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The map label for this gene is apbE [H]

Identifier: 225686085

GI number: 225686085

Start: 220942

End: 221919

Strand: Direct

Name: apbE [H]

Synonym: BMEA_B0234

Alternate gene names: 225686085

Gene position: 220942-221919 (Clockwise)

Preceding gene: 225686084

Following gene: 225686087

Centisome position: 18.64

GC content: 61.45

Gene sequence:

>978_bases
ATGTCGAAGATGTTTACTGATCTCAAGCGCCATGGATTGAACGGCCCCACCATGCGCACGCGCTGGTCGGCAATTTTTTA
CGCCGCTGCCGATTTCGACATCGCTCCCTTGCGGCGCGCGCTGCAACAGGCGGTGGATGAGGTGGACATGCAGATGTCCA
CCTGGAAGCCGGACAGCGATCTTATGCGCCTCAACCGCGCGCCGGTGGGTGTGTGGGTTGAGGTTCCAGCCCGTTTGATG
GAGGTCCTGCGTCTTGCAATCGAGATAGGGCGGGCCACGGGTGGCGCTTTCGATGCCGCCATGGGCGATGCGGTGAAGGC
ATGGGGTTTCGGCCCCATGGAAGCCGATCAAAGGCTCATAAGCGAGGCTCTCGCTGGCCCGCGCGTTGCGGCGCATGATG
CGCTGGAACTCGATATTTCCGCTATGCGGGTGCGCAAGCGTGCAGCTGTTACGCTTGATCTCAACGGCATCGCCAAGGGC
TATGGCGTGGATCGGCTGGCCGACACGCTGGAGAGCTTCGGCATCCATTCCGGTCTGGTGGGCATAGATGGCGAAATGCG
CGCGCTTGAACTTCGACCGGATGGCACGCCATGGGCAATTGCTGTCGAAATGCCGGATTATAACCGCCGCGCGCCGCATT
CGGTGCTGGCGCTGCATAATGGCGCCGTCGCCACATCCGGCGATTATCGGCACTGGGTCGTGGCGGGCGGGCACAGGCTG
TCGCACACGATGGATCCGCATCGTGGCGCTCCGCTTTCCACGCCGCCTGCTTCCGTTACCGTTGTTGCAAAGACCTGCGC
GCAAGCAGATGCCTTTGCAACGGCGCTGATGGTGCTCGGCCCCATAGCCGGGTCAAAGCTTGCGCAAAGCCTCGGTCTCG
ATGTTTTGTTTCTGTTGCGTGATGGTGAAACCATTCGCCCGCAGCCGGTCGGCGCCCTGTTTTCAGGCCACCCTTATCCG
CTGCAAAAACTCGGATAG

Upstream 100 bases:

>100_bases
TGTGGCGGACGCGACATGGCGGCGGGTGTTTCCGAAGCCCTTGCCGATATTCTGGCTCCAGCCGGATTGACGCCTGCCGT
TCTGAAGGCGGAGGGGCGCT

Downstream 100 bases:

>100_bases
CGGCAAAATAAATTAGAGCGGTCGAGCAAAAGTGCGCCGCGCTTACGCCGCCTGCTTTTCTTCAAAATATTCAAACCGGT
TCTTGCCTTTGGATTTGGCG

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSKMFTDLKRHGLNGPTMRTRWSAIFYAAADFDIAPLRRALQQAVDEVDMQMSTWKPDSDLMRLNRAPVGVWVEVPARLM
EVLRLAIEIGRATGGAFDAAMGDAVKAWGFGPMEADQRLISEALAGPRVAAHDALELDISAMRVRKRAAVTLDLNGIAKG
YGVDRLADTLESFGIHSGLVGIDGEMRALELRPDGTPWAIAVEMPDYNRRAPHSVLALHNGAVATSGDYRHWVVAGGHRL
SHTMDPHRGAPLSTPPASVTVVAKTCAQADAFATALMVLGPIAGSKLAQSLGLDVLFLLRDGETIRPQPVGALFSGHPYP
LQKLG

Sequences:

>Translated_325_residues
MSKMFTDLKRHGLNGPTMRTRWSAIFYAAADFDIAPLRRALQQAVDEVDMQMSTWKPDSDLMRLNRAPVGVWVEVPARLM
EVLRLAIEIGRATGGAFDAAMGDAVKAWGFGPMEADQRLISEALAGPRVAAHDALELDISAMRVRKRAAVTLDLNGIAKG
YGVDRLADTLESFGIHSGLVGIDGEMRALELRPDGTPWAIAVEMPDYNRRAPHSVLALHNGAVATSGDYRHWVVAGGHRL
SHTMDPHRGAPLSTPPASVTVVAKTCAQADAFATALMVLGPIAGSKLAQSLGLDVLFLLRDGETIRPQPVGALFSGHPYP
LQKLG
>Mature_324_residues
SKMFTDLKRHGLNGPTMRTRWSAIFYAAADFDIAPLRRALQQAVDEVDMQMSTWKPDSDLMRLNRAPVGVWVEVPARLME
VLRLAIEIGRATGGAFDAAMGDAVKAWGFGPMEADQRLISEALAGPRVAAHDALELDISAMRVRKRAAVTLDLNGIAKGY
GVDRLADTLESFGIHSGLVGIDGEMRALELRPDGTPWAIAVEMPDYNRRAPHSVLALHNGAVATSGDYRHWVVAGGHRLS
HTMDPHRGAPLSTPPASVTVVAKTCAQADAFATALMVLGPIAGSKLAQSLGLDVLFLLRDGETIRPQPVGALFSGHPYPL
QKLG

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=299, Percent_Identity=31.1036789297659, Blast_Score=150, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34969; Mature: 34838

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMFTDLKRHGLNGPTMRTRWSAIFYAAADFDIAPLRRALQQAVDEVDMQMSTWKPDSD
CCHHHHHHHHCCCCCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
LMRLNRAPVGVWVEVPARLMEVLRLAIEIGRATGGAFDAAMGDAVKAWGFGPMEADQRLI
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
SEALAGPRVAAHDALELDISAMRVRKRAAVTLDLNGIAKGYGVDRLADTLESFGIHSGLV
HHHHCCCCEECCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEE
GIDGEMRALELRPDGTPWAIAVEMPDYNRRAPHSVLALHNGAVATSGDYRHWVVAGGHRL
ECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEECCCCEEEEEECCCCC
SHTMDPHRGAPLSTPPASVTVVAKTCAQADAFATALMVLGPIAGSKLAQSLGLDVLFLLR
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEE
DGETIRPQPVGALFSGHPYPLQKLG
CCCCCCCCCCCHHHCCCCCCHHHCC
>Mature Secondary Structure 
SKMFTDLKRHGLNGPTMRTRWSAIFYAAADFDIAPLRRALQQAVDEVDMQMSTWKPDSD
CHHHHHHHHCCCCCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
LMRLNRAPVGVWVEVPARLMEVLRLAIEIGRATGGAFDAAMGDAVKAWGFGPMEADQRLI
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
SEALAGPRVAAHDALELDISAMRVRKRAAVTLDLNGIAKGYGVDRLADTLESFGIHSGLV
HHHHCCCCEECCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEE
GIDGEMRALELRPDGTPWAIAVEMPDYNRRAPHSVLALHNGAVATSGDYRHWVVAGGHRL
ECCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCCEEECCCCEEEEEECCCCC
SHTMDPHRGAPLSTPPASVTVVAKTCAQADAFATALMVLGPIAGSKLAQSLGLDVLFLLR
CCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEE
DGETIRPQPVGALFSGHPYPLQKLG
CCCCCCCCCCCHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]