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The map label for this gene is sigA [H]

Identifier: 222526666

GI number: 222526666

Start: 4284978

End: 4286060

Strand: Reverse

Name: sigA [H]

Synonym: Chy400_3435

Alternate gene names: 222526666

Gene position: 4286060-4284978 (Counterclockwise)

Preceding gene: 222526667

Following gene: 222526663

Centisome position: 81.35

GC content: 58.36

Gene sequence:

>1083_bases
GTGAAAGCCTTCCCTCAAAAGCACGCAGCCGATAGTGAAATGGAGCCGGAATTGTCCGCGGAGGCCACCGAACTCGATGC
TGAGTGGGATGGCGATGTTGATCCCGAACCAGAGGATCTCGAGGTAGCAGAACTGGAGCCACTCGATGACCTCGAGAGCG
AGGGTGATTTTGAGCCAGTTGAGGATTCGGTACAGCTCTACTTGCAAGAGATCGGTCAGGTTCCACTGCTTACCGCCGAA
GAGGAAGTAGAGCTGGCCCGGCAGTACAGTCGCGGTAAAGAGGCACGGCAACGTCTACGCAACGAGGATTTCGCCAACGA
CCGCGAACGGATGCGGCTGGAGATGGAAATGGCTCGCGGTGAAGAGGCGCGGCGGCGCCTGATTCAGGCCAATCTGCGCC
TGGTGGTGAGTGTGGCGAAGAAGTATATCGGCAGTCCGATGGCCTTCATGGATCTCGTGCAAGAGGGGAACATCGGCCTG
ATGCGTGCAGTCGAGAAGTTTGACTACACGAAGGGTAATCGCTTCAGCACCTATGCCACCTGGTGGATCCGCCAGGCCGT
AACCAGGGCTATCGCCGAGCAGAGCCGTCTTATCCGTCTCCCCGTTCATCTCAGCGAGTCGATTGTGCATCTGCGCCGCG
CCATCTATCGCCTTGAACAGGAGCTGGAGCGCGAACCAACTGCCGAGGAGCTGGCTCACGCCCTCAATATGAGTGTGCGC
AAAGTCAAGCGCCTGATCCGCGCTTCAACTCAGCCTATCTCGCTCGAACAACCGCTCAACAACGAGCAGGAAGGTCGGGT
GAGCGAAACACTGGCTGATGAGACGGTGGCTTCACCGATGGAGATCGCCGAGCAGAAGATGCTTCACGCCGAGTTAACGG
CTGCTCTGAACGAGCTGCCGGAACGTGAACGCAAGATTCTCCAGTTGCGCTACGGCTTGATTGATGGTCAACGCCGCACC
CTCGAAGAGGTTGGGGCAACGTTTGGCATCACGCGCGAACGCACCCGCCAGATCGAGGCCGAAGCGTTACGTCGCCTGCG
CCATCCCAGCGTTGGTCAGCGGTTACATAGCTATCTTGACTAG

Upstream 100 bases:

>100_bases
AATCTTTGAGGAGGCGTATATCCAGATAGCGAATTATTAACGCCTCGCAAAGAACGGTCGCGGAATCGTTGCCGTCAACC
GGAGATGAAGGAGTACTGCC

Downstream 100 bases:

>100_bases
AAGTGGTCTCACAAACAGATACCGGGAGAACAGCGAAGGGCGACATGTGTGTCGCCCTTCTCGCATTATGTCACATCTGA
CAGCACGCGCCGCTAACGCT

Product: RpoD subfamily RNA polymerase sigma-70 subunit

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKAFPQKHAADSEMEPELSAEATELDAEWDGDVDPEPEDLEVAELEPLDDLESEGDFEPVEDSVQLYLQEIGQVPLLTAE
EEVELARQYSRGKEARQRLRNEDFANDRERMRLEMEMARGEEARRRLIQANLRLVVSVAKKYIGSPMAFMDLVQEGNIGL
MRAVEKFDYTKGNRFSTYATWWIRQAVTRAIAEQSRLIRLPVHLSESIVHLRRAIYRLEQELEREPTAEELAHALNMSVR
KVKRLIRASTQPISLEQPLNNEQEGRVSETLADETVASPMEIAEQKMLHAELTAALNELPERERKILQLRYGLIDGQRRT
LEEVGATFGITRERTRQIEAEALRRLRHPSVGQRLHSYLD

