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The map label for this gene is rnjA [H]

Identifier: 225858001

GI number: 225858001

Start: 176750

End: 178429

Strand: Reverse

Name: rnjA [H]

Synonym: SP70585_0201

Alternate gene names: 225858001

Gene position: 178429-176750 (Counterclockwise)

Preceding gene: 225858002

Following gene: 225857984

Centisome position: 8.17

GC content: 46.55

Gene sequence:

>1680_bases
ATGGCCTACACTCTTAAACCTGAAGAAGTCGGCGTTTTTGCCATCGGTGGTCTAGGAGAAATCGGGAAAAACACTTACGG
AATTGAATACCAAGACGAGATTATCATCGTCGATGCTGGGATTAAATTCCCAGAAGATGACTTGCTTGGTATCGACTATG
TCATTCCTGACTACTCTTACATCGTGGACAATATCGACCGCGTCAAGGCTGTTTTAATCACACACGGACACGAGGACCAC
ATTGGTGGGATTCCGTTCCTACTCAAGCAAGCAAATGTCCCTATCTACGCTGGACCGCTTGCCTTGGCTTTGATCCGTGG
GAAACTCGAAGAACACGGCCTCTTGCGCAACGCCAAACTTTACGAAATCAACCACAACACCGAGTTGACCTTTAAAAATC
TCAAGGCAACTTTCTTTAGAACGACTCACTCTATTCCAGAGCCTTTGGGGATTGTCATTCATACTCCTCAAGGGAAAATC
GTCTGTACGGGTGACTTTAAGTTCGACTTTACTCCAGTTGGAGAACCTGCGGACTTGCATCGTATGGCTGCGCTTGGTGA
AGAAGGCGTGCTCTGTCTCCTGTCTGACTCGACAAATGCGGAAGTACCAACCTTTACCAACTCTGAAAAAGTCGTTGGTC
AGTCCATTATGAAGATTATCCAAGGTATTGAAGGACGTATCATCTTTGCATCCTTTGCCTCAAATATCTTCCGTCTCCAG
CAGGCAACAGAAGCTGCTGTTAAGACTGGACGCAAGATTGCGGTCTTTGGTCGTTCTATGGAAAAGGCCATTGTCAACGG
AATCGATCTTGGCTACATCAAAGCTCCTAAGGGAACCTTTATCGAGCCAAATGAAATCAAAGATTATCCTGCAGGAGAAG
TTCTTATCCTCTGTACAGGTAGTCAGGGTGAGCCTATGGCAGCCCTCTCTCGTATCGCCAACGGAACCCACCGTCAAGTA
CAATTACAACCAGGTGATACCGTTATCTTCTCTTCTAGTCCCATCCCTGGAAACACTACTAGCGTCAACAAGCTGATTAA
CATCATTTCTGAAGCTGGTGTCGAAGTTATCCACGGTAAAGTGAACAATATCCATACATCTGGACACGGTGGTCAGCAAG
AGCAAAAACTCATGCTCTGCTTGATTAAGCCAAAATACTTCATGCCTGTCCACGGTGAATACCGCATGCAAAAAGTCCAC
GCTGGACTAGCAGTGGATACTGGTGTTGAGAAGGACAATATCTTTATCATGAGCAATGGCGATGTGCTTGCCCTTACTGC
TGACTCAGCTCGTATCGCAGGTCATTTCAACGCCCAAGATATCTATGTCGATGGAAATCGTATCGGTGAAATTGGCGCAG
CTGTCCTCAAAGATCGTCGCGATCTATCTGAAGACGGTGTCGTTCTGGCAGTTGCAACTGTTGACTTCAAATCGCAGATG
ATTCTATCTGGTCCAGACATCCTCAGCCGAGGCTTTGTCTACATGAGAGAGTCTGGCGACTTGATTCGCCAAAGCCAGCG
TATCCTCTTCAATGCCATTCGTATCGCACTGAAAAATAAGGATGCTAGCGTGCAATCTGTCAATGGTGCCATTGTCAACG
CTATTCGCCCCTTCCTCTATGAAAATACCGAACGTGAACCGATCATCATCCCGATGATCCTCACACCAGATGAAGAATAA

Upstream 100 bases:

>100_bases
AATCGCCCAGAGTACAATATCGAGTATATCGAACTCTTGTCTGACAAATTGCTCGATTACGAAAAAGAAACTGGCGCCTT
CGAAATTACGGAGTTCTAAT

Downstream 100 bases:

>100_bases
TACTCTTCAAACCACGTCAGCTTCGCCTTGCCGTATATATGTTACTGACTTCGTCAATTCTATCCACAACCTCAAAACTG
TGTTTTGAGCTGACTTCGTC

Product: metallo-beta-lactamase superfamily protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDH
IGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQV
QLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHGEYRMQKVH
AGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE

Sequences:

>Translated_559_residues
MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDH
IGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQV
QLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHGEYRMQKVH
AGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE
>Mature_558_residues
AYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHI
GGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKIV
CTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQQ
ATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQ
LQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHGEYRMQKVHA
GLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQMI
LSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61010; Mature: 60879

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSY
CCEECCCCCCCEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCHHH
IVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKL
HHCCHHHEEEEEEECCCCCCCCCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCEE
YEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKIVCTGDFKFDFTPVGEPADLH
EEECCCCEEEEECCEEEEEEECCCCCCCCEEEEECCCCCEEEEECCEEEECCCCCCHHHH
RMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTG
HHHHHHHHCCCEEEEECCCHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEC
SQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGK
CCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECC
VNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHGEYRMQKVHAGLAVDTGVEKDNIFIMSNG
CCEEEECCCCCCCCCEEEEEEECCCEEECCCCCHHEEHHHCCEEEECCCCCCCEEEEECC
DVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
CEEEEEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEE
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLY
EECCCCHHHCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCEECCCCHHHHHHHHHHH
ENTEREPIIIPMILTPDEE
CCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
AYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSY
CEECCCCCCCEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCHHH
IVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKL
HHCCHHHEEEEEEECCCCCCCCCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCEE
YEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKIVCTGDFKFDFTPVGEPADLH
EEECCCCEEEEECCEEEEEEECCCCCCCCEEEEECCCCCEEEEECCEEEECCCCCCHHHH
RMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTG
HHHHHHHHCCCEEEEECCCHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEC
SQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGK
CCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECC
VNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHGEYRMQKVHAGLAVDTGVEKDNIFIMSNG
CCEEEECCCCCCCCCEEEEEEECCCEEECCCCCHHEEHHHCCEEEECCCCCCCEEEEECC
DVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
CEEEEEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEE
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLY
EECCCCHHHCCEEEEECCCHHHHHHHHHHHHHHEEEEECCCCCEECCCCHHHHHHHHHHH
ENTEREPIIIPMILTPDEE
CCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]