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The map label for this gene is dapE [H]

Identifier: 222526815

GI number: 222526815

Start: 4464115

End: 4465203

Strand: Reverse

Name: dapE [H]

Synonym: Chy400_3590

Alternate gene names: 222526815

Gene position: 4465203-4464115 (Counterclockwise)

Preceding gene: 222526816

Following gene: 222526814

Centisome position: 84.75

GC content: 58.49

Gene sequence:

>1089_bases
ATGACTGAACTTCGTGATGAATTGATTCGTCTCACCTGCGACCTGATCCGTTTTGAGACGATTGCCGACCGTCCCGATCA
GTTACAGGCGGCGATAGATTATGTCGCCGATTATCTTGCCGACGTTCCGGGCATCCAGATCGAAAAGAGCTGTGTAGCCG
ACAAGCCGGCGCTGGTCGTTACGCTGCAACCCACGCGCTCGCCGCGTCTGATGCTCAACGGACATCTGGATGTCGTGGTT
GGACAGCCGGCACAGTTCGTACCAGAGGTGCGTGATGGCCGCATCTATGGCCGTGGCAGTCAGGATATGAAGGGCAGCAT
TGCCGTGATGATGCGCCTGATCCGCGATCTGGCTCAACGGCCCAATCCGCCGGATGTCGGTTTTCAATTCGTCACCGACG
AAGAGATTGGTGGACGCCACGGCACCGGTCGGTTGCGCGATGAAGGATGGCACTGCGACTTTATGCTCTGCCTGGAACCA
ACCGACCTGGGGATTATGTTCGAGCACAAAGGCGGGATGTGGGCACAGTTGCGCATTCCCGGTCGAGCTGCTCATGGATC
ACGGCCCTGGGAAGGCGATAACCCTGTCTACCGACTGATGCAGGGCATTCAAGCGATAAGCGAGCGCTACCCACCACCAA
CTGGCCCTCACGATTGGCGAACGTCGGTAACACCAACCGAGATTCGCATGGGGGCAGGTTCGCGGAATCAGGTGCCGGCA
GAGGCGCTGGTTACGTTTGATATTCGCTGGACGGCTGACACCACACCGGAGACGATTCAAGCCGATCTGGCGGCAGCGTT
TCCCGACGCCGAATTCATCAGTGTCATGGCCAGTGCCGGCCTGCGCACCGATCCCGAACACCAGGAAGTCGGGCGCATTG
CCGACATCGTCGAACGGCATATCGGCCACACCCCCCGTTTCTACCGCGAACATTTTGCGACCGATGCCCGTTACTATTCG
CACATTGGCATCCCTGCCATCTGCCTCGGCCCGGTTGGGGCCGGCCTGCATTCGGCAGAGGAGTGGGTAGAGATTGCCAG
TCTGACGAGCCTGTACCAGATTATTCTCGATTATATCGACACCATGTAG

Upstream 100 bases:

>100_bases
GTCTCTCTCGCCAGACTCGCGATGTTTCACAACGTGGTACACTAGGAAGCAGTTAGTAGCGATTGCCTGTAGCAATCGCC
ATCACACCGAGAGCAAGCGT

Downstream 100 bases:

>100_bases
GATGACGACCTTCATATGCTCTACGTATTACAAACCATTCCCGGCCTGGCTGCGCTGACCTGGCGCGAAGTCGAACAGAC
CATTCGCCCGGAGACAGACC

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTELRDELIRLTCDLIRFETIADRPDQLQAAIDYVADYLADVPGIQIEKSCVADKPALVVTLQPTRSPRLMLNGHLDVVV
GQPAQFVPEVRDGRIYGRGSQDMKGSIAVMMRLIRDLAQRPNPPDVGFQFVTDEEIGGRHGTGRLRDEGWHCDFMLCLEP
TDLGIMFEHKGGMWAQLRIPGRAAHGSRPWEGDNPVYRLMQGIQAISERYPPPTGPHDWRTSVTPTEIRMGAGSRNQVPA
EALVTFDIRWTADTTPETIQADLAAAFPDAEFISVMASAGLRTDPEHQEVGRIADIVERHIGHTPRFYREHFATDARYYS
HIGIPAICLGPVGAGLHSAEEWVEIASLTSLYQIILDYIDTM

Sequences:

>Translated_362_residues
MTELRDELIRLTCDLIRFETIADRPDQLQAAIDYVADYLADVPGIQIEKSCVADKPALVVTLQPTRSPRLMLNGHLDVVV
GQPAQFVPEVRDGRIYGRGSQDMKGSIAVMMRLIRDLAQRPNPPDVGFQFVTDEEIGGRHGTGRLRDEGWHCDFMLCLEP
TDLGIMFEHKGGMWAQLRIPGRAAHGSRPWEGDNPVYRLMQGIQAISERYPPPTGPHDWRTSVTPTEIRMGAGSRNQVPA
EALVTFDIRWTADTTPETIQADLAAAFPDAEFISVMASAGLRTDPEHQEVGRIADIVERHIGHTPRFYREHFATDARYYS
HIGIPAICLGPVGAGLHSAEEWVEIASLTSLYQIILDYIDTM
>Mature_361_residues
TELRDELIRLTCDLIRFETIADRPDQLQAAIDYVADYLADVPGIQIEKSCVADKPALVVTLQPTRSPRLMLNGHLDVVVG
QPAQFVPEVRDGRIYGRGSQDMKGSIAVMMRLIRDLAQRPNPPDVGFQFVTDEEIGGRHGTGRLRDEGWHCDFMLCLEPT
DLGIMFEHKGGMWAQLRIPGRAAHGSRPWEGDNPVYRLMQGIQAISERYPPPTGPHDWRTSVTPTEIRMGAGSRNQVPAE
ALVTFDIRWTADTTPETIQADLAAAFPDAEFISVMASAGLRTDPEHQEVGRIADIVERHIGHTPRFYREHFATDARYYSH
IGIPAICLGPVGAGLHSAEEWVEIASLTSLYQIILDYIDTM

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=324, Percent_Identity=29.320987654321, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40378; Mature: 40247

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELRDELIRLTCDLIRFETIADRPDQLQAAIDYVADYLADVPGIQIEKSCVADKPALVV
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCEEEE
TLQPTRSPRLMLNGHLDVVVGQPAQFVPEVRDGRIYGRGSQDMKGSIAVMMRLIRDLAQR
EECCCCCCEEEEECCEEEEECCCHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHC
PNPPDVGFQFVTDEEIGGRHGTGRLRDEGWHCDFMLCLEPTDLGIMFEHKGGMWAQLRIP
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECC
GRAAHGSRPWEGDNPVYRLMQGIQAISERYPPPTGPHDWRTSVTPTEIRMGAGSRNQVPA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEECCCCCCCCCC
EALVTFDIRWTADTTPETIQADLAAAFPDAEFISVMASAGLRTDPEHQEVGRIADIVERH
EEEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IGHTPRFYREHFATDARYYSHIGIPAICLGPVGAGLHSAEEWVEIASLTSLYQIILDYID
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TM
CC
>Mature Secondary Structure 
TELRDELIRLTCDLIRFETIADRPDQLQAAIDYVADYLADVPGIQIEKSCVADKPALVV
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCEEEE
TLQPTRSPRLMLNGHLDVVVGQPAQFVPEVRDGRIYGRGSQDMKGSIAVMMRLIRDLAQR
EECCCCCCEEEEECCEEEEECCCHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHC
PNPPDVGFQFVTDEEIGGRHGTGRLRDEGWHCDFMLCLEPTDLGIMFEHKGGMWAQLRIP
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECC
GRAAHGSRPWEGDNPVYRLMQGIQAISERYPPPTGPHDWRTSVTPTEIRMGAGSRNQVPA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEECCCCCCCCCC
EALVTFDIRWTADTTPETIQADLAAAFPDAEFISVMASAGLRTDPEHQEVGRIADIVERH
EEEEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IGHTPRFYREHFATDARYYSHIGIPAICLGPVGAGLHSAEEWVEIASLTSLYQIILDYID
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TM
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA