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The map label for this gene is mdeA [H]

Identifier: 222526962

GI number: 222526962

Start: 4661859

End: 4663154

Strand: Direct

Name: mdeA [H]

Synonym: Chy400_3740

Alternate gene names: 222526962

Gene position: 4661859-4663154 (Clockwise)

Preceding gene: 222526957

Following gene: 222526963

Centisome position: 88.48

GC content: 56.33

Gene sequence:

>1296_bases
ATGTCTGATGCTCCGCGCTTCACCGGTTTTGAAACTCTCGCCCTGCATGCCGGCCAAACCCCCGATCCGACCACTGGGTC
GCGAGCTGTTCCTATCTACGCCACCACATCCTATCAATTCAAAGACACAGACCATGCAGCACGCCTGTTCAATCTGCAAG
AGTTTGGTAATATCTACACTCGCATTATGAATCCGACCACCGATGTCTTTGAACAGCGGATGGCGGCACTGGAGGGCGGA
GTAGGTGCGCTGGCGCTGGCATCGGGGCAGGCCGCTGAAACGCTGGCTATTCTCAACCTGGCCGGAAGTGGCGACAATAT
CGTCGCATCCTCTGACCTGTATGGCGGCACCTACAACCTCTTCCGTCATACATTACCAAAGTTAGGCATTACGACTCGCT
TTGTTGATGCCCGTGATTACGATGGCTTTGCCGCTGCGATTGATGGGCGTACCAAAGCCTTCTTCCTCGAATTAGTCGGC
AATCCACGGCTTGATGTGCTCGATCTGGAGCGCATTGCCGCAATTGCGCATGCGCAGGGTGTGCCGGTCATCGTCGATGC
GACCACGGTCACACCGTACCTGTGGCAACCGATCCAGCACGGCGCCGATATTGTGATTCACTCGGCAACCAAGTACATTG
GTGGTCATGGCACGGCTATCGGAGGCATCATTGTTGACAGTGGTAAGTTTGATTGGGCAGCCAGTGGCCGTTTTCCCGAA
TTTACCAATCCCGATCCGAGCTATCACGGCCTGATCTATACCCAGGCTTTCGGCAACCTGGCGTACATTATCAAGGCACG
AGTGCAAGGGCTACGCGACATTGGGGCTGCGCTTAGCCCATTTAACAGCTTCCTGTTCCTGCAAGGGCTGGAGACGTTGC
CACTCCGGATGGAACGACACAGCAAGAATGCGCTGGCAGTGGCCCGTTATCTGAGTGAGCATCCCAAAGTGGCGTGGGTC
AACTACCCCGGTCTTCCCAGCCATCCAAGCTATGCCCTGGCTCAGAAATATCTGCCGCGTGGGCAGAGTGGGATTGTTGG
CTTCGGCCTGAAGGGTGGGCGTGCCGCCGGACGTACCTTCATTGAGCGGTTACGGCTCTTCTCGCACCTCGCCAATATTG
GCGATGCCAAGAGCCTTGCGATCCATCCGGCAACCACCACCCACAGCCAGTTGACGCCGGAAGAACAATTGCTGACCGGC
GTGACCGACGATTATGTCCGGCTCTCGATTGGCCTGGAGACGATTGACGATATTCTGGCCGATCTTGATCATGCCCTGGC
AGGAACCCCATCATAG

Upstream 100 bases:

>100_bases
CGTGGAAGAACGGCTTAGTGCAACGATTTCACGCCCTCATCGCACGTTGAGGGCGTTTTTGTTGCCTGCAATCTGGCTTA
TCTGTTTTGAGGAGGTTGTT

Downstream 100 bases:

>100_bases
TGTTGGATCAGAGAGCAACGACAACGGCGTCGCTGCTCTCTGATACCGGCAAGGAGCAACGACAATATGGAAGCAATTGT
GCAGGCGCCAACCCCAGAGG

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSDAPRFTGFETLALHAGQTPDPTTGSRAVPIYATTSYQFKDTDHAARLFNLQEFGNIYTRIMNPTTDVFEQRMAALEGG
VGALALASGQAAETLAILNLAGSGDNIVASSDLYGGTYNLFRHTLPKLGITTRFVDARDYDGFAAAIDGRTKAFFLELVG
NPRLDVLDLERIAAIAHAQGVPVIVDATTVTPYLWQPIQHGADIVIHSATKYIGGHGTAIGGIIVDSGKFDWAASGRFPE
FTNPDPSYHGLIYTQAFGNLAYIIKARVQGLRDIGAALSPFNSFLFLQGLETLPLRMERHSKNALAVARYLSEHPKVAWV
NYPGLPSHPSYALAQKYLPRGQSGIVGFGLKGGRAAGRTFIERLRLFSHLANIGDAKSLAIHPATTTHSQLTPEEQLLTG
VTDDYVRLSIGLETIDDILADLDHALAGTPS

Sequences:

>Translated_431_residues
MSDAPRFTGFETLALHAGQTPDPTTGSRAVPIYATTSYQFKDTDHAARLFNLQEFGNIYTRIMNPTTDVFEQRMAALEGG
VGALALASGQAAETLAILNLAGSGDNIVASSDLYGGTYNLFRHTLPKLGITTRFVDARDYDGFAAAIDGRTKAFFLELVG
NPRLDVLDLERIAAIAHAQGVPVIVDATTVTPYLWQPIQHGADIVIHSATKYIGGHGTAIGGIIVDSGKFDWAASGRFPE
FTNPDPSYHGLIYTQAFGNLAYIIKARVQGLRDIGAALSPFNSFLFLQGLETLPLRMERHSKNALAVARYLSEHPKVAWV
NYPGLPSHPSYALAQKYLPRGQSGIVGFGLKGGRAAGRTFIERLRLFSHLANIGDAKSLAIHPATTTHSQLTPEEQLLTG
VTDDYVRLSIGLETIDDILADLDHALAGTPS
>Mature_430_residues
SDAPRFTGFETLALHAGQTPDPTTGSRAVPIYATTSYQFKDTDHAARLFNLQEFGNIYTRIMNPTTDVFEQRMAALEGGV
GALALASGQAAETLAILNLAGSGDNIVASSDLYGGTYNLFRHTLPKLGITTRFVDARDYDGFAAAIDGRTKAFFLELVGN
PRLDVLDLERIAAIAHAQGVPVIVDATTVTPYLWQPIQHGADIVIHSATKYIGGHGTAIGGIIVDSGKFDWAASGRFPEF
TNPDPSYHGLIYTQAFGNLAYIIKARVQGLRDIGAALSPFNSFLFLQGLETLPLRMERHSKNALAVARYLSEHPKVAWVN
YPGLPSHPSYALAQKYLPRGQSGIVGFGLKGGRAAGRTFIERLRLFSHLANIGDAKSLAIHPATTTHSQLTPEEQLLTGV
TDDYVRLSIGLETIDDILADLDHALAGTPS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=410, Percent_Identity=35.1219512195122, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI299473759, Length=410, Percent_Identity=30.9756097560976, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI25453487, Length=409, Percent_Identity=31.5403422982885, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1790375, Length=413, Percent_Identity=30.7506053268765, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789383, Length=426, Percent_Identity=26.9953051643192, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538055, Length=411, Percent_Identity=35.2798053527981, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17560056, Length=411, Percent_Identity=35.036496350365, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71980708, Length=404, Percent_Identity=32.1782178217822, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=52.4361948955916, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=33.806146572104, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=441, Percent_Identity=25.6235827664399, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=26.5536723163842, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=428, Percent_Identity=24.5327102803738, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=35.1415094339623, Blast_Score=234, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46433; Mature: 46302

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAPRFTGFETLALHAGQTPDPTTGSRAVPIYATTSYQFKDTDHAARLFNLQEFGNIYT
CCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHH
RIMNPTTDVFEQRMAALEGGVGALALASGQAAETLAILNLAGSGDNIVASSDLYGGTYNL
HHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCEEEECCCCCCHHHH
FRHTLPKLGITTRFVDARDYDGFAAAIDGRTKAFFLELVGNPRLDVLDLERIAAIAHAQG
HHHHHHCCCCEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCEEHHHHHHHHHHCCC
VPVIVDATTVTPYLWQPIQHGADIVIHSATKYIGGHGTAIGGIIVDSGKFDWAASGRFPE
CCEEEECCCCCHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEEEEECCCCCEEECCCCCC
FTNPDPSYHGLIYTQAFGNLAYIIKARVQGLRDIGAALSPFNSFLFLQGLETLPLRMERH
CCCCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
SKNALAVARYLSEHPKVAWVNYPGLPSHPSYALAQKYLPRGQSGIVGFGLKGGRAAGRTF
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHH
IERLRLFSHLANIGDAKSLAIHPATTTHSQLTPEEQLLTGVTDDYVRLSIGLETIDDILA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEECHHHHHHHHH
DLDHALAGTPS
HHHHHHCCCCC
>Mature Secondary Structure 
SDAPRFTGFETLALHAGQTPDPTTGSRAVPIYATTSYQFKDTDHAARLFNLQEFGNIYT
CCCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCEEECCCHHHHHHHHHHHHHHHHH
RIMNPTTDVFEQRMAALEGGVGALALASGQAAETLAILNLAGSGDNIVASSDLYGGTYNL
HHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCEEEECCCCCCHHHH
FRHTLPKLGITTRFVDARDYDGFAAAIDGRTKAFFLELVGNPRLDVLDLERIAAIAHAQG
HHHHHHCCCCEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCEEHHHHHHHHHHCCC
VPVIVDATTVTPYLWQPIQHGADIVIHSATKYIGGHGTAIGGIIVDSGKFDWAASGRFPE
CCEEEECCCCCHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEEEEECCCCCEEECCCCCC
FTNPDPSYHGLIYTQAFGNLAYIIKARVQGLRDIGAALSPFNSFLFLQGLETLPLRMERH
CCCCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHC
SKNALAVARYLSEHPKVAWVNYPGLPSHPSYALAQKYLPRGQSGIVGFGLKGGRAAGRTF
CCHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCHHHHHH
IERLRLFSHLANIGDAKSLAIHPATTTHSQLTPEEQLLTGVTDDYVRLSIGLETIDDILA
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCEEEEEECHHHHHHHHH
DLDHALAGTPS
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]