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The map label for this gene is norB [H]

Identifier: 225686097

GI number: 225686097

Start: 231186

End: 232535

Strand: Direct

Name: norB [H]

Synonym: BMEA_B0250

Alternate gene names: 225686097

Gene position: 231186-232535 (Clockwise)

Preceding gene: 225686096

Following gene: 225686098

Centisome position: 19.5

GC content: 57.48

Gene sequence:

>1350_bases
ATGAAATACCAAAGCCAGAAAGTGGCAATGCTCTATTTTTATGGAGCTCTTGCCCTGTTCGTCGCGCAAGTTCTGTTCGG
CGTGGTGGCCGGGACAATCTATGTCCTGCCCAACACCTTGTCGGTGCTCTTGCCCTTCAATATCGTCCGCATGATCCATA
CCAACGCCCTCATCGTATGGCTTCTGATGGGCTTCATGGGGTCCACCTATTATCTCCTGCCGGAAGAAACGGAAACGGAG
CTTTACAGCACCAAGCTGGCCGTCATCCAGTTCTGGCTGTTCTTCGTGGCGGCGGGCGTCGCCGTGGCAGGCTATCTCTT
CCATATCCACGAAGGGCGCGAGTTCCTTGAACAGCCCTTCTTCATCAAGGTCGGCATTGTCGTCGTCTGCCTGATATTCC
TGTTCAACATCACGCTGACGGCGCTGAAGGGCCGCAAGACCACCGTGACCAACATTCTCCTGTTCGGTCTCTGGGGGCTT
GCGCTGTTCTTCCTGTTCGCTTTCTACAACCCGATCAATCTCGCGCTCGACAAGCTCTATTGGTGGTATGTGATCCATCT
TTGGGTGGAAGGCGTATGGGAACTCATCATGGCCTCCATCCTGGCCTTCCTGATGATCAAGCTGAACGGCATCGACCGCG
AAGTGGTGGAAAAGTGGCTCTATGTCATCGTTGGCCTGGCGCTCTTCTCCGGTATCCTCGGCACCGGCCACCATTATTAC
TGGATCGGCGCTCCCGGTTACTGGCAGTGGATCGGCTCGCTCTTCTCGACGCTTGAAGTGGCTCCTTTCTTCACCATGGT
CATGTTCACTTTCGTGATGACCTGGCGTGCGGGCCGCAAGCATCCCAACCGCGCCGCTCTCCTGTGGTCGATCGGCTGCT
CGGTGATGGCCTTCTTCGGCGCCGGTGTCTGGGGCTTCCTGCATACGCTCTCTTCGGTCAATTACTACACCCACGGCACG
CAGCTTACCGCTGCCCACGGCCACCTCGCCTTCTTCGGCGCCTATGTCATGCTCAACCTTGCGGTCATGGCTTATGCGAT
CCCCGAAATCCGCGGGCGCGCACCTTATAACCAGTGGCTCTCCATGGTCAGCTTCTGGATGATGTGCACGGCCATGTCGG
TCATGACCTTCGCGCTCACCTTCGCAGGCGTCGTGCAGGTGCACCTCCAGCGTGTGCTGGGCGAAAACTTCATGGAAGTG
CAGGATCAGCTTGCGCTCTTCTATTGGATACGCCTCGGCTCCGGCGTCGTGGTGGTAATCTCGGCTCTGATGTTCGTCTG
GGCGGTGCTCGTCCCCGGCAGGCAACGCAGCCAGAAGCTTTCAGGCTTCGCACAACAGCCTGCCGAATAA

Upstream 100 bases:

>100_bases
GACGATCTTGCGGATTTCCTGGAGTGGACGAGCCGCATCAAGACGCAGAACTGGCCGCCGAACGATGCAGGCTGAGCGTG
GGTGAACGGAGTTTGAAAAT

Downstream 100 bases:

>100_bases
TGATCCGGGAGCCTCGCCTTCGGGCGGGGCTCCATTCTGTCCGATTGCCCGCAGATAAATATTTCTCGATTCCCAAGGAA
AGACCGGCCCATGACCTCCC

Product: Nitric oxide reductase subunit B

Products: NA

Alternate protein names: NOR large subunit; Nitric oxide reductase cytochrome b subunit [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKYQSQKVAMLYFYGALALFVAQVLFGVVAGTIYVLPNTLSVLLPFNIVRMIHTNALIVWLLMGFMGSTYYLLPEETETE
LYSTKLAVIQFWLFFVAAGVAVAGYLFHIHEGREFLEQPFFIKVGIVVVCLIFLFNITLTALKGRKTTVTNILLFGLWGL
ALFFLFAFYNPINLALDKLYWWYVIHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIVGLALFSGILGTGHHYY
WIGAPGYWQWIGSLFSTLEVAPFFTMVMFTFVMTWRAGRKHPNRAALLWSIGCSVMAFFGAGVWGFLHTLSSVNYYTHGT
QLTAAHGHLAFFGAYVMLNLAVMAYAIPEIRGRAPYNQWLSMVSFWMMCTAMSVMTFALTFAGVVQVHLQRVLGENFMEV
QDQLALFYWIRLGSGVVVVISALMFVWAVLVPGRQRSQKLSGFAQQPAE

Sequences:

>Translated_449_residues
MKYQSQKVAMLYFYGALALFVAQVLFGVVAGTIYVLPNTLSVLLPFNIVRMIHTNALIVWLLMGFMGSTYYLLPEETETE
LYSTKLAVIQFWLFFVAAGVAVAGYLFHIHEGREFLEQPFFIKVGIVVVCLIFLFNITLTALKGRKTTVTNILLFGLWGL
ALFFLFAFYNPINLALDKLYWWYVIHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIVGLALFSGILGTGHHYY
WIGAPGYWQWIGSLFSTLEVAPFFTMVMFTFVMTWRAGRKHPNRAALLWSIGCSVMAFFGAGVWGFLHTLSSVNYYTHGT
QLTAAHGHLAFFGAYVMLNLAVMAYAIPEIRGRAPYNQWLSMVSFWMMCTAMSVMTFALTFAGVVQVHLQRVLGENFMEV
QDQLALFYWIRLGSGVVVVISALMFVWAVLVPGRQRSQKLSGFAQQPAE
>Mature_449_residues
MKYQSQKVAMLYFYGALALFVAQVLFGVVAGTIYVLPNTLSVLLPFNIVRMIHTNALIVWLLMGFMGSTYYLLPEETETE
LYSTKLAVIQFWLFFVAAGVAVAGYLFHIHEGREFLEQPFFIKVGIVVVCLIFLFNITLTALKGRKTTVTNILLFGLWGL
ALFFLFAFYNPINLALDKLYWWYVIHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIVGLALFSGILGTGHHYY
WIGAPGYWQWIGSLFSTLEVAPFFTMVMFTFVMTWRAGRKHPNRAALLWSIGCSVMAFFGAGVWGFLHTLSSVNYYTHGT
QLTAAHGHLAFFGAYVMLNLAVMAYAIPEIRGRAPYNQWLSMVSFWMMCTAMSVMTFALTFAGVVQVHLQRVLGENFMEV
QDQLALFYWIRLGSGVVVVISALMFVWAVLVPGRQRSQKLSGFAQQPAE

Specific function: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen red

COG id: COG3256

COG function: function code P; Nitric oxide reductase large subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heme-copper respiratory oxidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000883 [H]

Pfam domain/function: PF00115 COX1 [H]

EC number: =1.7.99.7 [H]

Molecular weight: Translated: 50959; Mature: 50959

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50855 COX1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQSQKVAMLYFYGALALFVAQVLFGVVAGTIYVLPNTLSVLLPFNIVRMIHTNALIVW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMGFMGSTYYLLPEETETELYSTKLAVIQFWLFFVAAGVAVAGYLFHIHEGREFLEQPF
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FIKVGIVVVCLIFLFNITLTALKGRKTTVTNILLFGLWGLALFFLFAFYNPINLALDKLY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
WWYVIHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIVGLALFSGILGTGHHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WIGAPGYWQWIGSLFSTLEVAPFFTMVMFTFVMTWRAGRKHPNRAALLWSIGCSVMAFFG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AGVWGFLHTLSSVNYYTHGTQLTAAHGHLAFFGAYVMLNLAVMAYAIPEIRGRAPYNQWL
HHHHHHHHHHHHCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SMVSFWMMCTAMSVMTFALTFAGVVQVHLQRVLGENFMEVQDQLALFYWIRLGSGVVVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHH
SALMFVWAVLVPGRQRSQKLSGFAQQPAE
HHHHHHHHHHCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKYQSQKVAMLYFYGALALFVAQVLFGVVAGTIYVLPNTLSVLLPFNIVRMIHTNALIVW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMGFMGSTYYLLPEETETELYSTKLAVIQFWLFFVAAGVAVAGYLFHIHEGREFLEQPF
HHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FIKVGIVVVCLIFLFNITLTALKGRKTTVTNILLFGLWGLALFFLFAFYNPINLALDKLY
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
WWYVIHLWVEGVWELIMASILAFLMIKLNGIDREVVEKWLYVIVGLALFSGILGTGHHYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WIGAPGYWQWIGSLFSTLEVAPFFTMVMFTFVMTWRAGRKHPNRAALLWSIGCSVMAFFG
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
AGVWGFLHTLSSVNYYTHGTQLTAAHGHLAFFGAYVMLNLAVMAYAIPEIRGRAPYNQWL
HHHHHHHHHHHHCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
SMVSFWMMCTAMSVMTFALTFAGVVQVHLQRVLGENFMEVQDQLALFYWIRLGSGVVVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHH
SALMFVWAVLVPGRQRSQKLSGFAQQPAE
HHHHHHHHHHCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7508388; 2542222; 9521721 [H]