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The map label for this gene is artJ [H]

Identifier: 225851842

GI number: 225851842

Start: 327147

End: 327989

Strand: Reverse

Name: artJ [H]

Synonym: BMEA_A0331

Alternate gene names: 225851842

Gene position: 327989-327147 (Counterclockwise)

Preceding gene: 225851844

Following gene: 225851841

Centisome position: 15.43

GC content: 58.24

Gene sequence:

>843_bases
ATGAAATCCTGTCTGTTGAGCCTGACGATAAGCATGTGCCTTTTCGCTCCTGCCGCCCATGCGGCGGGGGAAGAACCGGC
TGCACCGCATTTCTTCAACCAGAAAGAGCGCCTGCCCCTGCCTTCGCTGCAAGGGCTCAATCGTCTGCGCTTCATCACCA
CCGTCGATTTTCCGCCATTCAACAGGCGTAACGAACGCGGGCAGCTTTCCGGCTACAACATTGATCTGGCCAAGGCGCTT
TGCCAGCAGCTCAAGATCGAGGACATCTGCCAGATCGAGGCGGTGCCGTGGGCGGAACTGGAAGAGCGTGTGCTGGATGG
ACAGGCGGACGCGATCATCGCGGGGTGGGATCCGACCGACAGGAACCGGGAAAAATTCACCTTCACCCGCAGCTATATGC
GCCTGCCCGCACGCTTTGCGACCTCCAATGCCAAAAGCTTTACAGAACCCGCTGCAAAGGCGACGCAGGACAAGCCCGTC
GGCGTGATTGCCGGGACGGCGCATGAAGCGCTTTTGAAGAACTATTTCCCACAGGCAAAACCCGTGCCCTACCCCAATCG
CGATGCCATGCTGACCGCATTGAAGGACGGCAAGATCGACACGGTTTTCGATGACGGCATGTCCCTCTCGGCCTGGCTCG
ACAGCGCAGAAGGCAGCGCCTGTTGTACTTTCACGGACGGGCCCTATCTGGCGCCTCAATATCTGGGTTCCGGCCTCAGC
ATCGCTGTTACCCGGCAAAATTCCGCCCTGGCAAGCGCCTTCAACAATGCCCTGCAAGCCCTGCAACAAGAAGGTAAGCT
GACCGAGCTTTACCTGAGATATTTCCCGGCAAGCTTTTATTAG

Upstream 100 bases:

>100_bases
ATATACGAATTATCAGGATGCCCGTTACTGCTATCGCCGATGGCGCTTGCATAGGCGCCGTTAACCCGGAATTATGGGGA
ATATTATAGGGTCGCCGGCG

Downstream 100 bases:

>100_bases
AGCATTTCCAGCCCCGCCTCGCACCCGCAAAGCACATAGCGGCGTCCACGGCCCGCACATGTGCGATTTTGACGCACTTT
AGCGAAATTCCGGCTGAAAC

Product: extracellular solute-binding protein family 3

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPFNRRNERGQLSGYNIDLAKAL
CQQLKIEDICQIEAVPWAELEERVLDGQADAIIAGWDPTDRNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPV
GVIAGTAHEALLKNYFPQAKPVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY

Sequences:

>Translated_280_residues
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPFNRRNERGQLSGYNIDLAKAL
CQQLKIEDICQIEAVPWAELEERVLDGQADAIIAGWDPTDRNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPV
GVIAGTAHEALLKNYFPQAKPVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY
>Mature_280_residues
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPFNRRNERGQLSGYNIDLAKAL
CQQLKIEDICQIEAVPWAELEERVLDGQADAIIAGWDPTDRNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPV
GVIAGTAHEALLKNYFPQAKPVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=231, Percent_Identity=31.6017316017316, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787088, Length=235, Percent_Identity=28.936170212766, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=23.5042735042735, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=25.531914893617, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=22.8699551569507, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30896; Mature: 30896

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCC
NRRNERGQLSGYNIDLAKALCQQLKIEDICQIEAVPWAELEERVLDGQADAIIAGWDPTD
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCC
RNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPVGVIAGTAHEALLKNYFPQAK
CCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
PVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
CCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCEECHHHHCCCCE
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY
EEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCC
NRRNERGQLSGYNIDLAKALCQQLKIEDICQIEAVPWAELEERVLDGQADAIIAGWDPTD
CCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCC
RNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPVGVIAGTAHEALLKNYFPQAK
CCCCHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
PVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
CCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCEECHHHHCCCCE
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY
EEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]