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The map label for this gene is pmbA [H]

Identifier: 225686064

GI number: 225686064

Start: 197306

End: 198649

Strand: Direct

Name: pmbA [H]

Synonym: BMEA_B0211

Alternate gene names: 225686064

Gene position: 197306-198649 (Clockwise)

Preceding gene: 225686062

Following gene: 225686065

Centisome position: 16.64

GC content: 61.76

Gene sequence:

>1344_bases
ATGATTTCAGATAATTCGGCAGATAAACTCGTAGAACGCGCTGCCGAACTGGTGGCTGCTGCAAAGCGCGCTGGTGCGGA
CGAGGTTGACGCGGTTGTGATGCGCGCGCGTTCTGTCAGCGTGTCTGTCAGGCTTGGCAAGGTTGAGGGAACCGAATCGT
CAGAGAGCGACGATTTCGCGCTGCGCGTATTTGTCGGGCGTCGCATTGCCAGCGTTTCGGCCAATGCAGGCGCCGACCCG
CACCGCCTTGCCGAACGTGTGGTGGCCATGGCGCGGGTGGCGCCGGAAGATCCTTATGAACGATTGGCCGATCCGTCGCT
TCTGGTAAAATCGCCGCGCGATCTCGACCTGTTCGATGCGACCGAGATCGATACCGCGCGCCTGACCGAAGACGCGCTTG
CAATGGAGGCGGCGGCTCTTGCTGTTTCAGGCGTAATCAATTCCGGTGGTGCGGGTGCATCACGGAGTCTTGGCGGGATG
GTTCTTGTCACCTCCACCGGCTTTTCCGGCCATTATGCGGCCACGCGATTCGGGCGTTCGGTTTCGGCCATTGCGGGCGA
AGGGACCGGCATGGAGCGCGATTACGATTTCAGTTCGCGCCTGCATTTTGCCGATCTCGACGCGCCGGAAGCTATCGGGC
GCAGCGCCGGGGAGCGCGCCGTGCGCAGGCTGGGCGCCCGGCAGGCAAAGACCGGCCCGGTGACCGTGGTGTATGATCCG
CGTTTGGCGCGCGGCATTGCCGGGCATCTGGCAAGCGCCATCAATGGCGCATCGGTTGCCCGCAAGACGAGCTTTCTGCG
CAACAGTCTGGGCAAGCCGGTTTTGAAACAGGGCATCAACGTCACCGACAATCCGCTGCGTGTGCGCGGCCCGTCTTCAC
GTCCTTTCGATGGCGAGGGCATTGAAGGCCAGCCGCTTACGATGGTTGAAGACGGTGTGTTGCAGCATTGGCTGCTGTCC
GGCTCAAGTGCGCGTGAGCTTGGCCTTCAGGGCAACGGGCGCGGCGTGCGCTCCGGTTCGGGCGTCACGCCAGCCTCCAC
CAATTTTGCCATCGAACCCGGTACCCAATCACCGGAAGAACTGATCCGCGCCGTTGGCACGGGCTTCTATGTAACGGAAG
TGTTCGGGCAGGGCGTGGACATGATTAGCGGCCAATATAGCCGCGGCGCATCCGGTTTCTGGATCGAGAATGGCGAGCTT
GCTTACCCGGTGAGCGAAGTCACCATCGCGTCGAACCTGAAGGACATGTTCCTCAATATGACGCCTGCGTCCGACATCGA
CCGCAATTTTGGCATGGCTGCGCCGACCCTCGTGATCGAAGGCATGACCCTTGCCGGAAACTGA

Upstream 100 bases:

>100_bases
AACAATTTGACGATTTTTTTTGGCAGGTCCCGATTGGGGATAGTCGTGGATCAGGGCTTGCAGCCAGGCGGCAGTGAGCC
TGCAACGTTTGGATTGTATA

Downstream 100 bases:

>100_bases
CAAATATAGCGATATCGGCACGGAACTTGATTTGCTGCGAGAGGCGGCGCGCGAGGCCGGGCGCATCGCCATGCATTATT
TCGGGCGCTCGCCGGAGGTC

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MISDNSADKLVERAAELVAAAKRAGADEVDAVVMRARSVSVSVRLGKVEGTESSESDDFALRVFVGRRIASVSANAGADP
HRLAERVVAMARVAPEDPYERLADPSLLVKSPRDLDLFDATEIDTARLTEDALAMEAAALAVSGVINSGGAGASRSLGGM
VLVTSTGFSGHYAATRFGRSVSAIAGEGTGMERDYDFSSRLHFADLDAPEAIGRSAGERAVRRLGARQAKTGPVTVVYDP
RLARGIAGHLASAINGASVARKTSFLRNSLGKPVLKQGINVTDNPLRVRGPSSRPFDGEGIEGQPLTMVEDGVLQHWLLS
GSSARELGLQGNGRGVRSGSGVTPASTNFAIEPGTQSPEELIRAVGTGFYVTEVFGQGVDMISGQYSRGASGFWIENGEL
AYPVSEVTIASNLKDMFLNMTPASDIDRNFGMAAPTLVIEGMTLAGN

