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The map label for this gene is sigW [H]

Identifier: 222527457

GI number: 222527457

Start: 5265148

End: 5265729

Strand: Reverse

Name: sigW [H]

Synonym: Chy400_4248

Alternate gene names: 222527457

Gene position: 5265729-5265148 (Counterclockwise)

Preceding gene: 222527459

Following gene: 222527456

Centisome position: 99.94

GC content: 54.98

Gene sequence:

>582_bases
GTGAGCGAATTACAGCCTGATGAAACAGAGCTTGTTGCTCGTGCCTGTGCAGGCGATCAATCGGCCTGTGCGACCATTGT
CCAGCGTTATACCGGTGTTCTGTATAATCAGGCATATCGTATGCTGGGTGACGCATTCGAGGCGGAAGATGCTGTTCAAG
AAGTATTTCTTCGGGCATTTCGTAATCTTCAGAGCTATGATCCGTCGCGCCGTTTGGTAACGTGGCTGCTGACGATTGGC
TCGAACTATTGTATTGATCGCCTGCGTCGCCGGCGCTTCAACTGGTTAACCCTTGAAGATGTGGCATTTTGGCTGCCGAG
CACGCAGCCGGGGCCGGAGCGAAGTGCATTGCAACGTGCGCAACAGGAGGTAGTGCAGCGGGCGCTACAGCGCCTCCCCG
TTACCTACCGTGGAGTCACCGTGTTGCGCTACTGGTATGATCTGTCGTATGAAGAGATCGGTCAGGTGTTAGGCTTGACC
GAAGCTACTGTGAAGACCCGGCTGCACCGGGCGCGCAAGATGTTGCAAGAGGCACTCGCTGCCGAGGAGGAGGTATGGAA
TATAGAGGGGATGATGAGTTGA

Upstream 100 bases:

>100_bases
TTGTGATCGCAGGTGACGTTGGATGGTTAGATTGCAGGAAGTTTTCGTGCGTTGCAACGTTTAATTGAATATAGCGTATA
ACATCATGTGAGGAATACCT

Downstream 100 bases:

>100_bases
GCGACACTGATCTGGCAGCAGCCGAAGCCGAACTACGCATGTTGTTAGCGCGCTGGGGAGAGCCGCAGATGGCAGCCCCG
CCGGCTGATTTGTCGGCGCG

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSELQPDETELVARACAGDQSACATIVQRYTGVLYNQAYRMLGDAFEAEDAVQEVFLRAFRNLQSYDPSRRLVTWLLTIG
SNYCIDRLRRRRFNWLTLEDVAFWLPSTQPGPERSALQRAQQEVVQRALQRLPVTYRGVTVLRYWYDLSYEEIGQVLGLT
EATVKTRLHRARKMLQEALAAEEEVWNIEGMMS

Sequences:

>Translated_193_residues
MSELQPDETELVARACAGDQSACATIVQRYTGVLYNQAYRMLGDAFEAEDAVQEVFLRAFRNLQSYDPSRRLVTWLLTIG
SNYCIDRLRRRRFNWLTLEDVAFWLPSTQPGPERSALQRAQQEVVQRALQRLPVTYRGVTVLRYWYDLSYEEIGQVLGLT
EATVKTRLHRARKMLQEALAAEEEVWNIEGMMS
>Mature_192_residues
SELQPDETELVARACAGDQSACATIVQRYTGVLYNQAYRMLGDAFEAEDAVQEVFLRAFRNLQSYDPSRRLVTWLLTIGS
NYCIDRLRRRRFNWLTLEDVAFWLPSTQPGPERSALQRAQQEVVQRALQRLPVTYRGVTVLRYWYDLSYEEIGQVLGLTE
ATVKTRLHRARKMLQEALAAEEEVWNIEGMMS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=187, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22372; Mature: 22241

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELQPDETELVARACAGDQSACATIVQRYTGVLYNQAYRMLGDAFEAEDAVQEVFLRAF
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQSYDPSRRLVTWLLTIGSNYCIDRLRRRRFNWLTLEDVAFWLPSTQPGPERSALQRA
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHH
QQEVVQRALQRLPVTYRGVTVLRYWYDLSYEEIGQVLGLTEATVKTRLHRARKMLQEALA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEEEVWNIEGMMS
HHHHHHHHHCCCC
>Mature Secondary Structure 
SELQPDETELVARACAGDQSACATIVQRYTGVLYNQAYRMLGDAFEAEDAVQEVFLRAF
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQSYDPSRRLVTWLLTIGSNYCIDRLRRRRFNWLTLEDVAFWLPSTQPGPERSALQRA
HHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHH
QQEVVQRALQRLPVTYRGVTVLRYWYDLSYEEIGQVLGLTEATVKTRLHRARKMLQEALA
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEEEVWNIEGMMS
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]