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The map label for this gene is yfkN [H]

Identifier: 222526745

GI number: 222526745

Start: 4382466

End: 4384265

Strand: Direct

Name: yfkN [H]

Synonym: Chy400_3519

Alternate gene names: 222526745

Gene position: 4382466-4384265 (Clockwise)

Preceding gene: 222526733

Following gene: 222526749

Centisome position: 83.18

GC content: 56.94

Gene sequence:

>1800_bases
ATGGAAAAGATCTCGCGTCGGCGGTTTCTCAAGGGGACGGTCGCTCTGGGAGCTGGTGCATTGCTGTCGATCTACAGCGA
CGGCAGCTTTCGGTTGGCGTTAGCGCAGGAGAATCCGGTCTTTCGTATGCGGGTGTTGCATACCAATGACCATCACGCCC
GCATTGAACCGGTATTCAGTGGGAATAACCCGGTTCACGGTGGTGTTTCGCGACGCAAAGCACTGATTGACAAGATTCGA
CGGGAAACGGCATTACCAACCTTGCTGGTTGATGCCGGTGACGTTTTTCAGGGAACACTGTACTTCAATCAGTATAACGG
GATGGCCGATCTCGAATTCTACAATGCGATGGGATACGAGGCGATGGCCATCGGCAACCACGAGTTTGATAAAGGGCCGC
AGGCACTGGTAGATTTCATCACCCGGGCCAAATTTCCGGTATTGAGCGCCAATATCAGCGTTGCCGCCGGTAATCCGCTG
GCCGGTCTGATCAAGCCACGTACCATTATCGAAAAAGATGGCAGGAAGATTGGTATCTTCAGTCTGACTCCGGAAGATAC
TGGGGTGCTCTCGAATGCCGGGCCGGGTATTAGTTTTACTTCGGCGATTGAAGCCGCCCGGCAACAGGTGGCTGCCTTGA
AAGCCGAAGGGGTGTTTACCATTATTGCCCTGACCCACGTTGGAATTAACGTTGATCGCCAGATTGCTCGTGAAGTCGGG
GGGATGAGCCTGATTATCGGCGGTCACTCCCATACCCCAATGGCGCCAATGAACAACGTCAAGACGCCGCCGTACCCCGA
ACTGATTGCCGGTCCCGATGGGAAGCCGGTGGTTGTGGTGACCGATTGGGAGTGGGGGCGCTGGTTGGGTGATATTACGG
TTGCCTTCAACGCTTCCGGTACCGTGATCGATCTACAGGGTAACCCGACCGAAATCGTACCATCACTCACCGCCGATCAA
GGCTTCGAGAACCGGATCGCCGTCTTCCGTGGCCCAATCGATCAGTTGCGGGCACGTGTGGTTGGTTCTACCGCGGTTGA
GCTTGATGGTAGTCGGACAAATGTTCGCTCTCGTGAAACCAACCTGGGGAATTTGATCGCCGATGCGATGCTGGCTAAAG
TGCGCAATTCGGGGGCAACGATTGCGATTATGAATGGTGGTGGTATTCGCACCTCGATCCCGGCTGGACCGATTACGGTT
GGTCAAATCCTGGAAGTGTTGCCATTCGGTAACACCCTGGCGCTGGTCACCCTGACCGGTGCGCAGGTGATAGAGGCGTT
GAATAATGGTGTGAGCCAGGTGGAGAGTGGTGCCGGTCGCTTCCCGCAGGTTGCCGGAATTCGCTTCACCTACGATCCTT
CTCTCCCGGCAAGTGGCCGGGTTACCAGCGTGACGGTCGGTGGTTCACCGATTGATCCAGGTGCCGACTATATCGTGGTA
ACTAATAACTTCCTGCTGGCGGGTGGTGATGGCTACAGCGTCTTTACCCGTGGGCGCAACCAGGTAGACACCGGCTTTAT
CCTGGCCGATGTGGTAGAGGAGTATGTTGCGGCGAACTCACCGCTTAATCCGACAGTCGATGGCCGGATTGCAGTTGGCG
CTGCGCCGGCAACAACTCCGGCTCAGCCGACCGCACCGACTCCGGCGACGTTACCCAATACTGGTGGCGCGCTGACTCCG
CTGGCGTGGCTGGCCGGATTGGGTGCAGCCGCACTGGCCGGTGGTGCTGCCTTGCAACGCTCCGCCGCTGAAAGCCCCGA
AAAGGAAGAGGTTGACACAGACGTGGAGGCGGTTCGCTAA

Upstream 100 bases:

>100_bases
CGGTTTGCGTGTCTTGGGAAGATCGGCCTGGCTACCGGCTATCACCCGGTGCCCAGGGTCGGTTCTGGTTGTTCGTGACT
TGAAACGGAGAGCATGTCCT

Downstream 100 bases:

>100_bases
ACCGGTTCGGTTCGTAACTACGGCAAAGGGGCGCGACTCGCGCCCCTTTGCTCTTCCACTCGTGGTGCCGGCAGGAAGCC
GCGACCGGCAATGTGGTACT

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MEKISRRRFLKGTVALGAGALLSIYSDGSFRLALAQENPVFRMRVLHTNDHHARIEPVFSGNNPVHGGVSRRKALIDKIR
RETALPTLLVDAGDVFQGTLYFNQYNGMADLEFYNAMGYEAMAIGNHEFDKGPQALVDFITRAKFPVLSANISVAAGNPL
AGLIKPRTIIEKDGRKIGIFSLTPEDTGVLSNAGPGISFTSAIEAARQQVAALKAEGVFTIIALTHVGINVDRQIAREVG
GMSLIIGGHSHTPMAPMNNVKTPPYPELIAGPDGKPVVVVTDWEWGRWLGDITVAFNASGTVIDLQGNPTEIVPSLTADQ
GFENRIAVFRGPIDQLRARVVGSTAVELDGSRTNVRSRETNLGNLIADAMLAKVRNSGATIAIMNGGGIRTSIPAGPITV
GQILEVLPFGNTLALVTLTGAQVIEALNNGVSQVESGAGRFPQVAGIRFTYDPSLPASGRVTSVTVGGSPIDPGADYIVV
TNNFLLAGGDGYSVFTRGRNQVDTGFILADVVEEYVAANSPLNPTVDGRIAVGAAPATTPAQPTAPTPATLPNTGGALTP
LAWLAGLGAAALAGGAALQRSAAESPEKEEVDTDVEAVR

Sequences:

>Translated_599_residues
MEKISRRRFLKGTVALGAGALLSIYSDGSFRLALAQENPVFRMRVLHTNDHHARIEPVFSGNNPVHGGVSRRKALIDKIR
RETALPTLLVDAGDVFQGTLYFNQYNGMADLEFYNAMGYEAMAIGNHEFDKGPQALVDFITRAKFPVLSANISVAAGNPL
AGLIKPRTIIEKDGRKIGIFSLTPEDTGVLSNAGPGISFTSAIEAARQQVAALKAEGVFTIIALTHVGINVDRQIAREVG
GMSLIIGGHSHTPMAPMNNVKTPPYPELIAGPDGKPVVVVTDWEWGRWLGDITVAFNASGTVIDLQGNPTEIVPSLTADQ
GFENRIAVFRGPIDQLRARVVGSTAVELDGSRTNVRSRETNLGNLIADAMLAKVRNSGATIAIMNGGGIRTSIPAGPITV
GQILEVLPFGNTLALVTLTGAQVIEALNNGVSQVESGAGRFPQVAGIRFTYDPSLPASGRVTSVTVGGSPIDPGADYIVV
TNNFLLAGGDGYSVFTRGRNQVDTGFILADVVEEYVAANSPLNPTVDGRIAVGAAPATTPAQPTAPTPATLPNTGGALTP
LAWLAGLGAAALAGGAALQRSAAESPEKEEVDTDVEAVR
>Mature_599_residues
MEKISRRRFLKGTVALGAGALLSIYSDGSFRLALAQENPVFRMRVLHTNDHHARIEPVFSGNNPVHGGVSRRKALIDKIR
RETALPTLLVDAGDVFQGTLYFNQYNGMADLEFYNAMGYEAMAIGNHEFDKGPQALVDFITRAKFPVLSANISVAAGNPL
AGLIKPRTIIEKDGRKIGIFSLTPEDTGVLSNAGPGISFTSAIEAARQQVAALKAEGVFTIIALTHVGINVDRQIAREVG
GMSLIIGGHSHTPMAPMNNVKTPPYPELIAGPDGKPVVVVTDWEWGRWLGDITVAFNASGTVIDLQGNPTEIVPSLTADQ
GFENRIAVFRGPIDQLRARVVGSTAVELDGSRTNVRSRETNLGNLIADAMLAKVRNSGATIAIMNGGGIRTSIPAGPITV
GQILEVLPFGNTLALVTLTGAQVIEALNNGVSQVESGAGRFPQVAGIRFTYDPSLPASGRVTSVTVGGSPIDPGADYIVV
TNNFLLAGGDGYSVFTRGRNQVDTGFILADVVEEYVAANSPLNPTVDGRIAVGAAPATTPAQPTAPTPATLPNTGGALTP
LAWLAGLGAAALAGGAALQRSAAESPEKEEVDTDVEAVR

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=524, Percent_Identity=36.0687022900763, Blast_Score=278, Evalue=1e-74,
Organism=Escherichia coli, GI1786687, Length=558, Percent_Identity=31.8996415770609, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1790658, Length=541, Percent_Identity=23.2902033271719, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922446, Length=533, Percent_Identity=32.6454033771107, Blast_Score=252, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24654424, Length=533, Percent_Identity=32.6454033771107, Blast_Score=252, Evalue=6e-67,
Organism=Drosophila melanogaster, GI19922444, Length=543, Percent_Identity=31.8600368324125, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI28573524, Length=531, Percent_Identity=30.1318267419962, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24641187, Length=535, Percent_Identity=30.6542056074766, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI221329836, Length=525, Percent_Identity=30.8571428571429, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI19921980, Length=466, Percent_Identity=28.755364806867, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI161076508, Length=466, Percent_Identity=28.755364806867, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24652512, Length=466, Percent_Identity=28.755364806867, Blast_Score=169, Evalue=6e-42,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 62802; Mature: 62802

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKISRRRFLKGTVALGAGALLSIYSDGSFRLALAQENPVFRMRVLHTNDHHARIEPVFS
CCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEEEEC
GNNPVHGGVSRRKALIDKIRRETALPTLLVDAGDVFQGTLYFNQYNGMADLEFYNAMGYE
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCEEEEEHHHCCCCE
AMAIGNHEFDKGPQALVDFITRAKFPVLSANISVAAGNPLAGLIKPRTIIEKDGRKIGIF
EEEECCCCCCCCHHHHHHHHHHCCCCEEECCEEEECCCCCCHHCCCCHHEECCCCEEEEE
SLTPEDTGVLSNAGPGISFTSAIEAARQQVAALKAEGVFTIIALTHVGINVDRQIAREVG
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHC
GMSLIIGGHSHTPMAPMNNVKTPPYPELIAGPDGKPVVVVTDWEWGRWLGDITVAFNASG
CEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEEEEEECCC
TVIDLQGNPTEIVPSLTADQGFENRIAVFRGPIDQLRARVVGSTAVELDGSRTNVRSRET
CEEEECCCCCEECCCCCCCCCCCCCEEEEECCHHHHHHHHHCCEEEEECCCCCCCCCCCC
NLGNLIADAMLAKVRNSGATIAIMNGGGIRTSIPAGPITVGQILEVLPFGNTLALVTLTG
CHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHCCCCEEEEEEECH
AQVIEALNNGVSQVESGAGRFPQVAGIRFTYDPSLPASGRVTSVTVGGSPIDPGADYIVV
HHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEE
TNNFLLAGGDGYSVFTRGRNQVDTGFILADVVEEYVAANSPLNPTVDGRIAVGAAPATTP
ECCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
AQPTAPTPATLPNTGGALTPLAWLAGLGAAALAGGAALQRSAAESPEKEEVDTDVEAVR
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCHHHCCCHHHHCC
>Mature Secondary Structure
MEKISRRRFLKGTVALGAGALLSIYSDGSFRLALAQENPVFRMRVLHTNDHHARIEPVFS
CCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCEEEEEEEECCCCCEEEEEEEC
GNNPVHGGVSRRKALIDKIRRETALPTLLVDAGDVFQGTLYFNQYNGMADLEFYNAMGYE
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEEECCCEEEEEHHHCCCCE
AMAIGNHEFDKGPQALVDFITRAKFPVLSANISVAAGNPLAGLIKPRTIIEKDGRKIGIF
EEEECCCCCCCCHHHHHHHHHHCCCCEEECCEEEECCCCCCHHCCCCHHEECCCCEEEEE
SLTPEDTGVLSNAGPGISFTSAIEAARQQVAALKAEGVFTIIALTHVGINVDRQIAREVG
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHC
GMSLIIGGHSHTPMAPMNNVKTPPYPELIAGPDGKPVVVVTDWEWGRWLGDITVAFNASG
CEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEEEEEECCC
TVIDLQGNPTEIVPSLTADQGFENRIAVFRGPIDQLRARVVGSTAVELDGSRTNVRSRET
CEEEECCCCCEECCCCCCCCCCCCCEEEEECCHHHHHHHHHCCEEEEECCCCCCCCCCCC
NLGNLIADAMLAKVRNSGATIAIMNGGGIRTSIPAGPITVGQILEVLPFGNTLALVTLTG
CHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHHCCCCEEEEEEECH
AQVIEALNNGVSQVESGAGRFPQVAGIRFTYDPSLPASGRVTSVTVGGSPIDPGADYIVV
HHHHHHHHCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEE
TNNFLLAGGDGYSVFTRGRNQVDTGFILADVVEEYVAANSPLNPTVDGRIAVGAAPATTP
ECCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
AQPTAPTPATLPNTGGALTPLAWLAGLGAAALAGGAALQRSAAESPEKEEVDTDVEAVR
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHHHCCCCCHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]