Sequences:

>Translated_360_residues
MKAFPQKHAADSEMEPELSAEATELDAEWDGDVDPEPEDLEVAELEPLDDLESEGDFEPVEDSVQLYLQEIGQVPLLTAE
EEVELARQYSRGKEARQRLRNEDFANDRERMRLEMEMARGEEARRRLIQANLRLVVSVAKKYIGSPMAFMDLVQEGNIGL
MRAVEKFDYTKGNRFSTYATWWIRQAVTRAIAEQSRLIRLPVHLSESIVHLRRAIYRLEQELEREPTAEELAHALNMSVR
KVKRLIRASTQPISLEQPLNNEQEGRVSETLADETVASPMEIAEQKMLHAELTAALNELPERERKILQLRYGLIDGQRRT
LEEVGATFGITRERTRQIEAEALRRLRHPSVGQRLHSYLD
>Mature_360_residues
MKAFPQKHAADSEMEPELSAEATELDAEWDGDVDPEPEDLEVAELEPLDDLESEGDFEPVEDSVQLYLQEIGQVPLLTAE
EEVELARQYSRGKEARQRLRNEDFANDRERMRLEMEMARGEEARRRLIQANLRLVVSVAKKYIGSPMAFMDLVQEGNIGL
MRAVEKFDYTKGNRFSTYATWWIRQAVTRAIAEQSRLIRLPVHLSESIVHLRRAIYRLEQELEREPTAEELAHALNMSVR
KVKRLIRASTQPISLEQPLNNEQEGRVSETLADETVASPMEIAEQKMLHAELTAALNELPERERKILQLRYGLIDGQRRT
LEEVGATFGITRERTRQIEAEALRRLRHPSVGQRLHSYLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=249, Percent_Identity=46.9879518072289, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789098, Length=316, Percent_Identity=43.0379746835443, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=26.7489711934156, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788231, Length=198, Percent_Identity=28.7878787878788, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001909
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41508; Mature: 41508

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFPQKHAADSEMEPELSAEATELDAEWDGDVDPEPEDLEVAELEPLDDLESEGDFEPV
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHCCCCCCCCH
EDSVQLYLQEIGQVPLLTAEEEVELARQYSRGKEARQRLRNEDFANDRERMRLEMEMARG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCC
EEARRRLIQANLRLVVSVAKKYIGSPMAFMDLVQEGNIGLMRAVEKFDYTKGNRFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHH
WWIRQAVTRAIAEQSRLIRLPVHLSESIVHLRRAIYRLEQELEREPTAEELAHALNMSVR
HHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KVKRLIRASTQPISLEQPLNNEQEGRVSETLADETVASPMEIAEQKMLHAELTAALNELP
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
ERERKILQLRYGLIDGQRRTLEEVGATFGITRERTRQIEAEALRRLRHPSVGQRLHSYLD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MKAFPQKHAADSEMEPELSAEATELDAEWDGDVDPEPEDLEVAELEPLDDLESEGDFEPV
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHCCCCCCCCH
EDSVQLYLQEIGQVPLLTAEEEVELARQYSRGKEARQRLRNEDFANDRERMRLEMEMARG
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHCC
EEARRRLIQANLRLVVSVAKKYIGSPMAFMDLVQEGNIGLMRAVEKFDYTKGNRFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHH
WWIRQAVTRAIAEQSRLIRLPVHLSESIVHLRRAIYRLEQELEREPTAEELAHALNMSVR
HHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KVKRLIRASTQPISLEQPLNNEQEGRVSETLADETVASPMEIAEQKMLHAELTAALNELP
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
ERERKILQLRYGLIDGQRRTLEEVGATFGITRERTRQIEAEALRRLRHPSVGQRLHSYLD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA