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The map label for this gene is cobS [H]

Identifier: 225852378

GI number: 225852378

Start: 860314

End: 861096

Strand: Reverse

Name: cobS [H]

Synonym: BMEA_A0904

Alternate gene names: 225852378

Gene position: 861096-860314 (Counterclockwise)

Preceding gene: 225852380

Following gene: 225852377

Centisome position: 40.51

GC content: 62.45

Gene sequence:

>783_bases
TTGCAGCGAAATGGCCTGATTGGCGACACGATCAGAAGTCTGGGATTTTTGAGCCGCCTGCCACTGCCGCAAGGCTGGTT
CGACAATACGGACGATTCGCTGCCGCGAAACGCACGCGCCTTTCCGCTTGCGGGCGGCATATTGGGGCTTCTGGCCGGCG
TGGCACTTCTCATTGCCAATGCAATCAGCCTGCCGCCGCTTGCGGCTGCACTGATTGCCATCGGTGCGCTGGCAGCAATG
ACGGGTGCGCTGCATGAAGATGGCCTGGGCGATACGGCCGATGGTTTTTTCGGTGCATCCACGCCAGATCGCCGCCTCGA
TATCATGAAAGATTCGCGCATCGGCACTTTCGCGGCCCTGACGCTGGTTATCTGGACCGGCGTGAAAGCGTCTCTCCTGA
TGGCAATCATCGCGCGCGCGGGCGCAGGCTATGCGCTTCTGGCCCTGATCGGGACGGAAGCAGCCAGCCGCGCAGGAATG
CTGGCCTTCTGGCATGCCCTGCCCTCGGCGCGCCCCGGCGGGCTTGCCGATAGTATGGGCCAGCCGCAATGGGAAACCGT
GGTCTGCGGCTGCGGGCTGGGTCTTGCTCTCCTCGCCATCGGCTTTCTGCCGTCAGGCGGCATGGTGGCCCTCATCAACG
CACTGGTGCTGATGACCGTCGTGCTTTTTGGTTTTGCCAGACTGTGCATGGCAAAAATCGGCGGACGGACCGGCGATACG
CTGGGTGCGGCGCAGCAGATAGGCTCGCTTGCAGCCCTTATCGGACTTGTCATGGCCCTTTGA

Upstream 100 bases:

>100_bases
AAGCACTGACGCGAATTTTCAAATCACCTCTTTGATCTGAAACAATATTGAGGCGAAATATCGCGATGGTTCGACCCTGA
AAACAAAGCGGAGCAGTGAT

Downstream 100 bases:

>100_bases
TGCAACCACATTGCATTTCATGGACACGGCAAAAATCGAATCGCCCTGCATATTGGTATGCACGATGGATGCAAAAACTG
GCTTCTGCCTCGGTTGCGCG

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIANAISLPPLAAALIAIGALAAM
TGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAALTLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGM
LAFWHALPSARPGGLADSMGQPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGRTGDT
LGAAQQIGSLAALIGLVMAL

Sequences:

>Translated_260_residues
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIANAISLPPLAAALIAIGALAAM
TGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAALTLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGM
LAFWHALPSARPGGLADSMGQPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGRTGDT
LGAAQQIGSLAALIGLVMAL
>Mature_260_residues
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIANAISLPPLAAALIAIGALAAM
TGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAALTLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGM
LAFWHALPSARPGGLADSMGQPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGRTGDT
LGAAQQIGSLAALIGLVMAL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=36.1788617886179, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26433; Mature: 26433

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIAN
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AISLPPLAAALIAIGALAAMTGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAAL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
TLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGMLAFWHALPSARPGGLADSMG
HHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCC
QPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGRTGDT
CCCHHHEEHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LGAAQQIGSLAALIGLVMAL
HHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MQRNGLIGDTIRSLGFLSRLPLPQGWFDNTDDSLPRNARAFPLAGGILGLLAGVALLIAN
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AISLPPLAAALIAIGALAAMTGALHEDGLGDTADGFFGASTPDRRLDIMKDSRIGTFAAL
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
TLVIWTGVKASLLMAIIARAGAGYALLALIGTEAASRAGMLAFWHALPSARPGGLADSMG
HHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCC
QPQWETVVCGCGLGLALLAIGFLPSGGMVALINALVLMTVVLFGFARLCMAKIGGRTGDT
CCCHHHEEHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LGAAQQIGSLAALIGLVMAL
HHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA