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The map label for this gene is pdhB [H]

Identifier: 225630112

GI number: 225630112

Start: 306817

End: 307815

Strand: Direct

Name: pdhB [H]

Synonym: WRi_002920

Alternate gene names: 225630112

Gene position: 306817-307815 (Clockwise)

Preceding gene: 225630111

Following gene: 225630117

Centisome position: 21.22

GC content: 40.04

Gene sequence:

>999_bases
ATGGCAACCTTAAGTGTAAGAGAAGCTTTATGCACAGCAATTAGAGAAGAAATGCAAAACGACTCTGATGTGCTTATCAT
GGGTGAAGAAGTTGCAGAGTATGATGGTGCTTATAAAGTAACGAAAGGATTACTGAAAGAGTTTGGAGAAAACAGAGTAG
TTGATACGCCTATTACCGAACATGGATTTGCTGGCCTTGCTGTTGGAGCGGCATTTGCTGGATTAAAGCCAATAGTCGAG
TTTATGACTTTTAATTTTTCTATGCAAGCTATTGACCAAATTGTGAATTCCGCAGCAAAAACAAATTATATGTCAGGCGG
ACAACTTGGATGCCCTATAGTATTTCGTGGACCAAATGGCGCTGCAGCAAGAGTTGCTGCACAACATTCTCAATGCTTTG
CAGCTTGGTATTCGCATATACCGGGGTTAAAAGTAATAGCACCCTATTTTGCCTCAGATTGCAGAGGTCTGCTTAAAGCT
GCAATTCGTGACCCTAATCCGGTAATATTTCTAGAAAACGAAATAGCTTATGGACATGAGCATGAAGTTTCTGACTCTGA
GCTATCAAACAAAGATTATCTACTTGAGATAGGCAAAGCTGCTGTTATACGGGAAGGAAAGGATGTAACTATCACTGCTT
TTTCATTAAAATTAATGGATGCCTTAAATGCAGCAGATTTACTTTCGAGTGAAGGTATAGAAGCTGAAGTTATTGACCTC
AGAACCTTAAGACCACTTGACACTCAAACTGTTATTAACTCTATTCAGAAGACTAATAGGTTAGTTAGTGTAGAAGAAGG
ATGGCCATTTGCAGGAATAGGAGCAGAGCTGTCAGCCGTTGTTATGGAACAAGGATTTGACTACCTTGATGCTCCAGTTG
TACGTGTAACTGGCAAGGACATCCCCTTACCTTACGCTGCAAATCTAGAAAAAAAAGCATTACCGCAAGTGGAAGATATA
GTTGAAGCCGTGCATCAGGTCTGTTTTAGAAAAAAATAG

Upstream 100 bases:

>100_bases
TGAGTTGGTCATGATGTTTGTAAAACTTAATTTGTACACTTTACTTACCCCCAGTAATTCTGATATAGTTACTTTAGTTA
AAGTCTATAAAAGTTTATAA

Downstream 100 bases:

>100_bases
TTCTTTTTTGTGTCTCCTGTTCCGCATTGGTCCATCAAAGAAGTATTTCCAGTGCTGCCTATACCACCCATTACTTTTAT
GACGATAGTTTTAGCACCGA

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MATLSVREALCTAIREEMQNDSDVLIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITEHGFAGLAVGAAFAGLKPIVE
FMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAARVAAQHSQCFAAWYSHIPGLKVIAPYFASDCRGLLKA
AIRDPNPVIFLENEIAYGHEHEVSDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
RTLRPLDTQTVINSIQKTNRLVSVEEGWPFAGIGAELSAVVMEQGFDYLDAPVVRVTGKDIPLPYAANLEKKALPQVEDI
VEAVHQVCFRKK

Sequences:

>Translated_332_residues
MATLSVREALCTAIREEMQNDSDVLIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITEHGFAGLAVGAAFAGLKPIVE
FMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAARVAAQHSQCFAAWYSHIPGLKVIAPYFASDCRGLLKA
AIRDPNPVIFLENEIAYGHEHEVSDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
RTLRPLDTQTVINSIQKTNRLVSVEEGWPFAGIGAELSAVVMEQGFDYLDAPVVRVTGKDIPLPYAANLEKKALPQVEDI
VEAVHQVCFRKK
>Mature_331_residues
ATLSVREALCTAIREEMQNDSDVLIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITEHGFAGLAVGAAFAGLKPIVEF
MTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNGAAARVAAQHSQCFAAWYSHIPGLKVIAPYFASDCRGLLKAA
IRDPNPVIFLENEIAYGHEHEVSDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDLR
TLRPLDTQTVINSIQKTNRLVSVEEGWPFAGIGAELSAVVMEQGFDYLDAPVVRVTGKDIPLPYAANLEKKALPQVEDIV
EAVHQVCFRKK

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=57.7160493827161, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=54.0123456790123, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI4557353, Length=304, Percent_Identity=35.1973684210526, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI34101272, Length=304, Percent_Identity=35.1973684210526, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI205277463, Length=292, Percent_Identity=28.4246575342466, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI4507521, Length=292, Percent_Identity=28.4246575342466, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI133778974, Length=93, Percent_Identity=40.8602150537634, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=62.2699386503067, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17506935, Length=325, Percent_Identity=36.3076923076923, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=60.0609756097561, Blast_Score=404, Evalue=1e-114,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=61.5384615384615, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=61.5384615384615, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=34.0490797546012, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=34.0490797546012, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24650943, Length=90, Percent_Identity=64.4444444444444, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24650945, Length=90, Percent_Identity=64.4444444444444, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45551847, Length=316, Percent_Identity=26.8987341772152, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45550715, Length=316, Percent_Identity=26.8987341772152, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24645119, Length=316, Percent_Identity=26.8987341772152, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36082; Mature: 35950

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLSVREALCTAIREEMQNDSDVLIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITE
CCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHHHHHHHHHCCCCEECCCCCC
HGFAGLAVGAAFAGLKPIVEFMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
AAARVAAQHSQCFAAWYSHIPGLKVIAPYFASDCRGLLKAAIRDPNPVIFLENEIAYGHE
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
HEVSDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
CCCCCHHCCCCCCEEECCCHHEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEH
RTLRPLDTQTVINSIQKTNRLVSVEEGWPFAGIGAELSAVVMEQGFDYLDAPVVRVTGKD
HCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCC
IPLPYAANLEKKALPQVEDIVEAVHQVCFRKK
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATLSVREALCTAIREEMQNDSDVLIMGEEVAEYDGAYKVTKGLLKEFGENRVVDTPITE
CCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHCCHHHHHHHHHHHHCCCCEECCCCCC
HGFAGLAVGAAFAGLKPIVEFMTFNFSMQAIDQIVNSAAKTNYMSGGQLGCPIVFRGPNG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
AAARVAAQHSQCFAAWYSHIPGLKVIAPYFASDCRGLLKAAIRDPNPVIFLENEIAYGHE
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
HEVSDSELSNKDYLLEIGKAAVIREGKDVTITAFSLKLMDALNAADLLSSEGIEAEVIDL
CCCCCHHCCCCCCEEECCCHHEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEH
RTLRPLDTQTVINSIQKTNRLVSVEEGWPFAGIGAELSAVVMEQGFDYLDAPVVRVTGKD
HCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCC
IPLPYAANLEKKALPQVEDIVEAVHQVCFRKK
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]