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The map label for this gene is garL [H]

Identifier: 225852357

GI number: 225852357

Start: 841429

End: 842196

Strand: Direct

Name: garL [H]

Synonym: BMEA_A0883

Alternate gene names: 225852357

Gene position: 841429-842196 (Clockwise)

Preceding gene: 225852356

Following gene: 225852359

Centisome position: 39.58

GC content: 58.2

Gene sequence:

>768_bases
ATGTCAATGTCGTTGTCTTCGCGCCTTCGCGCCGGTGAGACCGTGCTTTCTGCCTGGTCATCCTTGCCGGAACCCCTGAC
TGTAGAAATTCTTGCGCATAGTGCTTTCGATGCCGTGACGCTGGACATGCAGCATGGCGGGCACGACGAGGCGAGCATTC
TGCGCAGCCTCGGCCTGGTGCTGAATGCAGGCAAGCCCCCAGTGGTGCGCATTCCTGTCGGACGCTTCGATATGGCAAGC
CGCGCGCTCGATTTTGGCGCCCATGCGGTGATTGCTCCGATGATAAATTCAGTCGATGACGCCCGCAGATTTGCGGCCGC
GATGAAATATCCGCCAGTTGGCGAGCGTTCATGGGGCGTGTTCCGGGCAAATGCGGATTATGGCGCGCCGGGTTCCAACG
ATTATCTGACCACCGCCAACCACGACACGCTTGCCTTCGCGATGATCGAGACGCGCGATGCCTATGATGCGCTGGATGCC
ATTCTGGATGTGCGCGGTATTGATGGCGTTTTTGTCGGCCCGTCCGATTTTTCCATCGCATGGAGCAACGGACGCGAGGC
GAACCCGCATTCGGATGCAATCGTGGAACCGATCAGCAATATTGCACACAAGGCCGCAGCAGCCGGCAAGATTGCAGGCA
TCTATTCGCCATCGCCGGAGTTTGCGCGCCGCTATATTTCGCTGGGTTTCCGGTTCCTGACAATTAGCAATGACAGCGGC
TATATCCGCCTTGCGGCGGAAACGCTGGTGAAAGCTGTTCGCGGATAA

Upstream 100 bases:

>100_bases
ATGGAAGAGGCGATCCGTCAGGTGAAGCAGGATTGATGATGATTTGGGGCGGGGGCACACAGTGTTTCCGCCCTGAACTT
GTGAAGTGAAGGAAATACGC

Downstream 100 bases:

>100_bases
TCAAACGGAAAAGGCAATGTTCCATGCCTGCCTTTTTGTTTCAGGCTCTCAATGCTGTTTGGGAGCCTGAAGCTGGGTCA
TGAATTCCGCAAAGACCATG

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSMSLSSRLRAGETVLSAWSSLPEPLTVEILAHSAFDAVTLDMQHGGHDEASILRSLGLVLNAGKPPVVRIPVGRFDMAS
RALDFGAHAVIAPMINSVDDARRFAAAMKYPPVGERSWGVFRANADYGAPGSNDYLTTANHDTLAFAMIETRDAYDALDA
ILDVRGIDGVFVGPSDFSIAWSNGREANPHSDAIVEPISNIAHKAAAAGKIAGIYSPSPEFARRYISLGFRFLTISNDSG
YIRLAAETLVKAVRG

Sequences:

>Translated_255_residues
MSMSLSSRLRAGETVLSAWSSLPEPLTVEILAHSAFDAVTLDMQHGGHDEASILRSLGLVLNAGKPPVVRIPVGRFDMAS
RALDFGAHAVIAPMINSVDDARRFAAAMKYPPVGERSWGVFRANADYGAPGSNDYLTTANHDTLAFAMIETRDAYDALDA
ILDVRGIDGVFVGPSDFSIAWSNGREANPHSDAIVEPISNIAHKAAAAGKIAGIYSPSPEFARRYISLGFRFLTISNDSG
YIRLAAETLVKAVRG
>Mature_254_residues
SMSLSSRLRAGETVLSAWSSLPEPLTVEILAHSAFDAVTLDMQHGGHDEASILRSLGLVLNAGKPPVVRIPVGRFDMASR
ALDFGAHAVIAPMINSVDDARRFAAAMKYPPVGERSWGVFRANADYGAPGSNDYLTTANHDTLAFAMIETRDAYDALDAI
LDVRGIDGVFVGPSDFSIAWSNGREANPHSDAIVEPISNIAHKAAAAGKIAGIYSPSPEFARRYISLGFRFLTISNDSGY
IRLAAETLVKAVRG

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=247, Percent_Identity=31.5789473684211, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788578, Length=239, Percent_Identity=31.7991631799163, Blast_Score=123, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27260; Mature: 27129

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSLSSRLRAGETVLSAWSSLPEPLTVEILAHSAFDAVTLDMQHGGHDEASILRSLGLV
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHH
LNAGKPPVVRIPVGRFDMASRALDFGAHAVIAPMINSVDDARRFAAAMKYPPVGERSWGV
EECCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
FRANADYGAPGSNDYLTTANHDTLAFAMIETRDAYDALDAILDVRGIDGVFVGPSDFSIA
EEECCCCCCCCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCEEE
WSNGREANPHSDAIVEPISNIAHKAAAAGKIAGIYSPSPEFARRYISLGFRFLTISNDSG
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCEEEEEEECCCC
YIRLAAETLVKAVRG
EEEEHHHHHHHHHCC
>Mature Secondary Structure 
SMSLSSRLRAGETVLSAWSSLPEPLTVEILAHSAFDAVTLDMQHGGHDEASILRSLGLV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHH
LNAGKPPVVRIPVGRFDMASRALDFGAHAVIAPMINSVDDARRFAAAMKYPPVGERSWGV
EECCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
FRANADYGAPGSNDYLTTANHDTLAFAMIETRDAYDALDAILDVRGIDGVFVGPSDFSIA
EEECCCCCCCCCCCEEEECCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCEEE
WSNGREANPHSDAIVEPISNIAHKAAAAGKIAGIYSPSPEFARRYISLGFRFLTISNDSG
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCEEEEEEECCCC
YIRLAAETLVKAVRG
EEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA