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The map label for this gene is metZ [H]

Identifier: 225851851

GI number: 225851851

Start: 337739

End: 338935

Strand: Reverse

Name: metZ [H]

Synonym: BMEA_A0340

Alternate gene names: 225851851

Gene position: 338935-337739 (Counterclockwise)

Preceding gene: 225851857

Following gene: 225851850

Centisome position: 15.94

GC content: 59.31

Gene sequence:

>1197_bases
ATGACGACTGAGAATGCCCGCAAATTTCGCCCTGCAACCGAACTCGTCCATGCCGGATCGTTGCGCTCCGGTTTCGCCGA
AATGTCGGAAGCCCTGTTTTTGACGCAAGGCTTCCTTTACCCCACGGCGGAAGCTGCCGAAGCGCGTTTCAAGGGTGAAG
ATCCGGGTTTCATCTATTCGCGCTACGCCAACCCGACCACCGACATGTTTGAAAAGCGCATGTGTGCGCTGGAAGGCGCG
GAAGACGCCCGCGCCACCGCCTCCGGCATGGCCGCCGTTGCCGCCGCCATTCTCTGCCAGGTACAGGCGGGCGATCATGT
CATCTCGGCCCGCGCCGTCTTCGGCTCGTGTCGTTATATCGTCGAAACCGTCCTGCCGCGCTATGGCGTGGAAGTCTCGA
TCATCGACGGCTCGGATGTGGCCAACTGGAAAGCGGCCGTGCGCCCCAATACCAAGGTCATGTTTCTTGAAAGCCCGTCG
AACCCGACACTCGAAATCATCGACATTGCCGCCGTTGCACAGATTGCCAACGAAGCGGGCGCAAAGCTGATCGTGGACAA
TGTTTTCGCCACGCCGCTTTTCCAGAAGCCGCTGGAGCTTGGCGCACATATCGTCGTCTATTCAGCCACGAAACATATTG
ACGGCCAGGGCCGCTGCCTCGGCGGCGTGGTGCTTTCCGACCGCGAATGGATCGAAACCACATTGCAGGATTATTTCCGC
CATACGGGACCGGGCCTCAGCCCCTTCAATGCATGGATCATGCTGAAGGGCCTGGAAACGCTTTCGGTGCGCGTGCGCCA
GCAGACGCAATCGGCTGCCGCAATCGCCGACTTCCTTGCAGGCAAGCCGGGCGTCAAGCGTGTGATCTATCCGGGCCGCG
CCGACCATCCGCAGACCGACATCATTGCTAAGCAGATGACAGGCGGATCCACGCTCATCGCGCTGGAGCTGGAGGGCGGC
AAGGAAGCAGCTTTCAAGTTTGAAAATGCATTGCAAATTTTCAGCATCACCAACAATCTGGGCGACACCAAGAGCCTGAT
TACCCATCCGGCCACCACGACGCACAAGAACCTCTCCAACGAGGCGAAGGCAGAACTCGGCATTTCGGATGGCCTGCTGC
GCGTTTCGGTCGGCCTCGAAGACACCGACGATCTTCTGGAAGATGTTGAAGTGGCGCTGAAAGTAGCGCGCGGCTGA

Upstream 100 bases:

>100_bases
TTCGTTACCGGCCTTTTGCGTTTTCGCAAGGCCGGTTTTTTATTGGCCGCCGAGGCTTTCTTCCTGCCCCGGCCGACCAG
CTTTCGACAGGTTGGAAAAA

Downstream 100 bases:

>100_bases
AAACCGGCCAAATCTGCTTTTTTTGCCGCAAAAACAGCTAAAATACCCCCGATTTCCGGAATAAACCGGTAATCGGGGCG
AATTTATGCCGCTCCATCGT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTTENARKFRPATELVHAGSLRSGFAEMSEALFLTQGFLYPTAEAAEARFKGEDPGFIYSRYANPTTDMFEKRMCALEGA
EDARATASGMAAVAAAILCQVQAGDHVISARAVFGSCRYIVETVLPRYGVEVSIIDGSDVANWKAAVRPNTKVMFLESPS
NPTLEIIDIAAVAQIANEAGAKLIVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLSDREWIETTLQDYFR
HTGPGLSPFNAWIMLKGLETLSVRVRQQTQSAAAIADFLAGKPGVKRVIYPGRADHPQTDIIAKQMTGGSTLIALELEGG
KEAAFKFENALQIFSITNNLGDTKSLITHPATTTHKNLSNEAKAELGISDGLLRVSVGLEDTDDLLEDVEVALKVARG

Sequences:

