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The map label for this gene is gmd [H]

Identifier: 225852058

GI number: 225852058

Start: 541015

End: 542103

Strand: Reverse

Name: gmd [H]

Synonym: BMEA_A0560

Alternate gene names: 225852058

Gene position: 542103-541015 (Counterclockwise)

Preceding gene: 225852066

Following gene: 225852057

Centisome position: 25.5

GC content: 47.84

Gene sequence:

>1089_bases
GTGAAGAAAGTCGCACTTATCACAGGTATCACCGGCCAAGACGGCGCCTACCTTGCGGAATTGCTTCTACAAAAAGGTTA
TGCAGTACACGGGATTAAGCGTCGCACCTCGCAATTTAACACAGCACGTATCGATCATCTGTACCATGATCCGCACGAGC
AGGGTGTCGATCTAACACTTCATCATGGCGATCTAACAGATACGTCAAGCTTGGTTCGAATCATGCAGCTCGTTCGGCCG
GATGAAGTTTATAACCTTGGCGCACAAAGCCATGTTGCTGTTTCATTTGAGGAGCCGGAGTATACTGCGAACTCAGACGC
ACTAGGTGCCCTTCGTCTGCTAGAGGCGATCCGAATTCTTGGAATGGAGAAGCAGACCCGGTACTACCAAGCATCCACCT
CTGAGCTTTATGGTCTGGTTCAAGAAATCCCGCAAAAAGAAACGACGCCTTTTTACCCTCGGTCGCCCTATGCCGCAGCG
AAGCTTTATGCCTATTGGATTACGGTAAACTACCGAGAAGCGTATGGTATATATGCTTGTAATGGCATACTTTTTAACCA
TGAATCTCCACTTAGAGGAGAGACTTTTGTCACGAGAAAAATTACTCGCGCTTTAGCTAGAATTAAGATCGGTAATCAGA
ATCGCTTATACTTAGGGAATTTGGACTCATTACGCGACTGGGGGCATGCTCGGGACTACGTCGAGATGCAGTGGTTAATG
CTTCAACAGGATCAACCAGAAGATTTTGTAATAGCAACTGGTAAACAATATTCAGTTCGAGAATTTGTCACGCTCGCTGC
TAAAGACATAGGCATTGAGATGCGATGGGAAGGTAGCGGTGAAGCCGAGAAGGGTTATGATGCCAAGACCGGTGCATGTA
TTGTCGAGGTCGATCCTAGATACTTTCGACCAGCCGAGGTGGAAACTCTTCTGGGAGATGCGACCAAGGCGCATCAAAAA
CTGGGCTGGAAGCCGAAAATCTCTTTTGAGACGCTTGTATCTGAGATGATAGCAGAAGACTTAGCGGCGGCGGCTCGTGA
GAATTTGCTGCGCGAACATGGTCACCAATTTTTCGCCCCTAAGGAATGA

Upstream 100 bases:

>100_bases
TCGCGTTCAAATACGTGTAATGCTCCACGAGCAAGTTGAGATTCATAGGAAGTTGGCCATGCGTTTACAGTTGGCATGGC
AAAAACGGGAATGAACAATC

Downstream 100 bases:

>100_bases
TCCATACATGGATATACCAGTTTACTCTCCCTATCTCTGTGGGAATTTCAAAAAGTATGTGAACGAATGCCTTGATACAG
GGTGGATCTCGTCGAGGGGT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKKVALITGITGQDGAYLAELLLQKGYAVHGIKRRTSQFNTARIDHLYHDPHEQGVDLTLHHGDLTDTSSLVRIMQLVRP
DEVYNLGAQSHVAVSFEEPEYTANSDALGALRLLEAIRILGMEKQTRYYQASTSELYGLVQEIPQKETTPFYPRSPYAAA
KLYAYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRALARIKIGNQNRLYLGNLDSLRDWGHARDYVEMQWLM
LQQDQPEDFVIATGKQYSVREFVTLAAKDIGIEMRWEGSGEAEKGYDAKTGACIVEVDPRYFRPAEVETLLGDATKAHQK
LGWKPKISFETLVSEMIAEDLAAAARENLLREHGHQFFAPKE

Sequences:

>Translated_362_residues
MKKVALITGITGQDGAYLAELLLQKGYAVHGIKRRTSQFNTARIDHLYHDPHEQGVDLTLHHGDLTDTSSLVRIMQLVRP
DEVYNLGAQSHVAVSFEEPEYTANSDALGALRLLEAIRILGMEKQTRYYQASTSELYGLVQEIPQKETTPFYPRSPYAAA
KLYAYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRALARIKIGNQNRLYLGNLDSLRDWGHARDYVEMQWLM
LQQDQPEDFVIATGKQYSVREFVTLAAKDIGIEMRWEGSGEAEKGYDAKTGACIVEVDPRYFRPAEVETLLGDATKAHQK
LGWKPKISFETLVSEMIAEDLAAAARENLLREHGHQFFAPKE
>Mature_362_residues
MKKVALITGITGQDGAYLAELLLQKGYAVHGIKRRTSQFNTARIDHLYHDPHEQGVDLTLHHGDLTDTSSLVRIMQLVRP
DEVYNLGAQSHVAVSFEEPEYTANSDALGALRLLEAIRILGMEKQTRYYQASTSELYGLVQEIPQKETTPFYPRSPYAAA
KLYAYWITVNYREAYGIYACNGILFNHESPLRGETFVTRKITRALARIKIGNQNRLYLGNLDSLRDWGHARDYVEMQWLM
LQQDQPEDFVIATGKQYSVREFVTLAAKDIGIEMRWEGSGEAEKGYDAKTGACIVEVDPRYFRPAEVETLLGDATKAHQK
LGWKPKISFETLVSEMIAEDLAAAARENLLREHGHQFFAPKE

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=62.280701754386, Blast_Score=450, Evalue=1e-126,
Organism=Escherichia coli, GI1788366, Length=365, Percent_Identity=72.3287671232877, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=25.8720930232558, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=295, Percent_Identity=25.7627118644068, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=63.1578947368421, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17507723, Length=343, Percent_Identity=62.9737609329446, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=63.1578947368421, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=63.1578947368421, Blast_Score=463, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=63.1578947368421, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=63.1578947368421, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24158427, Length=340, Percent_Identity=62.3529411764706, Blast_Score=452, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 41063; Mature: 41063

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVALITGITGQDGAYLAELLLQKGYAVHGIKRRTSQFNTARIDHLYHDPHEQGVDLTL
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCCCCCCEEEE
HHGDLTDTSSLVRIMQLVRPDEVYNLGAQSHVAVSFEEPEYTANSDALGALRLLEAIRIL
ECCCCCCHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
GMEKQTRYYQASTSELYGLVQEIPQKETTPFYPRSPYAAAKLYAYWITVNYREAYGIYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEEEEEEEHHCCCEEEE
NGILFNHESPLRGETFVTRKITRALARIKIGNQNRLYLGNLDSLRDWGHARDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHHHHHHHEEE
LQQDQPEDFVIATGKQYSVREFVTLAAKDIGIEMRWEGSGEAEKGYDAKTGACIVEVDPR
ECCCCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCC
YFRPAEVETLLGDATKAHQKLGWKPKISFETLVSEMIAEDLAAAARENLLREHGHQFFAP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KE
CC
>Mature Secondary Structure
MKKVALITGITGQDGAYLAELLLQKGYAVHGIKRRTSQFNTARIDHLYHDPHEQGVDLTL
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCCCCCCEEEE
HHGDLTDTSSLVRIMQLVRPDEVYNLGAQSHVAVSFEEPEYTANSDALGALRLLEAIRIL
ECCCCCCHHHHHHHHHHHCCCHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
GMEKQTRYYQASTSELYGLVQEIPQKETTPFYPRSPYAAAKLYAYWITVNYREAYGIYAC
CCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEEEEEEEHHCCCEEEE
NGILFNHESPLRGETFVTRKITRALARIKIGNQNRLYLGNLDSLRDWGHARDYVEMQWLM
CEEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHHHHHHHEEE
LQQDQPEDFVIATGKQYSVREFVTLAAKDIGIEMRWEGSGEAEKGYDAKTGACIVEVDPR
ECCCCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCC
YFRPAEVETLLGDATKAHQKLGWKPKISFETLVSEMIAEDLAAAARENLLREHGHQFFAP
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]