Click here to switch to the map view.

The map label for this gene is ykoH [H]

Identifier: 222527409

GI number: 222527409

Start: 5205822

End: 5207288

Strand: Reverse

Name: ykoH [H]

Synonym: Chy400_4200

Alternate gene names: 222527409

Gene position: 5207288-5205822 (Counterclockwise)

Preceding gene: 222527411

Following gene: 222527408

Centisome position: 98.83

GC content: 54.53

Gene sequence:

>1467_bases
TTGCCGCCGCTGCCATTTCGAGTGCGGCTGACGTTGTTGTACGCTGGTATGTTTGCTCTGGCACTCTGCGGAATTGGAGT
TGGTGTCCATTTCGCGGTTTCCCAGGCCCTGTATGCCGGTGTGCAGCGTGATCTTACGTCGGCGTCCCAGCAGGTAGAGG
CGATTCTTTCGGCAGTCGGGTTTCAGAGTATTCGTACTGCCAGTGGTGAATTGCAACTCTATCTGAATCGCGATTTTATC
AAGCTGTTTACCAACCGTGGGGTTGGCGTTCAGTTTTTTCGGATTGACGGCACCCTGGCGAGTAGTACACCGAATCTTGA
GTCGCACATGCTACCACTGCCGCCGGAGGCGATGCAATACATTAGCGCCGGCAATGATCAGATGAGTGTGGTGCGCGAAA
TTAACGGTGTGCCGGTCAAATCGCTGATCACACCGGTCACCCTGCGCAATGGTCAATTAATTGGCTTTTTGCAGGTCTCG
CGTCCACTTGATGATGTTGTGAACACCCTGACGTTGTTGCAGCAGATACTGATTGGCGGCGGTCTTCTGGCATTGATCAT
CAATGGTTTAGGCGCGATGTACCTCTCGAAGCGGGCGTTGCGCCCAATTGAGCAGATCACTATGACGGCGCAAAGTATTG
TGCGAGCCGAGGATTTGGGGCGGCGCATCCCCGAACCTGCGCAGCGCGATGAGTTGCAACTGCTGACGGTTACCATTAAC
GAACTTCTGTCACGCCTTGAGCGTCTCTTCGTGACGCAGCAGCGGTTTGTTGCCGATGTTAGCCACGAGTTGCGCACGCC
ATTGACGGCGATGCAGGGAAATCTCGAAATTCTGGATCGCGGTACCTGTCCCGATCCTGCGCTGCTCAAAGAGGTGATCG
GTGACATGCGGCGTGAGACGGCGCGTTTGATTCGGATGGTGAACGACCTGCTCTTGCTGGCGCAAAGCGAAACTCACGTG
GTGTTGCGCCATGAACCGGTAGAACTCGATACACTCTTGTTAGAGGTCTTCCGCGAGTTACGACCACTGGCCGGACAGGT
ACGCCTGCGGATCGGTGCTGAAGATCAAATCCTGGTGTACGGTGACCGTGACCGTATCAAACAGGCGTTGCTGAATTTGG
GGGTTAATGCGTTGCAGTATACGCCACCCGGTGGACTGGTCATTCTGAGTTTAGAACGTTACGAGCAGTTTGCTTGTCTG
AGCGTTGCCGATACAGGTGTTGGCATCAGTGAAGAGGCACGTGCGCATATCTTTGAGCGGTTTTACCGTGTGGATCGCTC
GCGATCACGGCATAGTGGTGGGGCCGGTTTAGGACTATCGATAGTGAAATGGGTTGCCGAGGCGCATGGCGGCTATGTGA
CTGTGGCGAGTGAGGTTGGGCGGGGAAGTACATTCGCAATATACCTGCCGTTGCCGGAAGCAAATCCTGTCCCCGTTACT
GAGGTTGAAGCGGGTAATACCAAATAA

Upstream 100 bases:

>100_bases
TGAAGCAGTAACAGATCAGGTAACGTCGGTAGACCAGTCGCAGCCGACTGCTGAAGGACTGGAACAACGACGCTCTCTCT
TTAATCGTATAGGACTGCAT

Downstream 100 bases:

>100_bases
TGGTAATCAGGTCGCGATAGTCTGTTGTGCGGTATGCATGACCTTTACGGTGGGAGTGCGTTGGCAACCATCCGGTTTCT
GACCACGTCCAGAGCCAGAT

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MPPLPFRVRLTLLYAGMFALALCGIGVGVHFAVSQALYAGVQRDLTSASQQVEAILSAVGFQSIRTASGELQLYLNRDFI
KLFTNRGVGVQFFRIDGTLASSTPNLESHMLPLPPEAMQYISAGNDQMSVVREINGVPVKSLITPVTLRNGQLIGFLQVS
RPLDDVVNTLTLLQQILIGGGLLALIINGLGAMYLSKRALRPIEQITMTAQSIVRAEDLGRRIPEPAQRDELQLLTVTIN
ELLSRLERLFVTQQRFVADVSHELRTPLTAMQGNLEILDRGTCPDPALLKEVIGDMRRETARLIRMVNDLLLLAQSETHV
VLRHEPVELDTLLLEVFRELRPLAGQVRLRIGAEDQILVYGDRDRIKQALLNLGVNALQYTPPGGLVILSLERYEQFACL
SVADTGVGISEEARAHIFERFYRVDRSRSRHSGGAGLGLSIVKWVAEAHGGYVTVASEVGRGSTFAIYLPLPEANPVPVT
EVEAGNTK

Sequences:

>Translated_488_residues
MPPLPFRVRLTLLYAGMFALALCGIGVGVHFAVSQALYAGVQRDLTSASQQVEAILSAVGFQSIRTASGELQLYLNRDFI
KLFTNRGVGVQFFRIDGTLASSTPNLESHMLPLPPEAMQYISAGNDQMSVVREINGVPVKSLITPVTLRNGQLIGFLQVS
RPLDDVVNTLTLLQQILIGGGLLALIINGLGAMYLSKRALRPIEQITMTAQSIVRAEDLGRRIPEPAQRDELQLLTVTIN
ELLSRLERLFVTQQRFVADVSHELRTPLTAMQGNLEILDRGTCPDPALLKEVIGDMRRETARLIRMVNDLLLLAQSETHV
VLRHEPVELDTLLLEVFRELRPLAGQVRLRIGAEDQILVYGDRDRIKQALLNLGVNALQYTPPGGLVILSLERYEQFACL
SVADTGVGISEEARAHIFERFYRVDRSRSRHSGGAGLGLSIVKWVAEAHGGYVTVASEVGRGSTFAIYLPLPEANPVPVT
EVEAGNTK
>Mature_487_residues
PPLPFRVRLTLLYAGMFALALCGIGVGVHFAVSQALYAGVQRDLTSASQQVEAILSAVGFQSIRTASGELQLYLNRDFIK
LFTNRGVGVQFFRIDGTLASSTPNLESHMLPLPPEAMQYISAGNDQMSVVREINGVPVKSLITPVTLRNGQLIGFLQVSR
PLDDVVNTLTLLQQILIGGGLLALIINGLGAMYLSKRALRPIEQITMTAQSIVRAEDLGRRIPEPAQRDELQLLTVTINE
LLSRLERLFVTQQRFVADVSHELRTPLTAMQGNLEILDRGTCPDPALLKEVIGDMRRETARLIRMVNDLLLLAQSETHVV
LRHEPVELDTLLLEVFRELRPLAGQVRLRIGAEDQILVYGDRDRIKQALLNLGVNALQYTPPGGLVILSLERYEQFACLS
VADTGVGISEEARAHIFERFYRVDRSRSRHSGGAGLGLSIVKWVAEAHGGYVTVASEVGRGSTFAIYLPLPEANPVPVTE
VEAGNTK

Specific function: Probable member of the two-component regulatory system ykoH/ykoG. Potentially phosphorylates ykoG [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=277, Percent_Identity=36.101083032491, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1786783, Length=368, Percent_Identity=30.4347826086957, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786600, Length=250, Percent_Identity=34.4, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1790346, Length=238, Percent_Identity=32.7731092436975, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=31.3559322033898, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788279, Length=418, Percent_Identity=25.3588516746411, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=28.9256198347107, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789149, Length=372, Percent_Identity=26.6129032258064, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081816, Length=268, Percent_Identity=32.8358208955224, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790861, Length=311, Percent_Identity=29.903536977492, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787894, Length=248, Percent_Identity=32.6612903225806, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=30.2904564315353, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789403, Length=274, Percent_Identity=29.9270072992701, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=31.0924369747899, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790551, Length=225, Percent_Identity=31.5555555555556, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788713, Length=265, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786912, Length=245, Percent_Identity=27.3469387755102, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789808, Length=311, Percent_Identity=27.3311897106109, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082128, Length=252, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=235, Percent_Identity=31.4893617021277, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53608; Mature: 53477

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLPFRVRLTLLYAGMFALALCGIGVGVHFAVSQALYAGVQRDLTSASQQVEAILSAVG
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSIRTASGELQLYLNRDFIKLFTNRGVGVQFFRIDGTLASSTPNLESHMLPLPPEAMQY
HHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEEEECCEEECCCCCHHHCCCCCCHHHHHH
ISAGNDQMSVVREINGVPVKSLITPVTLRNGQLIGFLQVSRPLDDVVNTLTLLQQILIGG
HHCCCHHHHHHHHCCCCCHHHHHCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
GLLALIINGLGAMYLSKRALRPIEQITMTAQSIVRAEDLGRRIPEPAQRDELQLLTVTIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
ELLSRLERLFVTQQRFVADVSHELRTPLTAMQGNLEILDRGTCPDPALLKEVIGDMRRET
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
ARLIRMVNDLLLLAQSETHVVLRHEPVELDTLLLEVFRELRPLAGQVRLRIGAEDQILVY
HHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEE
GDRDRIKQALLNLGVNALQYTPPGGLVILSLERYEQFACLSVADTGVGISEEARAHIFER
CCHHHHHHHHHHCCCCCEEECCCCCEEEEEHHHHHHHHEEEECCCCCCCCHHHHHHHHHH
FYRVDRSRSRHSGGAGLGLSIVKWVAEAHGGYVTVASEVGRGSTFAIYLPLPEANPVPVT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCCE
EVEAGNTK
ECCCCCCC
>Mature Secondary Structure 
PPLPFRVRLTLLYAGMFALALCGIGVGVHFAVSQALYAGVQRDLTSASQQVEAILSAVG
CCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSIRTASGELQLYLNRDFIKLFTNRGVGVQFFRIDGTLASSTPNLESHMLPLPPEAMQY
HHHHHCCCCCEEEEECCHHHHHHHCCCCCEEEEEECCEEECCCCCHHHCCCCCCHHHHHH
ISAGNDQMSVVREINGVPVKSLITPVTLRNGQLIGFLQVSRPLDDVVNTLTLLQQILIGG
HHCCCHHHHHHHHCCCCCHHHHHCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
GLLALIINGLGAMYLSKRALRPIEQITMTAQSIVRAEDLGRRIPEPAQRDELQLLTVTIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
ELLSRLERLFVTQQRFVADVSHELRTPLTAMQGNLEILDRGTCPDPALLKEVIGDMRRET
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
ARLIRMVNDLLLLAQSETHVVLRHEPVELDTLLLEVFRELRPLAGQVRLRIGAEDQILVY
HHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEE
GDRDRIKQALLNLGVNALQYTPPGGLVILSLERYEQFACLSVADTGVGISEEARAHIFER
CCHHHHHHHHHHCCCCCEEECCCCCEEEEEHHHHHHHHEEEECCCCCCCCHHHHHHHHHH
FYRVDRSRSRHSGGAGLGLSIVKWVAEAHGGYVTVASEVGRGSTFAIYLPLPEANPVPVT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCCE
EVEAGNTK
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]