>Translated_398_residues
MTTENARKFRPATELVHAGSLRSGFAEMSEALFLTQGFLYPTAEAAEARFKGEDPGFIYSRYANPTTDMFEKRMCALEGA
EDARATASGMAAVAAAILCQVQAGDHVISARAVFGSCRYIVETVLPRYGVEVSIIDGSDVANWKAAVRPNTKVMFLESPS
NPTLEIIDIAAVAQIANEAGAKLIVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLSDREWIETTLQDYFR
HTGPGLSPFNAWIMLKGLETLSVRVRQQTQSAAAIADFLAGKPGVKRVIYPGRADHPQTDIIAKQMTGGSTLIALELEGG
KEAAFKFENALQIFSITNNLGDTKSLITHPATTTHKNLSNEAKAELGISDGLLRVSVGLEDTDDLLEDVEVALKVARG
>Mature_397_residues
TTENARKFRPATELVHAGSLRSGFAEMSEALFLTQGFLYPTAEAAEARFKGEDPGFIYSRYANPTTDMFEKRMCALEGAE
DARATASGMAAVAAAILCQVQAGDHVISARAVFGSCRYIVETVLPRYGVEVSIIDGSDVANWKAAVRPNTKVMFLESPSN
PTLEIIDIAAVAQIANEAGAKLIVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLSDREWIETTLQDYFRH
TGPGLSPFNAWIMLKGLETLSVRVRQQTQSAAAIADFLAGKPGVKRVIYPGRADHPQTDIIAKQMTGGSTLIALELEGGK
EAAFKFENALQIFSITNNLGDTKSLITHPATTTHKNLSNEAKAELGISDGLLRVSVGLEDTDDLLEDVEVALKVARG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=347, Percent_Identity=38.6167146974063, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI299473759, Length=347, Percent_Identity=35.4466858789625, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI25453487, Length=354, Percent_Identity=32.7683615819209, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1790375, Length=338, Percent_Identity=33.7278106508876, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1789383, Length=389, Percent_Identity=28.5347043701799, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17560056, Length=354, Percent_Identity=39.8305084745763, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17538055, Length=350, Percent_Identity=39.4285714285714, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=32.7913279132791, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=404, Percent_Identity=37.6237623762376, Blast_Score=238, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6323333, Length=427, Percent_Identity=28.5714285714286, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=269, Percent_Identity=29.7397769516729, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=25.8536585365854, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=395, Percent_Identity=28.1012658227848, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922554, Length=347, Percent_Identity=35.4466858789625, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42828; Mature: 42697

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTENARKFRPATELVHAGSLRSGFAEMSEALFLTQGFLYPTAEAAEARFKGEDPGFIYS
CCCCCCHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEE
RYANPTTDMFEKRMCALEGAEDARATASGMAAVAAAILCQVQAGDHVISARAVFGSCRYI
ECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
VETVLPRYGVEVSIIDGSDVANWKAAVRPNTKVMFLESPSNPTLEIIDIAAVAQIANEAG
HHHHHHHCCCEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCEEEEHHHHHHHHHHHCC
AKLIVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLSDREWIETTLQDYFR
CEEEEHHHHHCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
HTGPGLSPFNAWIMLKGLETLSVRVRQQTQSAAAIADFLAGKPGVKRVIYPGRADHPQTD
HCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHH
IIAKQMTGGSTLIALELEGGKEAAFKFENALQIFSITNNLGDTKSLITHPATTTHKNLSN
HHHHHCCCCCEEEEEEECCCCHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCC
EAKAELGISDGLLRVSVGLEDTDDLLEDVEVALKVARG
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTENARKFRPATELVHAGSLRSGFAEMSEALFLTQGFLYPTAEAAEARFKGEDPGFIYS
CCCCCHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEE
RYANPTTDMFEKRMCALEGAEDARATASGMAAVAAAILCQVQAGDHVISARAVFGSCRYI
ECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
VETVLPRYGVEVSIIDGSDVANWKAAVRPNTKVMFLESPSNPTLEIIDIAAVAQIANEAG
HHHHHHHCCCEEEEECCCCCCCCEEEECCCCEEEEEECCCCCCEEEEHHHHHHHHHHHCC
AKLIVDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLSDREWIETTLQDYFR
CEEEEHHHHHCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
HTGPGLSPFNAWIMLKGLETLSVRVRQQTQSAAAIADFLAGKPGVKRVIYPGRADHPQTD
HCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHH
IIAKQMTGGSTLIALELEGGKEAAFKFENALQIFSITNNLGDTKSLITHPATTTHKNLSN
HHHHHCCCCCEEEEEEECCCCHHHHHHHCCEEEEEECCCCCCCHHHHCCCCCCCCCCCCC
EAKAELGISDGLLRVSVGLEDTDDLLEDVEVALKVARG
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]