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The map label for this gene is phoE [H]

Identifier: 222526645

GI number: 222526645

Start: 4254717

End: 4255346

Strand: Reverse

Name: phoE [H]

Synonym: Chy400_3414

Alternate gene names: 222526645

Gene position: 4255346-4254717 (Counterclockwise)

Preceding gene: 222526646

Following gene: 222526644

Centisome position: 80.76

GC content: 58.73

Gene sequence:

>630_bases
ATGCGTCTCATTCTTGTGCGACACGGCGAGACGCCGTGGAATCAAACGTTACAGTACCAGGGTCACGCGCCGATACCGCT
CAACGAGCGTGGGCGCGAACAGGCCCGACGGGCAGGGATACGATTGGTTCGGAGCGGGGCAGTTGCTCTGTACAGTAGCG
ATCTGCCCCGCGCATGGGAGACAGCCGAGATTATTGGGTCACACGTCAATCTGCAACCGGTTGCTATGCCGGATTTGCGC
GAGATTGATGTCGGGTTATGGGAAGGGCTAACCCCCGACGAACTCTATCAGCGCTTCCCCGACCATATGCGCGAATACGA
CCGCGACCCGGCACGAACTGTGCGTCTCGGCGGCGAAAGTTATGCCCAATTGCAAGCAAGGGTGCTGCGGGCGTTTGCCC
GGATCGAGGCCGCTCACCGCAATGGTGAGACGATTATCGTGGTTTCGCATGGCGGCTCCATCCGCGCCTTGTTCTGTCAC
ATCATCGGTCTCGATCTGGCAAACTTTAGCAAATTGTGGCTCGACAATGGTTCGTTGAGCGAAATCGTCCGCGGGCGAAG
TGGCTGGCGGCTATTACGGATGAATGATGTCGCCCACCTTGAGGAACTGGTGGCGGAAGGCGGCGAGTAA

Upstream 100 bases:

>100_bases
GATTTACGAAGGGACAAGTGAGATTCAGCGCCTCGTGATCAGCCGCGAACTGGTACGCTGATCAGAGTAGACGGCGGCGC
TGCGTCGCCCAACAGGAGTT

Downstream 100 bases:

>100_bases
ACGTATGAGCGGACGAACCACTGCTAACGAACATGATCTGTTATCGGCAAACATTCGCGCACTAGGCGATGCACTTGGCC
GGGTCATTATCGGTCAACAC

Product: phosphoglycerate mutase

Products: 3-phospho-D-glycerate

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MRLILVRHGETPWNQTLQYQGHAPIPLNERGREQARRAGIRLVRSGAVALYSSDLPRAWETAEIIGSHVNLQPVAMPDLR
EIDVGLWEGLTPDELYQRFPDHMREYDRDPARTVRLGGESYAQLQARVLRAFARIEAAHRNGETIIVVSHGGSIRALFCH
IIGLDLANFSKLWLDNGSLSEIVRGRSGWRLLRMNDVAHLEELVAEGGE

Sequences:

>Translated_209_residues
MRLILVRHGETPWNQTLQYQGHAPIPLNERGREQARRAGIRLVRSGAVALYSSDLPRAWETAEIIGSHVNLQPVAMPDLR
EIDVGLWEGLTPDELYQRFPDHMREYDRDPARTVRLGGESYAQLQARVLRAFARIEAAHRNGETIIVVSHGGSIRALFCH
IIGLDLANFSKLWLDNGSLSEIVRGRSGWRLLRMNDVAHLEELVAEGGE
>Mature_209_residues
MRLILVRHGETPWNQTLQYQGHAPIPLNERGREQARRAGIRLVRSGAVALYSSDLPRAWETAEIIGSHVNLQPVAMPDLR
EIDVGLWEGLTPDELYQRFPDHMREYDRDPARTVRLGGESYAQLQARVLRAFARIEAAHRNGETIIVVSHGGSIRALFCH
IIGLDLANFSKLWLDNGSLSEIVRGRSGWRLLRMNDVAHLEELVAEGGE

Specific function: Phosphatase with broad substrate specificity. Does not have phosphoglycerate mutase activtiy [H]

COG id: COG0406

COG function: function code G; Fructose-2,6-bisphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate mutase family. GpmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786857, Length=182, Percent_Identity=32.967032967033, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1790856, Length=206, Percent_Identity=30.5825242718447, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322896, Length=188, Percent_Identity=28.7234042553192, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013078
- InterPro:   IPR001345 [H]

Pfam domain/function: PF00300 PGAM [H]

EC number: 5.4.2.1

Molecular weight: Translated: 23587; Mature: 23587

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00175 PG_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLILVRHGETPWNQTLQYQGHAPIPLNERGREQARRAGIRLVRSGAVALYSSDLPRAWE
CEEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHH
TAEIIGSHVNLQPVAMPDLREIDVGLWEGLTPDELYQRFPDHMREYDRDPARTVRLGGES
HHHHHHCCCCCCCCCCCCHHHHCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHEEEECCHH
YAQLQARVLRAFARIEAAHRNGETIIVVSHGGSIRALFCHIIGLDLANFSKLWLDNGSLS
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCCCCHH
EIVRGRSGWRLLRMNDVAHLEELVAEGGE
HHHCCCCCCEEEEECCHHHHHHHHHCCCC
>Mature Secondary Structure
MRLILVRHGETPWNQTLQYQGHAPIPLNERGREQARRAGIRLVRSGAVALYSSDLPRAWE
CEEEEEECCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHH
TAEIIGSHVNLQPVAMPDLREIDVGLWEGLTPDELYQRFPDHMREYDRDPARTVRLGGES
HHHHHHCCCCCCCCCCCCHHHHCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHEEEECCHH
YAQLQARVLRAFARIEAAHRNGETIIVVSHGGSIRALFCHIIGLDLANFSKLWLDNGSLS
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHCCCCCHH
EIVRGRSGWRLLRMNDVAHLEELVAEGGE
HHHCCCCCCEEEEECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-phospho-D-glycerate

Specific reaction: 2-phospho-D-glycerate = 3-phospho-D-glycerate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]