Click here to switch to the map view.

The map label for this gene is grpE

Identifier: 225851704

GI number: 225851704

Start: 183377

End: 184069

Strand: Reverse

Name: grpE

Synonym: BMEA_A0177

Alternate gene names: 225851704

Gene position: 184069-183377 (Counterclockwise)

Preceding gene: 225851705

Following gene: 225851703

Centisome position: 8.66

GC content: 59.45

Gene sequence:

>693_bases
ATGGCTGACGAAAAGAACAAACCCGAAAACCCCGATCTTGACCAGCGCGATATCAACAATCCGCGGGACCGTGAGGCGCT
GAAGCAGGCTGCCGACGATTTCCTGAAAGCGCGCCGGGCGGAAGCCAGGGCAGAAGCCGCCGCAGACGAGGCCGAAGGGG
AAGTGGACGAAACCGCAAACCGTATCGCGGTATTGGAAGCGGACAATACGGAACTGAAAGACCAGATGCTTCGCGTTGCC
GCTGAAATGGAAAATCTGCGCAAGCGCACCCAGCGCGACGTGCAGGATGCGCGCGCTTACGCCATCACCAATTTTGCTCG
TGACATGCTTTCGGTTTCGGACAATCTGCGTCGCGCGCTCGATGCCATTCCGGCCGATGCGCTGGAAGCCGATTCCAACC
TGAAATCGCTTTCCGAAGGCGTGGAAATGACCGAACGCGCCATGCTGCTGGCACTGGAACGCCATGGCGTGAAGAAGCTG
GAACCGGAAGGCCAGAAATTCGACCCGAATTTCCATCAGGCCATGTTTGAAGTGCCCAATCCAGATCTGCCCAACAATAC
GGTGGTGCAGGTGGTGCAGGCCGGTTACGCCATCGGCGACCGCGTGCTGCGCCCGGCCATGGTGGGCGTTTCCAAGGGAG
GCCCGAAGGTTTCCGCCGAAAACGGCGCCTCGACCTCAGAAGACAATGCGTGA

Upstream 100 bases:

>100_bases
AGATTTGCCGGTGCGCACCAGCCCCCAGAAATTAGACCTTGATTTTAAAGGCACGAAACTCGATATCCGCCCCTAACGAT
CTTCAAGACGGAAGCGCACT

Downstream 100 bases:

>100_bases
TCCGATTGCGCAGGCGTATTGAAAAAGCAGCGGCATCATTTGCCGCTCTTTTTATTTCCGCTTTTTCTATTACGCTTGAG
GCAGCGCGGGTGGAAACTTG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MADEKNKPENPDLDQRDINNPRDREALKQAADDFLKARRAEARAEAAADEAEGEVDETANRIAVLEADNTELKDQMLRVA
AEMENLRKRTQRDVQDARAYAITNFARDMLSVSDNLRRALDAIPADALEADSNLKSLSEGVEMTERAMLLALERHGVKKL
EPEGQKFDPNFHQAMFEVPNPDLPNNTVVQVVQAGYAIGDRVLRPAMVGVSKGGPKVSAENGASTSEDNA

Sequences:

>Translated_230_residues
MADEKNKPENPDLDQRDINNPRDREALKQAADDFLKARRAEARAEAAADEAEGEVDETANRIAVLEADNTELKDQMLRVA
AEMENLRKRTQRDVQDARAYAITNFARDMLSVSDNLRRALDAIPADALEADSNLKSLSEGVEMTERAMLLALERHGVKKL
EPEGQKFDPNFHQAMFEVPNPDLPNNTVVQVVQAGYAIGDRVLRPAMVGVSKGGPKVSAENGASTSEDNA
>Mature_229_residues
ADEKNKPENPDLDQRDINNPRDREALKQAADDFLKARRAEARAEAAADEAEGEVDETANRIAVLEADNTELKDQMLRVAA
EMENLRKRTQRDVQDARAYAITNFARDMLSVSDNLRRALDAIPADALEADSNLKSLSEGVEMTERAMLLALERHGVKKLE
PEGQKFDPNFHQAMFEVPNPDLPNNTVVQVVQAGYAIGDRVLRPAMVGVSKGGPKVSAENGASTSEDNA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=149, Percent_Identity=40.2684563758389, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI40255109, Length=176, Percent_Identity=31.8181818181818, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788967, Length=188, Percent_Identity=34.5744680851064, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=149, Percent_Identity=38.255033557047, Blast_Score=113, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324806, Length=164, Percent_Identity=39.0243902439024, Blast_Score=136, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=39.4285714285714, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_BRUC2 (A9M7B6)

Other databases:

- EMBL:   CP000872
- RefSeq:   YP_001592046.1
- ProteinModelPortal:   A9M7B6
- SMR:   A9M7B6
- GeneID:   5784581
- GenomeReviews:   CP000872_GR
- KEGG:   bcs:BCAN_A0176
- HOGENOM:   HBG732630
- OMA:   IENVMEN
- ProtClustDB:   PRK14141
- BioCyc:   BCAN483179:BCAN_A0176-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 25310; Mature: 25179

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEKNKPENPDLDQRDINNPRDREALKQAADDFLKARRAEARAEAAADEAEGEVDETAN
CCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHC
RIAVLEADNTELKDQMLRVAAEMENLRKRTQRDVQDARAYAITNFARDMLSVSDNLRRAL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAIPADALEADSNLKSLSEGVEMTERAMLLALERHGVKKLEPEGQKFDPNFHQAMFEVPN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCC
PDLPNNTVVQVVQAGYAIGDRVLRPAMVGVSKGGPKVSAENGASTSEDNA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADEKNKPENPDLDQRDINNPRDREALKQAADDFLKARRAEARAEAAADEAEGEVDETAN
CCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHC
RIAVLEADNTELKDQMLRVAAEMENLRKRTQRDVQDARAYAITNFARDMLSVSDNLRRAL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAIPADALEADSNLKSLSEGVEMTERAMLLALERHGVKKLEPEGQKFDPNFHQAMFEVPN
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHCCC
PDLPNNTVVQVVQAGYAIGDRVLRPAMVGVSKGGPKVSAENGASTSEDNA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA