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The map label for this gene is dhbF [H]

Identifier: 225685885

GI number: 225685885

Start: 15071

End: 16480

Strand: Direct

Name: dhbF [H]

Synonym: BMEA_B0019

Alternate gene names: 225685885

Gene position: 15071-16480 (Clockwise)

Preceding gene: 225685880

Following gene: 225685889

Centisome position: 1.27

GC content: 58.72

Gene sequence:

>1410_bases
TTGGTCCCAATTTGTCAACCGGGTGATTGGCCGACGCTGAACGAAGCTTGCCGAGCCGAGAGGAATGAGATGGCCAGGAT
AGATTTTACAGATGGCTGGATGCCGCTGACATTGCCCCAGCTCGATTTCTGGGAAGAGTTTTCCTTCCACCCCGACCAGC
CGGTCTCCACGGTCGCGCATTGCGTGGAAATCGAAGGCGATGTGGATGTGGAAAAATTGCTGCGCGCCATTTCGCAGACC
GTCCGTGAGGCGGAGGTTCTTTCCATCCGCTTTTCCGATGAAAAGGAGCAGGGGCGTCCGGTGCAATTATGCGACCCGGC
GCATATGCCGGACGTGGATTTTGTTGATCTCAGCGCCTTTCCCGATCCGCGGGCAGAGGCTGGGCGGCGTATGGAAACCG
ATCTGTCCGCACCGCTTGATCTGCGCCGGGACAGGATGTCCGCCCATGCGCTTTATCGCATCGGCGCGCGCCAATATATC
TGGTATATCCGCGCCCATCACATCATTATCGACGGTTACGGGCTGGCGCTGATCGAACAGCGTTGCGGCCAGCTTTACAG
CCATTTTTGCGGCCAGGGCGAAAGTGGCCACCCCTTCCATCCTTTTGCAAGCTTCATTCATGAGGAGGACGCTTATCGGG
CAAGCCCCCGCTGGGAGGCCGACCGCAAATTCTGGCAGGATTATCTGGACGGGCCTGTGCGCCTTCCCATCCTCGATCGG
GGCGCGGAAGATTATGGCGAGGCGGGCTATCATCATGACGTCGCCTTTCGCGACGATATTTCCATAGGCCTTCGCGCGAT
GGCAAAAGCCACGGATATCGGCTGGCCCGATCTTCTGGTGCTTTTATCGGGGCTCTATCTCTCGCGGATGCTTCCGATGC
AGGGAGAGGACGAGGGGCTGACGCTGTGGCTGCCTTTCATGAGCCGCTGGGGCAGCGTGGGCGCACATATGCCGGGGCTT
CTGGTCAATATCCTGCCGTTCCATCTTGAATTCGATTCCGGCGAAACATTGTTCTCTGCGTTACGGCGCAATATGGACAT
GCTGAAAAAGCACCGGCTGCACGGGCGCTATCGCATCGAACAGATTTCCGCCGACCGCGGCCTCGTCAGTGGAAGCCGCT
TTTTCTTCTCGCCGCTGATTAATGTGCTGCCATTCAGCACGCCGCAATTTGCAGGTTGCCGGGTTGCGCGTGAGGTTCTG
GCCAATGGCCCCGGCGACGGCTTCAACCTGACCTATCGTGCCGATGTCGATGGCGCTGGCCTTACCTTGAAAATCGAAGC
GGCATCGAACATGATGGATGCGCAAACCTTTGCCCGCATTGCGGCGGGCCTTCCGGCCTTTCTCAAGGCAAGCCTTGCGC
CCCATCTCATCGAGCGTCCGGTGAGCGATCTTCGCCGCCGTTTGCCATGA

Upstream 100 bases:

>100_bases
GTTGGGTATTTCAGGGCGCTCGTTCTCACGGGGAGTCTCCTTGGCGTCGTTGCCGAAATTAATACTTGAAAGAACCAGTC
ACGTTTTATACTTGATTTAA

Downstream 100 bases:

>100_bases
TGTGAAAATGGGCGGCGGCCTTAAAAGACAGCCGCCCGATCCGGTACTTATATTCCGCCGTACCAGTCATATCCCTGGTC
TTCCCAATAGCCGCCCCGGC

Product: Linear gramicidin synthetase subunit B

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MVPICQPGDWPTLNEACRAERNEMARIDFTDGWMPLTLPQLDFWEEFSFHPDQPVSTVAHCVEIEGDVDVEKLLRAISQT
VREAEVLSIRFSDEKEQGRPVQLCDPAHMPDVDFVDLSAFPDPRAEAGRRMETDLSAPLDLRRDRMSAHALYRIGARQYI
WYIRAHHIIIDGYGLALIEQRCGQLYSHFCGQGESGHPFHPFASFIHEEDAYRASPRWEADRKFWQDYLDGPVRLPILDR
GAEDYGEAGYHHDVAFRDDISIGLRAMAKATDIGWPDLLVLLSGLYLSRMLPMQGEDEGLTLWLPFMSRWGSVGAHMPGL
LVNILPFHLEFDSGETLFSALRRNMDMLKKHRLHGRYRIEQISADRGLVSGSRFFFSPLINVLPFSTPQFAGCRVAREVL
ANGPGDGFNLTYRADVDGAGLTLKIEAASNMMDAQTFARIAAGLPAFLKASLAPHLIERPVSDLRRRLP

Sequences:

>Translated_469_residues
MVPICQPGDWPTLNEACRAERNEMARIDFTDGWMPLTLPQLDFWEEFSFHPDQPVSTVAHCVEIEGDVDVEKLLRAISQT
VREAEVLSIRFSDEKEQGRPVQLCDPAHMPDVDFVDLSAFPDPRAEAGRRMETDLSAPLDLRRDRMSAHALYRIGARQYI
WYIRAHHIIIDGYGLALIEQRCGQLYSHFCGQGESGHPFHPFASFIHEEDAYRASPRWEADRKFWQDYLDGPVRLPILDR
GAEDYGEAGYHHDVAFRDDISIGLRAMAKATDIGWPDLLVLLSGLYLSRMLPMQGEDEGLTLWLPFMSRWGSVGAHMPGL
LVNILPFHLEFDSGETLFSALRRNMDMLKKHRLHGRYRIEQISADRGLVSGSRFFFSPLINVLPFSTPQFAGCRVAREVL
ANGPGDGFNLTYRADVDGAGLTLKIEAASNMMDAQTFARIAAGLPAFLKASLAPHLIERPVSDLRRRLP
>Mature_469_residues
MVPICQPGDWPTLNEACRAERNEMARIDFTDGWMPLTLPQLDFWEEFSFHPDQPVSTVAHCVEIEGDVDVEKLLRAISQT
VREAEVLSIRFSDEKEQGRPVQLCDPAHMPDVDFVDLSAFPDPRAEAGRRMETDLSAPLDLRRDRMSAHALYRIGARQYI
WYIRAHHIIIDGYGLALIEQRCGQLYSHFCGQGESGHPFHPFASFIHEEDAYRASPRWEADRKFWQDYLDGPVRLPILDR
GAEDYGEAGYHHDVAFRDDISIGLRAMAKATDIGWPDLLVLLSGLYLSRMLPMQGEDEGLTLWLPFMSRWGSVGAHMPGL
LVNILPFHLEFDSGETLFSALRRNMDMLKKHRLHGRYRIEQISADRGLVSGSRFFFSPLINVLPFSTPQFAGCRVAREVL
ANGPGDGFNLTYRADVDGAGLTLKIEAASNMMDAQTFARIAAGLPAFLKASLAPHLIERPVSDLRRRLP

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786801, Length=437, Percent_Identity=27.9176201372998, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 53017; Mature: 53017

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPICQPGDWPTLNEACRAERNEMARIDFTDGWMPLTLPQLDFWEEFSFHPDQPVSTVAH
CCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEECCCCHHHHHCCCCCCCHHHHHHH
CVEIEGDVDVEKLLRAISQTVREAEVLSIRFSDEKEQGRPVQLCDPAHMPDVDFVDLSAF
HHHCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEECCCCCCCCCEEECCCC
PDPRAEAGRRMETDLSAPLDLRRDRMSAHALYRIGARQYIWYIRAHHIIIDGYGLALIEQ
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCHHHHHHH
RCGQLYSHFCGQGESGHPFHPFASFIHEEDAYRASPRWEADRKFWQDYLDGPVRLPILDR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
GAEDYGEAGYHHDVAFRDDISIGLRAMAKATDIGWPDLLVLLSGLYLSRMLPMQGEDEGL
CCHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCE
TLWLPFMSRWGSVGAHMPGLLVNILPFHLEFDSGETLFSALRRNMDMLKKHRLHGRYRIE
EEEEHHHHHCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEE
QISADRGLVSGSRFFFSPLINVLPFSTPQFAGCRVAREVLANGPGDGFNLTYRADVDGAG
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
LTLKIEAASNMMDAQTFARIAAGLPAFLKASLAPHLIERPVSDLRRRLP
EEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MVPICQPGDWPTLNEACRAERNEMARIDFTDGWMPLTLPQLDFWEEFSFHPDQPVSTVAH
CCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEECCCCHHHHHCCCCCCCHHHHHHH
CVEIEGDVDVEKLLRAISQTVREAEVLSIRFSDEKEQGRPVQLCDPAHMPDVDFVDLSAF
HHHCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCEEEECCCCCCCCCEEECCCC
PDPRAEAGRRMETDLSAPLDLRRDRMSAHALYRIGARQYIWYIRAHHIIIDGYGLALIEQ
CCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCHHHHHHH
RCGQLYSHFCGQGESGHPFHPFASFIHEEDAYRASPRWEADRKFWQDYLDGPVRLPILDR
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECC
GAEDYGEAGYHHDVAFRDDISIGLRAMAKATDIGWPDLLVLLSGLYLSRMLPMQGEDEGL
CCHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCE
TLWLPFMSRWGSVGAHMPGLLVNILPFHLEFDSGETLFSALRRNMDMLKKHRLHGRYRIE
EEEEHHHHHCCCCCCCCHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEE
QISADRGLVSGSRFFFSPLINVLPFSTPQFAGCRVAREVLANGPGDGFNLTYRADVDGAG
HHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
LTLKIEAASNMMDAQTFARIAAGLPAFLKASLAPHLIERPVSDLRRRLP
EEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]