVENAPSILDFLDEESQTHFDAV
HHHHHHHHHHHHCCHHHHHHHCCCHHHHCCCCCCCCHHHCCCCHHHHHHCCCCHHHHHHH
RQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIEGNDLTVCAGGRYDGLVAYFGGPET
HHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCC
AGFGFGLGVERLLLILEKQGVALPIENALDVYIAVLGDGANVKALELVQALRQQGFKAER
CCCCCCHHHHHHHHHHHCCCCEECCCHHCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHH
DYLNRKLKAQFKSADVFAAKTLITLGESEVESGQVTVKNNQTREEVQVSLETISQNFSEI
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
FEKLGFYTQ
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA