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The map label for this gene is mdeA [H]

Identifier: 222526759

GI number: 222526759

Start: 4399921

End: 4401189

Strand: Direct

Name: mdeA [H]

Synonym: Chy400_3533

Alternate gene names: 222526759

Gene position: 4399921-4401189 (Clockwise)

Preceding gene: 222526758

Following gene: 222526760

Centisome position: 83.51

GC content: 56.74

Gene sequence:

>1269_bases
ATGACGAAGCAGAGTAAACGAACGACCATCGACGGCCATCGTCTGCATCCCGAAAGTCTGATGATGAGTTATGGGTATGT
GCCGGCACTCTCTGAAGGTGCGATCAAGTGTCCGATTTTTCAGACCTCAACCTTCGTGTTTCAGACCGCTGAAGAGGGTA
AAGCCTTCTTCGAGCTGGCCTACGGTCTACGCCATCCCCGTGAGGGCGAAGAGATTGGCCTGATCTACAGCCGGCTTAAC
AACCCCGATCTTGAAATTCTGGAAGACCGGCTTACGCTGTGGGATCAGGCTGAGGCCGCAGCAGTGTTTGCCAGCGGGAT
GGCGGCTATCACCACCACCCTGCTCACCTTCTTACGGCCCGGCGATGTCATTGTGCATAGTGAACCGGTCTATGGCGGTA
CTGATTACCTGTTGAAGCACGTCTTACCGTCATTGGGGATACGACGATTTGGTTTTATGTCGGGTGTCTCACCCGAACAG
GTTGAAGTTCAACTGCGACGAGCCGGCCTGTTCGATGCGGTAGGGATGATCTATCTTGAAACGCCGGCGAATCCAACAAA
TGCGCTGGTAGACATTGCCGGCTTTGCAGCGCTGGCGAAGCGGATGCCACGACGAGTCTTAACCGCAGTTGATAATACAT
TTCTCGGCCCGCTCTGGCAACATCCCCTGGCCCATGGTGCCGATCTGGTGCTCTACTCGGCCACCAAGTATATCGGTGGC
CACAGTGATGTGATTGCCGGGGTCTGTCTGGGCAGTCGTGAGTTGCTGGCCCAGGTGAAGGGGATGCGCACCATTATGGG
AACGATGGCGGGTGCGCATACCGGCTGGCTCCTGCTACGGTCGCTGGAGACGCTCAAGATTCGGATGGAGACGCAGCAAG
CCGGCGCACGTCGGGTAGCCGATTTTCTGGCAACCCATCCCAAAGTGGCGCGGGTCTACTATCTCGGCCATCTTGAGAGT
AATGACCCCCAATACCACATCTATCGGCGGCAATGTTTGGGACCGGGGGCAATGATCTCGTTTGATCTGCACGGTGGTGA
GGCGGAAGCGTTTCGCTTTCTCAACCATCTGCAATTGATCCACCTGGCAGTGAGTCTGGGTGGTACCGAGTCGCTGGCCG
AGCACCCGGCTTCGATGACCCACTCGGATGTCGATCCCGAAGAGCGCATCGAGTTTGGGATTGGCCCGGCCATGATCCGG
CTGTCGGTAGGAGTGGAGCATCCTGATGATCTGATCGCCGATCTGAATCAGGCGTTGCGGGCGGTATAA

Upstream 100 bases:

>100_bases
TGGTACCATTGTAGGTGGCCTGGTTGGCCTGATTATTTACAGTGTTTCTCTTCTCTTTTCCTGAGTGAGCGATGCTGCTC
GTGACGCGAGGAGGCGCATG

Downstream 100 bases:

>100_bases
TTTTCGAGAAGTTAGAAAATAGGAGATAGGAGTTAGAAAATAGGGGATAGGAAAGAGGGAGAGGAGGTCGGGTGTAGGGA
AGACATTCTCGAACCTTGGT

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTKQSKRTTIDGHRLHPESLMMSYGYVPALSEGAIKCPIFQTSTFVFQTAEEGKAFFELAYGLRHPREGEEIGLIYSRLN
NPDLEILEDRLTLWDQAEAAAVFASGMAAITTTLLTFLRPGDVIVHSEPVYGGTDYLLKHVLPSLGIRRFGFMSGVSPEQ
VEVQLRRAGLFDAVGMIYLETPANPTNALVDIAGFAALAKRMPRRVLTAVDNTFLGPLWQHPLAHGADLVLYSATKYIGG
HSDVIAGVCLGSRELLAQVKGMRTIMGTMAGAHTGWLLLRSLETLKIRMETQQAGARRVADFLATHPKVARVYYLGHLES
NDPQYHIYRRQCLGPGAMISFDLHGGEAEAFRFLNHLQLIHLAVSLGGTESLAEHPASMTHSDVDPEERIEFGIGPAMIR
LSVGVEHPDDLIADLNQALRAV

Sequences:

>Translated_422_residues
MTKQSKRTTIDGHRLHPESLMMSYGYVPALSEGAIKCPIFQTSTFVFQTAEEGKAFFELAYGLRHPREGEEIGLIYSRLN
NPDLEILEDRLTLWDQAEAAAVFASGMAAITTTLLTFLRPGDVIVHSEPVYGGTDYLLKHVLPSLGIRRFGFMSGVSPEQ
VEVQLRRAGLFDAVGMIYLETPANPTNALVDIAGFAALAKRMPRRVLTAVDNTFLGPLWQHPLAHGADLVLYSATKYIGG
HSDVIAGVCLGSRELLAQVKGMRTIMGTMAGAHTGWLLLRSLETLKIRMETQQAGARRVADFLATHPKVARVYYLGHLES
NDPQYHIYRRQCLGPGAMISFDLHGGEAEAFRFLNHLQLIHLAVSLGGTESLAEHPASMTHSDVDPEERIEFGIGPAMIR
LSVGVEHPDDLIADLNQALRAV
>Mature_421_residues
TKQSKRTTIDGHRLHPESLMMSYGYVPALSEGAIKCPIFQTSTFVFQTAEEGKAFFELAYGLRHPREGEEIGLIYSRLNN
PDLEILEDRLTLWDQAEAAAVFASGMAAITTTLLTFLRPGDVIVHSEPVYGGTDYLLKHVLPSLGIRRFGFMSGVSPEQV
EVQLRRAGLFDAVGMIYLETPANPTNALVDIAGFAALAKRMPRRVLTAVDNTFLGPLWQHPLAHGADLVLYSATKYIGGH
SDVIAGVCLGSRELLAQVKGMRTIMGTMAGAHTGWLLLRSLETLKIRMETQQAGARRVADFLATHPKVARVYYLGHLESN
DPQYHIYRRQCLGPGAMISFDLHGGEAEAFRFLNHLQLIHLAVSLGGTESLAEHPASMTHSDVDPEERIEFGIGPAMIRL
SVGVEHPDDLIADLNQALRAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=38.7362637362637, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI299473759, Length=261, Percent_Identity=41.7624521072797, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI25453487, Length=354, Percent_Identity=34.7457627118644, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1790375, Length=390, Percent_Identity=37.9487179487179, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1789383, Length=403, Percent_Identity=26.302729528536, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=366, Percent_Identity=37.9781420765027, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=34.9397590361446, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=358, Percent_Identity=37.1508379888268, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6319307, Length=352, Percent_Identity=36.6477272727273, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=28.4403669724771, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=355, Percent_Identity=32.112676056338, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=368, Percent_Identity=30.1630434782609, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=346, Percent_Identity=23.6994219653179, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922554, Length=365, Percent_Identity=38.0821917808219, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46352; Mature: 46221

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQSKRTTIDGHRLHPESLMMSYGYVPALSEGAIKCPIFQTSTFVFQTAEEGKAFFELA
CCCCCCCCCCCCCEECHHHHHHHCCCCCCCCCCCEECEEEECCEEEEEEHHHHHHHHHHH
YGLRHPREGEEIGLIYSRLNNPDLEILEDRLTLWDQAEAAAVFASGMAAITTTLLTFLRP
HHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
GDVIVHSEPVYGGTDYLLKHVLPSLGIRRFGFMSGVSPEQVEVQLRRAGLFDAVGMIYLE
CCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCHHHHCCEEEEE
TPANPTNALVDIAGFAALAKRMPRRVLTAVDNTFLGPLWQHPLAHGADLVLYSATKYIGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHCCCCEEEEEHHHHCCC
HSDVIAGVCLGSRELLAQVKGMRTIMGTMAGAHTGWLLLRSLETLKIRMETQQAGARRVA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHHHHHHHHH
DFLATHPKVARVYYLGHLESNDPQYHIYRRQCLGPGAMISFDLHGGEAEAFRFLNHLQLI
HHHHCCCCEEEEEEEEEECCCCCCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
HLAVSLGGTESLAEHPASMTHSDVDPEERIEFGIGPAMIRLSVGVEHPDDLIADLNQALR
HHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
AV
CC
>Mature Secondary Structure 
TKQSKRTTIDGHRLHPESLMMSYGYVPALSEGAIKCPIFQTSTFVFQTAEEGKAFFELA
CCCCCCCCCCCCEECHHHHHHHCCCCCCCCCCCEECEEEECCEEEEEEHHHHHHHHHHH
YGLRHPREGEEIGLIYSRLNNPDLEILEDRLTLWDQAEAAAVFASGMAAITTTLLTFLRP
HHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
GDVIVHSEPVYGGTDYLLKHVLPSLGIRRFGFMSGVSPEQVEVQLRRAGLFDAVGMIYLE
CCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCHHHHCCEEEEE
TPANPTNALVDIAGFAALAKRMPRRVLTAVDNTFLGPLWQHPLAHGADLVLYSATKYIGG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHHCCCCEEEEEHHHHCCC
HSDVIAGVCLGSRELLAQVKGMRTIMGTMAGAHTGWLLLRSLETLKIRMETQQAGARRVA
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHHHHHHHHH
DFLATHPKVARVYYLGHLESNDPQYHIYRRQCLGPGAMISFDLHGGEAEAFRFLNHLQLI
HHHHCCCCEEEEEEEEEECCCCCCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
HLAVSLGGTESLAEHPASMTHSDVDPEERIEFGIGPAMIRLSVGVEHPDDLIADLNQALR
HHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
AV
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]