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The map label for this gene is yfeW [H]

Identifier: 222526676

GI number: 222526676

Start: 4297792

End: 4298841

Strand: Direct

Name: yfeW [H]

Synonym: Chy400_3446

Alternate gene names: 222526676

Gene position: 4297792-4298841 (Clockwise)

Preceding gene: 222526675

Following gene: 222526677

Centisome position: 81.57

GC content: 62.1

Gene sequence:

>1050_bases
ATGATACCTGAGATTGATCACATCATCACCACCGCGATTCACGAGCGTATCTTTCCAGGCGCCATTGTCCTGGTGGCGCA
GGACACCCACATCCTGCATGCGGCTGCTTATGGCACCACGATGTACGATGACCCCGGCACCATGCCGGTCACGCTCGATA
CCATCTACGATCTGGCGTCGTTAACCAAAGTCTTCACCGCCACCGCCGCACTCCGCCTGCACGACGCCGGCATGCTACCA
CTCGACCAACCCATCCAGCACTGGCTACCGGCACTGCATGCACCCGACATCACCGTTCGCCATCTCCTGAGTCACTCCAG
TGGTCTGACCGTCCAACTGGCGCCCTTAGCCCGCGCCGGCGCCGCAACGATCAGAACAATGGTCTACACAGCCACTCCCC
GCCATCCACCCGGTCGCATCGTTGAGTATGCCAACCTCAACACCCTGTTGTTGGGTGATGTCGTGACCGCGATCTACGGT
GGTTCACTGGCCGACGCCCTGACCGAACTGGTCTGTGTACCGCTCGGCCTGCACAAGACGCGCTTCAACCCACCGACGGA
GTGGCGGGCAACCATTGCCCCCACCGAATGGGACTGGGAATGGCGTGGTGGCCTTGTACACGGCGTGGTGCATGACGAAA
GCACGTATGCGCTGGGCGGCGTTGCCGGTCATGCCGGCCTCTTCGGCCCGGCGGCTGAAGCATTGCGGCTGGTACAGCTC
TTCATCCAGGAAGGAGCCTGGCAAGACCGCCAGCTCCTGCGGGCCGGCACAGCACTGGCTGCACTAACCCCACAACCGGC
CTCCGACACCAGCCGATTGGTGAGTGGCCTGGGCTGGATGCTACACCGCCCATACATGGGGAGCGCCATCCACCACGGCT
TCGGCCATAGTGGCTTCACCGGCCCCCTCCTGATCGGCATTCCCCAGCGGAGACTGGCAATCGTCCTGCTGTGCAACCGC
ACCTACCCCAAACGAACACCGCCGCCGTACCGTCACCATGCGATTGCTGCTATGGTTGTAGACGCAATCATCCAGGCGAT
AGATCGTTAG

Upstream 100 bases:

>100_bases
GTATCGGCTTGAGCCGCACCGATTCCTTCACCTCACAACAGGCGTATACTTTCAGGAAAGCGTCAGCAGATAAGGGAGCA
ACCTTCGTACCCACAACGAT

Downstream 100 bases:

>100_bases
CGCTCTTGCCAGCCAGATTGCCATAAGTACGGGTATAATGATAGAGAGCGATAAAACCGTTTGATTCAACCTCTTCCAGG
AGAGTGATATGAGTCGCAAA

Product: beta-lactamase

Products: substituted beta-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIPEIDHIITTAIHERIFPGAIVLVAQDTHILHAAAYGTTMYDDPGTMPVTLDTIYDLASLTKVFTATAALRLHDAGMLP
LDQPIQHWLPALHAPDITVRHLLSHSSGLTVQLAPLARAGAATIRTMVYTATPRHPPGRIVEYANLNTLLLGDVVTAIYG
GSLADALTELVCVPLGLHKTRFNPPTEWRATIAPTEWDWEWRGGLVHGVVHDESTYALGGVAGHAGLFGPAAEALRLVQL
FIQEGAWQDRQLLRAGTALAALTPQPASDTSRLVSGLGWMLHRPYMGSAIHHGFGHSGFTGPLLIGIPQRRLAIVLLCNR
TYPKRTPPPYRHHAIAAMVVDAIIQAIDR

Sequences:

>Translated_349_residues
MIPEIDHIITTAIHERIFPGAIVLVAQDTHILHAAAYGTTMYDDPGTMPVTLDTIYDLASLTKVFTATAALRLHDAGMLP
LDQPIQHWLPALHAPDITVRHLLSHSSGLTVQLAPLARAGAATIRTMVYTATPRHPPGRIVEYANLNTLLLGDVVTAIYG
GSLADALTELVCVPLGLHKTRFNPPTEWRATIAPTEWDWEWRGGLVHGVVHDESTYALGGVAGHAGLFGPAAEALRLVQL
FIQEGAWQDRQLLRAGTALAALTPQPASDTSRLVSGLGWMLHRPYMGSAIHHGFGHSGFTGPLLIGIPQRRLAIVLLCNR
TYPKRTPPPYRHHAIAAMVVDAIIQAIDR
>Mature_349_residues
MIPEIDHIITTAIHERIFPGAIVLVAQDTHILHAAAYGTTMYDDPGTMPVTLDTIYDLASLTKVFTATAALRLHDAGMLP
LDQPIQHWLPALHAPDITVRHLLSHSSGLTVQLAPLARAGAATIRTMVYTATPRHPPGRIVEYANLNTLLLGDVVTAIYG
GSLADALTELVCVPLGLHKTRFNPPTEWRATIAPTEWDWEWRGGLVHGVVHDESTYALGGVAGHAGLFGPAAEALRLVQL
FIQEGAWQDRQLLRAGTALAALTPQPASDTSRLVSGLGWMLHRPYMGSAIHHGFGHSGFTGPLLIGIPQRRLAIVLLCNR
TYPKRTPPPYRHHAIAAMVVDAIIQAIDR

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0214 family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=383, Percent_Identity=30.0261096605744, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786574, Length=351, Percent_Identity=24.2165242165242, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR022849 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: 3.5.2.6

Molecular weight: Translated: 37823; Mature: 37823

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPEIDHIITTAIHERIFPGAIVLVAQDTHILHAAAYGTTMYDDPGTMPVTLDTIYDLAS
CCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCEEECCCCCCEEEHHHHHHHHH
LTKVFTATAALRLHDAGMLPLDQPIQHWLPALHAPDITVRHLLSHSSGLTVQLAPLARAG
HHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCHHCC
AATIRTMVYTATPRHPPGRIVEYANLNTLLLGDVVTAIYGGSLADALTELVCVPLGLHKT
HHHHHEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHC
RFNPPTEWRATIAPTEWDWEWRGGLVHGVVHDESTYALGGVAGHAGLFGPAAEALRLVQL
CCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHH
FIQEGAWQDRQLLRAGTALAALTPQPASDTSRLVSGLGWMLHRPYMGSAIHHGFGHSGFT
HHHHCCCHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCC
GPLLIGIPQRRLAIVLLCNRTYPKRTPPPYRHHAIAAMVVDAIIQAIDR
CCEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPEIDHIITTAIHERIFPGAIVLVAQDTHILHAAAYGTTMYDDPGTMPVTLDTIYDLAS
CCCCHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCEEECCCCCCEEEHHHHHHHHH
LTKVFTATAALRLHDAGMLPLDQPIQHWLPALHAPDITVRHLLSHSSGLTVQLAPLARAG
HHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCHHCC
AATIRTMVYTATPRHPPGRIVEYANLNTLLLGDVVTAIYGGSLADALTELVCVPLGLHKT
HHHHHEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHC
RFNPPTEWRATIAPTEWDWEWRGGLVHGVVHDESTYALGGVAGHAGLFGPAAEALRLVQL
CCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHH
FIQEGAWQDRQLLRAGTALAALTPQPASDTSRLVSGLGWMLHRPYMGSAIHHGFGHSGFT
HHHHCCCHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCC
GPLLIGIPQRRLAIVLLCNRTYPKRTPPPYRHHAIAAMVVDAIIQAIDR
CCEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-lactam; H2O

Specific reaction: A beta-lactam + H2O = a substituted beta-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]