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The map label for this gene is ribH

Identifier: 225858036

GI number: 225858036

Start: 209218

End: 209685

Strand: Reverse

Name: ribH

Synonym: SP70585_0238

Alternate gene names: 225858036

Gene position: 209685-209218 (Counterclockwise)

Preceding gene: 225858037

Following gene: 225858033

Centisome position: 9.6

GC content: 35.68

Gene sequence:

>468_bases
ATGAACACTTATGAAGGTAATTTAGTAGCAAACAATATTAAAATAGGTATTGTTGTAGCGAGATTTAATGAATTTATAAC
TTCAAAATTATTATCTGGAGCACTAGATAATCTCAAAAGAGAGAATGTAAACGAGAAAGATATAGAGGTAGCCTGGGTTC
CAGGAGCTTTTGAAATACCACTGATTGCATCAAAAATGGCAAAAAGTAAAAAATATGATGCAATTATCTGCTTGGGAGCT
GTCATTAGAGGGAATACAAGTCATTATGATTATGTATGTAGCGAGGTATCTAAGGGAATCGCCCAAATCAGTTTAAATAG
CGAAATTCCTGTTATGTTTGGTGTGCTAACGACAGATACAATTGAACAAGCCATAGAACGAGCTGGTACTAAAGCAGGAA
ATAAGGGTTCTGAGTGTGCACAAGGAGCTATTGAAATGGTCAACCTGATTCGTACATTAGACGCATAG

Upstream 100 bases:

>100_bases
TAGAATATCAATCGAAATAGAAGCCAATCCTTACGATAGTTTTTATTTAGAAACAAAGAAAAATCGAATGGGTCACATTT
TAAATATGGAGGAAAAATAA

Downstream 100 bases:

>100_bases
AACAGACTCTACTTTTAGACGAATAAAGAAACTTGGCAAAATCGCAATTTAATACTCTGTGAAAATCATAGAGCAAACTA
GGAAACCAGTCGCAGAGTGG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVNEKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGA
VIRGNTSHYDYVCSEVSKGIAQISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA

Sequences:

>Translated_155_residues
MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVNEKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGA
VIRGNTSHYDYVCSEVSKGIAQISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA
>Mature_155_residues
MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVNEKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGA
VIRGNTSHYDYVCSEVSKGIAQISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=156, Percent_Identity=54.4871794871795, Blast_Score=183, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324429, Length=154, Percent_Identity=31.8181818181818, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_STRP2 (Q04MR3)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815685.1
- ProteinModelPortal:   Q04MR3
- SMR:   Q04MR3
- STRING:   Q04MR3
- EnsemblBacteria:   EBSTRT00000019807
- GeneID:   4442376
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0166
- eggNOG:   COG0054
- GeneTree:   EBGT00050000027635
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16752; Mature: 16752

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVNEKDIEVAWVPGAFEIP
CCCCCCCEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
LIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQISLNSEIPVMFGVLTTDT
HHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHEEEECCCCCCEEEEHHHHHH
IEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVNEKDIEVAWVPGAFEIP
CCCCCCCEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
LIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQISLNSEIPVMFGVLTTDT
HHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHEEEECCCCCCEEEEHHHHHH
IEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA