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The map label for this gene is citE [C]

Identifier: 225686025

GI number: 225686025

Start: 153071

End: 153895

Strand: Direct

Name: citE [C]

Synonym: BMEA_B0169

Alternate gene names: 225686025

Gene position: 153071-153895 (Clockwise)

Preceding gene: 225686024

Following gene: 225686026

Centisome position: 12.91

GC content: 59.39

Gene sequence:

>825_bases
ATGGTGAGTATTGCATCGATACGAGCGCCGTTATTCGTACCGGCGGACAGGCCAGACCGGTTTTCCAAGGCGGCAGCGTC
GGGCACCGATTGCGTTATCCTTGATCTTGAGGATGCCGTTGCCCCAAAGGCGAAAGATGCGGCGCGCGCCGCATTGGACA
CTTCCTTCACGGATTTACCTGTGATGGTGCGCATCAATCCCCATGGAACACCATGGCATGAGGCGGATATGGCCGCTGTG
GCCCGCTTAGACGTAGCGGGCGTGATCCTGCCCAAGGCCGAGACTGCGGAGCGCATCGAGGCGGCTGCAAGGATGCTCGA
CTTGCCGCTGATTGCTCTGGTCGAAACTGCGCGCGGCCTTGCCAATGCGCGTTCAATTGCAGCGGCTTCGGGTGTGGTGC
GGCTGGCGTTCGGCTCGATAGATTTCTGCGCCGATTTGGCCTGTGCCCATATGCGCGAAATTCTGCTGCCTGCACGCGTG
GAACTGGTTATGGCTTCGCGCCTTGCGGGTATCGCAGCCCCCATCGACGGTGTTACGCCCGGGTTTAATGATCCGGTTAT
GTGCCATCAGGATGCCGCCCATGCGCGCGCGCTGGGGATGATGGGAAAATTGTGCATCCATCCCAGACAGATATCCGAAG
TTCTGCGTGCATTCGCCTGGTCCGATCAGGAGATAGACTGGGCGCGCCGCGTTCTGGCTTCAGGCGAAGGGGCTATTTCC
ATAGACGGCATGATGGTTGATGAGCCGGTGCGTATCCGTGCACGCACCATTCTTGAGCAAGCCGATATTGGAGCGGCTCC
GGTTTTGACAAAACCAACGGGCTGA

Upstream 100 bases:

>100_bases
ACGATGCGGACTTCACCCTCAATGCGATTGATGATGGCGAGGGGCTCAAGCTTTGGACGTCTTATGCCAATGGTCCGGTG
GCTATGGAGGCGCGGGCGCT

Downstream 100 bases:

>100_bases
TCGTCTCGGTATTTGATGGTGCATTTGCAGCCATGCATGGAAGGATCGGCTATGGGGCAGATGAACTTGCTCCGGCATTG
GCCATCAAGCCGGAGTTTGA

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MVSIASIRAPLFVPADRPDRFSKAAASGTDCVILDLEDAVAPKAKDAARAALDTSFTDLPVMVRINPHGTPWHEADMAAV
ARLDVAGVILPKAETAERIEAAARMLDLPLIALVETARGLANARSIAAASGVVRLAFGSIDFCADLACAHMREILLPARV
ELVMASRLAGIAAPIDGVTPGFNDPVMCHQDAAHARALGMMGKLCIHPRQISEVLRAFAWSDQEIDWARRVLASGEGAIS
IDGMMVDEPVRIRARTILEQADIGAAPVLTKPTG

Sequences:

>Translated_274_residues
MVSIASIRAPLFVPADRPDRFSKAAASGTDCVILDLEDAVAPKAKDAARAALDTSFTDLPVMVRINPHGTPWHEADMAAV
ARLDVAGVILPKAETAERIEAAARMLDLPLIALVETARGLANARSIAAASGVVRLAFGSIDFCADLACAHMREILLPARV
ELVMASRLAGIAAPIDGVTPGFNDPVMCHQDAAHARALGMMGKLCIHPRQISEVLRAFAWSDQEIDWARRVLASGEGAIS
IDGMMVDEPVRIRARTILEQADIGAAPVLTKPTG
>Mature_274_residues
MVSIASIRAPLFVPADRPDRFSKAAASGTDCVILDLEDAVAPKAKDAARAALDTSFTDLPVMVRINPHGTPWHEADMAAV
ARLDVAGVILPKAETAERIEAAARMLDLPLIALVETARGLANARSIAAASGVVRLAFGSIDFCADLACAHMREILLPARV
ELVMASRLAGIAAPIDGVTPGFNDPVMCHQDAAHARALGMMGKLCIHPRQISEVLRAFAWSDQEIDWARRVLASGEGAIS
IDGMMVDEPVRIRARTILEQADIGAAPVLTKPTG

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=285, Percent_Identity=30.1754385964912, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI87081764, Length=283, Percent_Identity=33.2155477031802, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17557458, Length=294, Percent_Identity=27.5510204081633, Blast_Score=81, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 29008; Mature: 29008

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSIASIRAPLFVPADRPDRFSKAAASGTDCVILDLEDAVAPKAKDAARAALDTSFTDLP
CCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECHHHCCCCHHHHHHHHHCCCCCCCE
VMVRINPHGTPWHEADMAAVARLDVAGVILPKAETAERIEAAARMLDLPLIALVETARGL
EEEEECCCCCCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ANARSIAAASGVVRLAFGSIDFCADLACAHMREILLPARVELVMASRLAGIAAPIDGVTP
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GFNDPVMCHQDAAHARALGMMGKLCIHPRQISEVLRAFAWSDQEIDWARRVLASGEGAIS
CCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
IDGMMVDEPVRIRARTILEQADIGAAPVLTKPTG
ECCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVSIASIRAPLFVPADRPDRFSKAAASGTDCVILDLEDAVAPKAKDAARAALDTSFTDLP
CCCCCCCCCCEEEECCCCHHHHHHHCCCCCEEEEECHHHCCCCHHHHHHHHHCCCCCCCE
VMVRINPHGTPWHEADMAAVARLDVAGVILPKAETAERIEAAARMLDLPLIALVETARGL
EEEEECCCCCCCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ANARSIAAASGVVRLAFGSIDFCADLACAHMREILLPARVELVMASRLAGIAAPIDGVTP
HHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GFNDPVMCHQDAAHARALGMMGKLCIHPRQISEVLRAFAWSDQEIDWARRVLASGEGAIS
CCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEE
IDGMMVDEPVRIRARTILEQADIGAAPVLTKPTG
ECCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]