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The map label for this gene is purB [H]

Identifier: 225852361

GI number: 225852361

Start: 844181

End: 845482

Strand: Reverse

Name: purB [H]

Synonym: BMEA_A0887

Alternate gene names: 225852361

Gene position: 845482-844181 (Counterclockwise)

Preceding gene: 225852362

Following gene: 225852358

Centisome position: 39.77

GC content: 59.45

Gene sequence:

>1302_bases
ATGATCCCGCGCTATTCCCGGCCAGAAATGGTCGCCATCTGGTCGCCCGAAACGAAGTTCCGCATCTGGTTCGAGATCGA
AGCCTATGCCTGCGAGGCGCTGGCTGAACTGGGCGTCATCCCGAAGGAAGCGGCCAGGACCATCTGGGAAAAGGGCAGCG
TGGCGAAATTCGACGTCGCCCGCATCGACGAAATCGAAGCCGTCACCAAGCATGACGTGATCGCATTCCTCACGCATCTG
GCCGAATTTATCGGCCCTGACAGCCGTTTCGTCCATCAGGGCATGACGTCATCGGACGTGCTCGACACCACGCTCAATGT
CCAGCTTGTGCGCGCTGCCGACCTGCTGCTCGCTGATCTGGACCGCGTGCTGGAAGCCCTGAAGAAACGTGCTTTCGAGC
ACAAGGACACGGTGCGCATCGGCCGCAGCCACGGCATTCATGCCGAACCGACCACGATGGGCCTCACTTTCGCGCGTTTC
TATGCCGAAATGTCGCGCAATCGCGCCCGTCTCGTTGCCGCGCGCGCGGAAATCGCCACGGGCGCAATCTCGGGTGCCGT
CGGCACTTTCGCCAATATCGACCCGCGCGTGGAAGAATATGTCTGCGCCAAGCTCGGCCTTGAAGCAGAGCCCGTTTCGA
CACAGGTGATCCCGCGCGACCGCCACGCCATGTTTTTCGCAACGCTCGGCGTCATTGCATCCTCGATGGAAAACATCGCC
ATCGAAATTCGCCATATGCAGCGCACGGAAGTTCTGGAAGCGGAAGAATTCTTCTCGCCGGGCCAGAAGGGTTCGTCGGC
CATGCCGCACAAGCGCAATCCGGTGCTGACCGAAAACCTGACCGGCCTTGCCCGTCTGGTGCGCATGTCGGTCACGCCTG
CGATGGAAAATGTCGCTCTGTGGCACGAACGCGATATTTCGCATTCGAGCGTTGAGCGCGCTATTGGACCGGACACGACC
ATCACGCTCGATTTCGCGCTCAACCGTCTGGCCGGCGTGATCGAGAAGCTGGTGATCTATCCGGACAACATGCTCAAAAA
CATGAACAAGTTCCGCGGTCTGGTGCATTCGCAGCGCGTATTGTTGGCCCTAACGCAGGCCGGTGTGTCGCGTGAAGACG
CCTATCGTCTCGTGCAGCGCAACGCCATGAAGGTCTGGGAACAGAGCGTTGATTTCCTCGAAGAATTGCTGGCCGACACG
GAAGTGCGCGCTGCACTTTCCGAAGAGCAGATCCGCGAGAAGTTCGATCTCGGCTACCACACCAAGCATGTGGATACGAT
TTTCAAGCGCGTCTTCGGCTGA

Upstream 100 bases:

>100_bases
GTAATTCGGTCGAGAGTTACCGTGCCAATATCGACGCCATTCGTGCTGCGGCCGAAGCCGCGCGTAATCGTTAAACCCTC
ACTTTCCACAGGATGCACCC

Downstream 100 bases:

>100_bases
AGCAGCGCGGCCTACCCCTCCCTCACCCTGAGGAGCCGTTGGAAACGGCGCATCATCCTGAGCTTGTCGAAGGATCGAAG
GGCTGAGAGAAACACTCTGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAYACEALAELGVIPKEAARTIWEKGSVAKFDVARIDEIEAVTKHDVIAFLTHL
AEFIGPDSRFVHQGMTSSDVLDTTLNVQLVRAADLLLADLDRVLEALKKRAFEHKDTVRIGRSHGIHAEPTTMGLTFARF
YAEMSRNRARLVAARAEIATGAISGAVGTFANIDPRVEEYVCAKLGLEAEPVSTQVIPRDRHAMFFATLGVIASSMENIA
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVTPAMENVALWHERDISHSSVERAIGPDTT
ITLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQSVDFLEELLADT
EVRAALSEEQIREKFDLGYHTKHVDTIFKRVFG

Sequences:

>Translated_433_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAYACEALAELGVIPKEAARTIWEKGSVAKFDVARIDEIEAVTKHDVIAFLTHL
AEFIGPDSRFVHQGMTSSDVLDTTLNVQLVRAADLLLADLDRVLEALKKRAFEHKDTVRIGRSHGIHAEPTTMGLTFARF
YAEMSRNRARLVAARAEIATGAISGAVGTFANIDPRVEEYVCAKLGLEAEPVSTQVIPRDRHAMFFATLGVIASSMENIA
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVTPAMENVALWHERDISHSSVERAIGPDTT
ITLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQSVDFLEELLADT
EVRAALSEEQIREKFDLGYHTKHVDTIFKRVFG
>Mature_433_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAYACEALAELGVIPKEAARTIWEKGSVAKFDVARIDEIEAVTKHDVIAFLTHL
AEFIGPDSRFVHQGMTSSDVLDTTLNVQLVRAADLLLADLDRVLEALKKRAFEHKDTVRIGRSHGIHAEPTTMGLTFARF
YAEMSRNRARLVAARAEIATGAISGAVGTFANIDPRVEEYVCAKLGLEAEPVSTQVIPRDRHAMFFATLGVIASSMENIA
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVTPAMENVALWHERDISHSSVERAIGPDTT
ITLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRVLLALTQAGVSREDAYRLVQRNAMKVWEQSVDFLEELLADT
EVRAALSEEQIREKFDLGYHTKHVDTIFKRVFG

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=414, Percent_Identity=28.2608695652174, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.6089238845144, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1787376, Length=350, Percent_Identity=29.1428571428571, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=416, Percent_Identity=25.7211538461538, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=360, Percent_Identity=25.8333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323391, Length=386, Percent_Identity=27.2020725388601, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24647570, Length=381, Percent_Identity=25.7217847769029, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640177, Length=250, Percent_Identity=26.4, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24640179, Length=250, Percent_Identity=26.4, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI78710009, Length=209, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48682; Mature: 48682

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAYACEALAELGVIPKEAARTIWEKGSVAKFDVA
CCCCCCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHH
RIDEIEAVTKHDVIAFLTHLAEFIGPDSRFVHQGMTSSDVLDTTLNVQLVRAADLLLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DRVLEALKKRAFEHKDTVRIGRSHGIHAEPTTMGLTFARFYAEMSRNRARLVAARAEIAT
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEYVCAKLGLEAEPVSTQVIPRDRHAMFFATLGVIASSMENIA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVTPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
WHERDISHSSVERAIGPDTTITLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRV
HHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEQSVDFLEELLADTEVRAALSEEQIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFKRVFG
HHHHHHHHHHHCC
>Mature Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAYACEALAELGVIPKEAARTIWEKGSVAKFDVA
CCCCCCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHH
RIDEIEAVTKHDVIAFLTHLAEFIGPDSRFVHQGMTSSDVLDTTLNVQLVRAADLLLADL
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DRVLEALKKRAFEHKDTVRIGRSHGIHAEPTTMGLTFARFYAEMSRNRARLVAARAEIAT
HHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEYVCAKLGLEAEPVSTQVIPRDRHAMFFATLGVIASSMENIA
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHMQRTEVLEAEEFFSPGQKGSSAMPHKRNPVLTENLTGLARLVRMSVTPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
WHERDISHSSVERAIGPDTTITLDFALNRLAGVIEKLVIYPDNMLKNMNKFRGLVHSQRV
HHHCCCCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDAYRLVQRNAMKVWEQSVDFLEELLADTEVRAALSEEQIREKFDLGYH
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFKRVFG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]