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The map label for this gene is yclM [H]

Identifier: 225858266

GI number: 225858266

Start: 436944

End: 438308

Strand: Reverse

Name: yclM [H]

Synonym: SP70585_0484

Alternate gene names: 225858266

Gene position: 438308-436944 (Counterclockwise)

Preceding gene: 225858267

Following gene: 225858265

Centisome position: 20.06

GC content: 42.27

Gene sequence:

>1365_bases
ATGAAAGTTGTTAAATTTGGAGGTAGTTCTCTTGCCTCTGCTAGTCAATTAGAAAAAGTTTTAAACATCGTCAAAAGCGA
TTCAGAGCGTCGTTTTGTAGTCGTTTCTGCGCCTGGTAAACGCAATGCTGAAGATACTAAGGTTACGGATGCCCTGATTA
AATACTACCGCGACTATGTTGCGGGTAACGATATTAGCAAGAGCCAAAGCTGGATTATCGACCGCTATGCTGCTATGGTT
AGTGAATTGGGACTAAAACCAGCTGTGCTAGAAAAAATTTCTAAAAGTATTCACGCCTTGGCCACTCTTCCTATTGAAGA
AAATGAATTTCTCTACGATACTTTCCTAGCAGCCGGTGAAAATAACAATGCCAAATTGATTGCTGCCTACTTTAACCAAA
ATGGTATCGATGCACGCTATATGCACCCTAGAGAAGCTGGGATTGTGGTCACAAGTGAACCTGGTCACGCTCGCATCATT
CCATCAAGTTATGACAAGATTGAAGAATTGACAAACACCAATGAAGTCCTTGTCATTCCTGGTTTCTTTGGTGTCACTAA
GGAAAATCAAATCTGTACTTTCTCACGTGGAGGTTCTGATATTACAGGTTCTATCATTGCTGCTGGTGTCAAAGCTGACC
TCTATGAAAACTTTACGGACGTTGATGGTATCTTTGCAGCTCACCCTGGTATTATCCACCAACCACACTCGATTCCTGAG
TTGACCTACCGTGAAATGCGCGAGTTGGCCTATGCAGGCTTCTCAGTCCTTCATGACGAGGCTCTTCTTCCTGCCTACCG
TGGAAAAATTCCTCTGGTTATCAAGAATACCAACAACCCTGACCATCCAGGTACTCGTATCGTTCTAAAACACAGTAATG
ATGAATTTCCAGTTGTGGGAATTGCTGGTGACTCAGGCTTTGTCAGCATTAACATGTCGAAATACCTCATGAACCGTGAG
GTTGGATTTGGCCGCAAGGTTCTGCAAATCCTGGAAGAACTTAACATCGGTTGGGAACATATGCCAACAGGTATCGACGA
TCTTTCTATCATTCTCCGTTCTCGCCAACTAACTCCCATCAAGAAAGAAGAAATCCTGCGTCAGTTGGTTCAAAAGGCTA
AAGTAGATCATGCAGAAATCGAACACGACCTTTCTATCATTATGATTGTTGGTGAAAAAATGAAGAGCCATATCGGAGTG
ACTGCTACTGCGACACGCGCTCTATCTGAAAACAAAATCAACATCCAGATGATGTCTCAAGGTTCTAGTGAAGTTTCTAT
TATGTTTGTTGTCAATAAAGACCAAGAGAAAGCAGCAATTAAAGCCCTCTACAATGCCTTTTTTGGAGAAAGTAAGGAAG
ACTAA

Upstream 100 bases:

>100_bases
AGAAAGGCTCGCTTTAGGCTAGAAAGCATCTTATTTTCTTCATTGAAAAAAGCCTTTCTTTCTGCTATAATCGTATAAAA
TACTTACTTTAGGAGTTCTT

Downstream 100 bases:

>100_bases
GCATGGCTCAATCACTTAACAAAACAGTACTCCTCAGTACGACAGGCACTTCCTACCTCTCTATAGCTGGGAAAGTTGGG
AAATTCCTTGTCGGAGATCA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAEDTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMV
SELGLKPAVLEKISKSIHALATLPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENFTDVDGIFAAHPGIIHQPHSIPE
LTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKNTNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNRE
VGFGRKVLQILEELNIGWEHMPTGIDDLSIILRSRQLTPIKKEEILRQLVQKAKVDHAEIEHDLSIIMIVGEKMKSHIGV
TATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGESKED

Sequences:

>Translated_454_residues
MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAEDTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMV
SELGLKPAVLEKISKSIHALATLPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENFTDVDGIFAAHPGIIHQPHSIPE
LTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKNTNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNRE
VGFGRKVLQILEELNIGWEHMPTGIDDLSIILRSRQLTPIKKEEILRQLVQKAKVDHAEIEHDLSIIMIVGEKMKSHIGV
TATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGESKED
>Mature_454_residues
MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAEDTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMV
SELGLKPAVLEKISKSIHALATLPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENFTDVDGIFAAHPGIIHQPHSIPE
LTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKNTNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNRE
VGFGRKVLQILEELNIGWEHMPTGIDDLSIILRSRQLTPIKKEEILRQLVQKAKVDHAEIEHDLSIIMIVGEKMKSHIGV
TATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGESKED

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=473, Percent_Identity=25.7928118393235, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1790455, Length=463, Percent_Identity=24.8380129589633, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790376, Length=298, Percent_Identity=26.510067114094, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320893, Length=390, Percent_Identity=26.1538461538462, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50208; Mature: 50208

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAEDTKVTDALIKYYRDYV
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
AGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALATLPIEENEFLYDTFLAAGE
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCEEEEHHHCCCC
NNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARIIPSSYDKIEELTNTNEVLVIP
CCCCEEEEEEECCCCCCEEEECCCCCCEEEECCCCCEEECCCHHHHHHHHCCCCCEEEEC
GFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENFTDVDGIFAAHPGIIHQPHSIPE
CCCCCCCCCCEEEECCCCCCCCCHHHEECCHHHHHHCCCCCCCEEECCCCCCCCCCCCCH
LTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKNTNNPDHPGTRIVLKHSNDEFPVVG
HHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEE
IAGDSGFVSINMSKYLMNREVGFGRKVLQILEELNIGWEHMPTGIDDLSIILRSRQLTPI
EECCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCC
KKEEILRQLVQKAKVDHAEIEHDLSIIMIVGEKMKSHIGVTATATRALSENKINIQMMSQ
CHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEHHHHHHCCEEEHHHHHHCCCCEEEEEEEC
GSSEVSIMFVVNKDQEKAAIKALYNAFFGESKED
CCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAEDTKVTDALIKYYRDYV
CEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
AGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALATLPIEENEFLYDTFLAAGE
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCCEEEEHHHCCCC
NNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARIIPSSYDKIEELTNTNEVLVIP
CCCCEEEEEEECCCCCCEEEECCCCCCEEEECCCCCEEECCCHHHHHHHHCCCCCEEEEC
GFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENFTDVDGIFAAHPGIIHQPHSIPE
CCCCCCCCCCEEEECCCCCCCCCHHHEECCHHHHHHCCCCCCCEEECCCCCCCCCCCCCH
LTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKNTNNPDHPGTRIVLKHSNDEFPVVG
HHHHHHHHHHHHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEE
IAGDSGFVSINMSKYLMNREVGFGRKVLQILEELNIGWEHMPTGIDDLSIILRSRQLTPI
EECCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCCCC
KKEEILRQLVQKAKVDHAEIEHDLSIIMIVGEKMKSHIGVTATATRALSENKINIQMMSQ
CHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEHHHHHHCCEEEHHHHHHCCCCEEEEEEEC
GSSEVSIMFVVNKDQEKAAIKALYNAFFGESKED
CCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]