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The map label for this gene is ogt [H]

Identifier: 225851717

GI number: 225851717

Start: 195266

End: 195844

Strand: Reverse

Name: ogt [H]

Synonym: BMEA_A0192

Alternate gene names: 225851717

Gene position: 195844-195266 (Counterclockwise)

Preceding gene: 225851719

Following gene: 225851705

Centisome position: 9.21

GC content: 59.24

Gene sequence:

>579_bases
ATGGAATCGTTTGGCATCACCGTTTTTGAAACGCCCATCGGGCCCTGCGGCATTGCATGGCGCGGGAGCAAAATCGTGGG
CGTGGAGATCGGCGAGGCGGACGAGAAAGAAACGCGCCATCGACTTCGTGAGCGTTTTGCGGGCGGGGAATATGCTGAAC
CGCCCGCTTTCATCAGGCAAACTATTGAAAAAGTGCGCGCATTGCTTGATGGCGCATCGCCGGATTTTTCCGATACACCG
CTGGCGCTTGATTCGGTCCCGGACCTCAACCGGCGCGTTTATGAAATCATTCTGGAACTGAAACCGGGTGAAACCACCAC
TTATGGCGCGATTGCGCGGCGCCTTGGCGATGTGAGCCTGTCGCAGGCAGTGGGCTATGCGCTCGGCAAGAATCCGTTTC
CCATCATCGTGCCCTGCCACCGCGTTCTGGGATCAAACGGCAAGGTGGGCGGCTTTTCGGCTGCGGGCGGCACGGCCACC
AAGCTGCGCCTTCTCAACATAGAGCGCGCCAGAACGACAAGCGAGCCAGACCTTTTCGGCGGACTGCCCTTACAGGAACG
GCCACAGACAGGCTGGTAA

Upstream 100 bases:

>100_bases
CTGCAGCATTTTGCGCAAATACTACGACTTTTCACCTCTTAACCGGTTCATCCGGGTTCCATCTGCCCCTGTATGTTTCA
AATCGTGCTATTATTCGGTC

Downstream 100 bases:

>100_bases
TCAGTTCTTGTCTGTCACCACATCGGCGGAAAAGCGCAGTTCGCGCATCGGGCGCACAAGCTGCGTCGTGGCATCATAAG
TCGGATGGTTCTTGAAAGCG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MESFGITVFETPIGPCGIAWRGSKIVGVEIGEADEKETRHRLRERFAGGEYAEPPAFIRQTIEKVRALLDGASPDFSDTP
LALDSVPDLNRRVYEIILELKPGETTTYGAIARRLGDVSLSQAVGYALGKNPFPIIVPCHRVLGSNGKVGGFSAAGGTAT
KLRLLNIERARTTSEPDLFGGLPLQERPQTGW

Sequences:

>Translated_192_residues
MESFGITVFETPIGPCGIAWRGSKIVGVEIGEADEKETRHRLRERFAGGEYAEPPAFIRQTIEKVRALLDGASPDFSDTP
LALDSVPDLNRRVYEIILELKPGETTTYGAIARRLGDVSLSQAVGYALGKNPFPIIVPCHRVLGSNGKVGGFSAAGGTAT
KLRLLNIERARTTSEPDLFGGLPLQERPQTGW
>Mature_192_residues
MESFGITVFETPIGPCGIAWRGSKIVGVEIGEADEKETRHRLRERFAGGEYAEPPAFIRQTIEKVRALLDGASPDFSDTP
LALDSVPDLNRRVYEIILELKPGETTTYGAIARRLGDVSLSQAVGYALGKNPFPIIVPCHRVLGSNGKVGGFSAAGGTAT
KLRLLNIERARTTSEPDLFGGLPLQERPQTGW

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=36.7924528301887, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=79, Percent_Identity=44.3037974683544, Blast_Score=64, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115533070, Length=79, Percent_Identity=44.3037974683544, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320001, Length=117, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20777; Mature: 20777

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESFGITVFETPIGPCGIAWRGSKIVGVEIGEADEKETRHRLRERFAGGEYAEPPAFIRQ
CCCCCCEEEECCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
TIEKVRALLDGASPDFSDTPLALDSVPDLNRRVYEIILELKPGETTTYGAIARRLGDVSL
HHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHH
SQAVGYALGKNPFPIIVPCHRVLGSNGKVGGFSAAGGTATKLRLLNIERARTTSEPDLFG
HHHHHHHHCCCCCEEEEECHHHHCCCCCCCCEECCCCCEEEEEEEECHHHCCCCCCCCCC
GLPLQERPQTGW
CCCCCCCCCCCC
>Mature Secondary Structure
MESFGITVFETPIGPCGIAWRGSKIVGVEIGEADEKETRHRLRERFAGGEYAEPPAFIRQ
CCCCCCEEEECCCCCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
TIEKVRALLDGASPDFSDTPLALDSVPDLNRRVYEIILELKPGETTTYGAIARRLGDVSL
HHHHHHHHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHH
SQAVGYALGKNPFPIIVPCHRVLGSNGKVGGFSAAGGTATKLRLLNIERARTTSEPDLFG
HHHHHHHHCCCCCEEEEECHHHHCCCCCCCCEECCCCCEEEEEEEECHHHCCCCCCCCCC
GLPLQERPQTGW
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]