Sequences:

>Translated_447_residues
MISDNSADKLVERAAELVAAAKRAGADEVDAVVMRARSVSVSVRLGKVEGTESSESDDFALRVFVGRRIASVSANAGADP
HRLAERVVAMARVAPEDPYERLADPSLLVKSPRDLDLFDATEIDTARLTEDALAMEAAALAVSGVINSGGAGASRSLGGM
VLVTSTGFSGHYAATRFGRSVSAIAGEGTGMERDYDFSSRLHFADLDAPEAIGRSAGERAVRRLGARQAKTGPVTVVYDP
RLARGIAGHLASAINGASVARKTSFLRNSLGKPVLKQGINVTDNPLRVRGPSSRPFDGEGIEGQPLTMVEDGVLQHWLLS
GSSARELGLQGNGRGVRSGSGVTPASTNFAIEPGTQSPEELIRAVGTGFYVTEVFGQGVDMISGQYSRGASGFWIENGEL
AYPVSEVTIASNLKDMFLNMTPASDIDRNFGMAAPTLVIEGMTLAGN
>Mature_447_residues
MISDNSADKLVERAAELVAAAKRAGADEVDAVVMRARSVSVSVRLGKVEGTESSESDDFALRVFVGRRIASVSANAGADP
HRLAERVVAMARVAPEDPYERLADPSLLVKSPRDLDLFDATEIDTARLTEDALAMEAAALAVSGVINSGGAGASRSLGGM
VLVTSTGFSGHYAATRFGRSVSAIAGEGTGMERDYDFSSRLHFADLDAPEAIGRSAGERAVRRLGARQAKTGPVTVVYDP
RLARGIAGHLASAINGASVARKTSFLRNSLGKPVLKQGINVTDNPLRVRGPSSRPFDGEGIEGQPLTMVEDGVLQHWLLS
GSSARELGLQGNGRGVRSGSGVTPASTNFAIEPGTQSPEELIRAVGTGFYVTEVFGQGVDMISGQYSRGASGFWIENGEL
AYPVSEVTIASNLKDMFLNMTPASDIDRNFGMAAPTLVIEGMTLAGN

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=444, Percent_Identity=36.036036036036, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789640, Length=278, Percent_Identity=27.6978417266187, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 46828; Mature: 46828

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISDNSADKLVERAAELVAAAKRAGADEVDAVVMRARSVSVSVRLGKVEGTESSESDDFA
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCEE
LRVFVGRRIASVSANAGADPHRLAERVVAMARVAPEDPYERLADPSLLVKSPRDLDLFDA
EEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCCCCCCCCC
TEIDTARLTEDALAMEAAALAVSGVINSGGAGASRSLGGMVLVTSTGFSGHYAATRFGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCC
VSAIAGEGTGMERDYDFSSRLHFADLDAPEAIGRSAGERAVRRLGARQAKTGPVTVVYDP
HHHHCCCCCCCCCCCCHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
RLARGIAGHLASAINGASVARKTSFLRNSLGKPVLKQGINVTDNPLRVRGPSSRPFDGEG
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCCCCCCCCCC
IEGQPLTMVEDGVLQHWLLSGSSARELGLQGNGRGVRSGSGVTPASTNFAIEPGTQSPEE
CCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHH
LIRAVGTGFYVTEVFGQGVDMISGQYSRGASGFWIENGELAYPVSEVTIASNLKDMFLNM
HHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHEEC
TPASDIDRNFGMAAPTLVIEGMTLAGN
CCCHHHHCCCCCCCCCCEEECEEECCC
>Mature Secondary Structure
MISDNSADKLVERAAELVAAAKRAGADEVDAVVMRARSVSVSVRLGKVEGTESSESDDFA
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCEE
LRVFVGRRIASVSANAGADPHRLAERVVAMARVAPEDPYERLADPSLLVKSPRDLDLFDA
EEEEECCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCCCCCCCCC
TEIDTARLTEDALAMEAAALAVSGVINSGGAGASRSLGGMVLVTSTGFSGHYAATRFGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCC
VSAIAGEGTGMERDYDFSSRLHFADLDAPEAIGRSAGERAVRRLGARQAKTGPVTVVYDP
HHHHCCCCCCCCCCCCHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
RLARGIAGHLASAINGASVARKTSFLRNSLGKPVLKQGINVTDNPLRVRGPSSRPFDGEG
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEECCCCCCCCCCC
IEGQPLTMVEDGVLQHWLLSGSSARELGLQGNGRGVRSGSGVTPASTNFAIEPGTQSPEE
CCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHH
LIRAVGTGFYVTEVFGQGVDMISGQYSRGASGFWIENGELAYPVSEVTIASNLKDMFLNM
HHHHHCCCHHHHHHHHCCHHHHCCCCCCCCCCEEEECCCEECCHHHHHHHHHHHHHHEEC
TPASDIDRNFGMAAPTLVIEGMTLAGN
CCCHHHHCCCCCCCCCCEEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]