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The map label for this gene is nusB

Identifier: 225858284

GI number: 225858284

Start: 450362

End: 450784

Strand: Reverse

Name: nusB

Synonym: SP70585_0502

Alternate gene names: 225858284

Gene position: 450784-450362 (Counterclockwise)

Preceding gene: 225858285

Following gene: 225858267

Centisome position: 20.63

GC content: 42.55

Gene sequence:

>423_bases
ATGACTAGTCCACTATTAGAATCTAGACGCCAACTCCGTAAATGCGCTTTTCAAGCTCTCATGAGCCTTGAGTTCGGTAC
GAATGTCGAAACTGCTTGTCGTTTCGCCTATACTCATGATCGTGAAGATACGGATGTACAACTTCCAGCCTTTTTGATAG
ACCTCGTTTCTGGTGTTCAAGCTAAAAAGGAAGAACTAGATAAGCAAATCACTCAGCATTTAAAAGTAGGTTGGACCATT
GAACGCTTAACGCTCGTGGAGAGAAACCTCCTTCGCTTGGGAGTTTTTGAAATCACTTCATTTGACACTCCTCAGCTGGT
TGCTGTTAATGAAGCTATCGAGCTTGCAAAGGACTTCTCCGATCAAAAATCTGCCCGTTTTATCAATGGACTGCTCAGCC
AGTTTGTAACAGAAGAACAATAA

Upstream 100 bases:

>100_bases
GATGTAGAACTCGCTGCTATCAATATTCACGTTGCAGGTATCGTCCCAGATAAAACACCAAAACCAGAATTGAAAGATCT
ATTTGACGAGGACTTCCTCA

Downstream 100 bases:

>100_bases
AAAGAAAAAGTGTTTGATATCTATCAAACACTTTTTCTTTGCCTCCCACTATCTAAAAAAATGATAATAGATATAATTGT
AAACAAAAATCCAGATAGGT

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDTDVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTI
ERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ

Sequences:

>Translated_140_residues
MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDTDVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTI
ERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ
>Mature_139_residues
TSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDTDVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIE
RLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=124, Percent_Identity=34.6774193548387, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 15991; Mature: 15860

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDTDVQLPAFLIDLVSGVQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCH
AKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DQKSARFINGLLSQFVTEEQ
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDTDVQLPAFLIDLVSGVQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCH
AKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DQKSARFINGLLSQFVTEEQ
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA