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The map label for this gene is ntcA [H]

Identifier: 225686127

GI number: 225686127

Start: 264577

End: 265275

Strand: Direct

Name: ntcA [H]

Synonym: BMEA_B0282

Alternate gene names: 225686127

Gene position: 264577-265275 (Clockwise)

Preceding gene: 225686126

Following gene: 225686128

Centisome position: 22.32

GC content: 61.23

Gene sequence:

>699_bases
ATGGCGGCAATAATCGACTGGGCTCTCGTTAGAAGCCTGCCCGTCTTTTCGGGAATGGATGATGCTGCCTTTGAACAGCT
CATGGGGCTGGCCATGCCGCGGGCGGTTCCCAAGGGAAGCGCCGTTTTCAAACAGGGCGAAGAGGCCAGGCTTTTCTATC
TTCTGGTGCAGGGGCGGCTGAAGGTCATGCAGGTGACGGCGGACGGCCAGCAGCTTATTGTGCGTGTGGTCAACCCCGGC
GAATTTTTCGGCTTCGCCATGGCGCTGGGGCGCGAGGACTATCCCGGTACGCCGCTTGCGGTGGTGGATTGCAGCGTTAT
TGCCTGGCCGATGGAAATGATGACGGCTTTCATGGCAAGCAATCCTGCGCTTGCCGTCAATACCATGCAGATGATCGGGC
GCAGGCTGGATGCGGCGCATAGCCGGATACGCGAGATTTCGACGCAGGAAGTGGAGCAGCGCGTGGCCCATGCGGTGCTG
CGGCTGGTGCGCGAGGCGGGCATCCAGGAAGATACGGGACTGCGCATCGATTTCCCGGTTTCACGGCAGGATATCGCCGA
ACTGACGGGGACGACACTGCACACGGTTTCACGCATCGTCAGCCAATGGCAGAGCAAGGGCTGGGTGGAGGGCGGCAGGC
AATCCCTGCTCGTGCGCGACCTTCGCCGCTTGCGGCAAATTGCCGAAGGCGAAATCTGA

Upstream 100 bases:

>100_bases
TTTCTTTTTGAGGATCGGTCTGATAGCGAACCCTTTGCACCCCCCATGCACCAGTGACGGCGCTCCCCGCGCTGGCGCTT
GGGGTATTGGAGAGGGAAAC

Downstream 100 bases:

>100_bases
CTATCTTCCTCAACTACATGAATCCTGTTTGATTTTAATCAAAGATAGCCGTCATCGGGGGGGGGATAAGCCTTTGTTTG
CGCGGCTCATCCGGCACGAT

Product: transcriptional regulator Crp/Fnr family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MAAIIDWALVRSLPVFSGMDDAAFEQLMGLAMPRAVPKGSAVFKQGEEARLFYLLVQGRLKVMQVTADGQQLIVRVVNPG
EFFGFAMALGREDYPGTPLAVVDCSVIAWPMEMMTAFMASNPALAVNTMQMIGRRLDAAHSRIREISTQEVEQRVAHAVL
RLVREAGIQEDTGLRIDFPVSRQDIAELTGTTLHTVSRIVSQWQSKGWVEGGRQSLLVRDLRRLRQIAEGEI

Sequences:

>Translated_232_residues
MAAIIDWALVRSLPVFSGMDDAAFEQLMGLAMPRAVPKGSAVFKQGEEARLFYLLVQGRLKVMQVTADGQQLIVRVVNPG
EFFGFAMALGREDYPGTPLAVVDCSVIAWPMEMMTAFMASNPALAVNTMQMIGRRLDAAHSRIREISTQEVEQRVAHAVL
RLVREAGIQEDTGLRIDFPVSRQDIAELTGTTLHTVSRIVSQWQSKGWVEGGRQSLLVRDLRRLRQIAEGEI
>Mature_231_residues
AAIIDWALVRSLPVFSGMDDAAFEQLMGLAMPRAVPKGSAVFKQGEEARLFYLLVQGRLKVMQVTADGQQLIVRVVNPGE
FFGFAMALGREDYPGTPLAVVDCSVIAWPMEMMTAFMASNPALAVNTMQMIGRRLDAAHSRIREISTQEVEQRVAHAVLR
LVREAGIQEDTGLRIDFPVSRQDIAELTGTTLHTVSRIVSQWQSKGWVEGGRQSLLVRDLRRLRQIAEGEI

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=205, Percent_Identity=21.9512195121951, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787595, Length=195, Percent_Identity=23.0769230769231, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25737; Mature: 25606

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIIDWALVRSLPVFSGMDDAAFEQLMGLAMPRAVPKGSAVFKQGEEARLFYLLVQGRL
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHEEEEEECCCE
KVMQVTADGQQLIVRVVNPGEFFGFAMALGREDYPGTPLAVVDCSVIAWPMEMMTAFMAS
EEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NPALAVNTMQMIGRRLDAAHSRIREISTQEVEQRVAHAVLRLVREAGIQEDTGLRIDFPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
SRQDIAELTGTTLHTVSRIVSQWQSKGWVEGGRQSLLVRDLRRLRQIAEGEI
CHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAIIDWALVRSLPVFSGMDDAAFEQLMGLAMPRAVPKGSAVFKQGEEARLFYLLVQGRL
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHEEEEEECCCE
KVMQVTADGQQLIVRVVNPGEFFGFAMALGREDYPGTPLAVVDCSVIAWPMEMMTAFMAS
EEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NPALAVNTMQMIGRRLDAAHSRIREISTQEVEQRVAHAVLRLVREAGIQEDTGLRIDFPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
SRQDIAELTGTTLHTVSRIVSQWQSKGWVEGGRQSLLVRDLRRLRQIAEGEI
CHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630321 [H]