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The map label for this gene is moaE

Identifier: 225852216

GI number: 225852216

Start: 703573

End: 704064

Strand: Reverse

Name: moaE

Synonym: BMEA_A0732

Alternate gene names: 225852216

Gene position: 704064-703573 (Counterclockwise)

Preceding gene: 225852217

Following gene: 225852215

Centisome position: 33.12

GC content: 56.5

Gene sequence:

>492_bases
ATGGACGGGCAACCCACCTGTCCACTATCGATCAGCGTTCGCAGCGAAGATTTCGATATTGCCGCCGAGATCGACCGCAT
TGGCAAGAACCGCAGGGAAATCGGCGCGATTGTAACCTTTACCGGCCTTTGCCGCGATGAAGACGGACAGCTTGCCGCTC
TTGAGCTTGAGCATTATCCGGGCATGGCACAAGCCGAAATTTCGCGCATGGCAAAGCAGGCCCTGGAACGCTGGCCGCTG
AACGGCCTGACCATCATTCACCGCTATGGTCTGATTAAACCCGGCGAAAATATCGTTCTCGTCGTCGCCGCGTCCAGACA
TCGACACGCCGCTTTCGAGGCCGCTTCGTTTCTGATGGACTATATGAAAACCAATGCGCCTTTCTGGAAGCGTGAGCATC
TTGCCGATGGCACGGCTGGCGGCTGGGTGTCCTCCAAAGAAGAGGACGAAGAAAGCCGCAAGCGATGGGAAGAGCCTCGC
AAAAGCGAATGA

Upstream 100 bases:

>100_bases
GTTATTCGCGCCGCCATCAATCAGGAACACGCCGAACATGACGAACTGGTGAAGGATGGCGACGAAGTGGCGCTGTTCCC
TCCAATGACCGGGGGCTGAT

Downstream 100 bases:

>100_bases
AATGCCTGCAGCCTGGTTTGGACTGTGGTGATGAATTAACTATTTAACTCTCGAAAATACAGGAAAATTTGGGTAGCGCA
GAGTTGTACCGCCACAATTT

Product: molybdopterin biosynthesis MoaE protein

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MDGQPTCPLSISVRSEDFDIAAEIDRIGKNRREIGAIVTFTGLCRDEDGQLAALELEHYPGMAQAEISRMAKQALERWPL
NGLTIIHRYGLIKPGENIVLVVAASRHRHAAFEAASFLMDYMKTNAPFWKREHLADGTAGGWVSSKEEDEESRKRWEEPR
KSE

Sequences:

>Translated_163_residues
MDGQPTCPLSISVRSEDFDIAAEIDRIGKNRREIGAIVTFTGLCRDEDGQLAALELEHYPGMAQAEISRMAKQALERWPL
NGLTIIHRYGLIKPGENIVLVVAASRHRHAAFEAASFLMDYMKTNAPFWKREHLADGTAGGWVSSKEEDEESRKRWEEPR
KSE
>Mature_163_residues
MDGQPTCPLSISVRSEDFDIAAEIDRIGKNRREIGAIVTFTGLCRDEDGQLAALELEHYPGMAQAEISRMAKQALERWPL
NGLTIIHRYGLIKPGENIVLVVAASRHRHAAFEAASFLMDYMKTNAPFWKREHLADGTAGGWVSSKEEDEESRKRWEEPR
KSE

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family

Homologues:

Organism=Homo sapiens, GI4758732, Length=115, Percent_Identity=32.1739130434783, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1787003, Length=148, Percent_Identity=44.5945945945946, Blast_Score=129, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21356259, Length=116, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAE_BRUME (P65396)

Other databases:

- EMBL:   AE008917
- PIR:   AH3408
- RefSeq:   NP_540171.1
- ProteinModelPortal:   P65396
- SMR:   P65396
- GeneID:   1196965
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI1254
- HOGENOM:   HBG460564
- OMA:   RERWPLN
- PhylomeDB:   P65396
- ProtClustDB:   CLSK898364
- BioCyc:   BMEL224914:BMEI1254-MONOMER
- InterPro:   IPR003448
- Gene3D:   G3DSA:3.90.1170.40
- PANTHER:   PTHR23404:SF2

Pfam domain/function: PF02391 MoaE; SSF54690 Mb_biosynth_MoaE

EC number: NA

Molecular weight: Translated: 18396; Mature: 18396

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGQPTCPLSISVRSEDFDIAAEIDRIGKNRREIGAIVTFTGLCRDEDGQLAALELEHYP
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHEEEEEECEECCCCCEEEEEECCCC
GMAQAEISRMAKQALERWPLNGLTIIHRYGLIKPGENIVLVVAASRHRHAAFEAASFLMD
CHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
YMKTNAPFWKREHLADGTAGGWVSSKEEDEESRKRWEEPRKSE
HHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDGQPTCPLSISVRSEDFDIAAEIDRIGKNRREIGAIVTFTGLCRDEDGQLAALELEHYP
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHEEEEEECEECCCCCEEEEEECCCC
GMAQAEISRMAKQALERWPLNGLTIIHRYGLIKPGENIVLVVAASRHRHAAFEAASFLMD
CHHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
YMKTNAPFWKREHLADGTAGGWVSSKEEDEESRKRWEEPRKSE
HHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688